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Detection of dietetically absorbed maize-derived microRNAs in pigs

MicroRNAs are a class of small RNAs that are important in post-transcriptional gene regulation in animals and plants. These single-stranded molecules are widely distributed in organisms and influence fundamental biological processes. Interestingly, recent studies have reported that diet-derived plan...

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Autores principales: Luo, Yi, Wang, Pengjun, Wang, Xun, Wang, Yuhao, Mu, Zhiping, Li, Qingzhi, Fu, Yuhua, Xiao, Juan, Li, Guojun, Ma, Yao, Gu, Yiren, Jin, Long, Ma, Jideng, Tang, Qianzi, Jiang, Anan, Li, Xuewei, Li, Mingzhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428504/
https://www.ncbi.nlm.nih.gov/pubmed/28381865
http://dx.doi.org/10.1038/s41598-017-00488-y
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author Luo, Yi
Wang, Pengjun
Wang, Xun
Wang, Yuhao
Mu, Zhiping
Li, Qingzhi
Fu, Yuhua
Xiao, Juan
Li, Guojun
Ma, Yao
Gu, Yiren
Jin, Long
Ma, Jideng
Tang, Qianzi
Jiang, Anan
Li, Xuewei
Li, Mingzhou
author_facet Luo, Yi
Wang, Pengjun
Wang, Xun
Wang, Yuhao
Mu, Zhiping
Li, Qingzhi
Fu, Yuhua
Xiao, Juan
Li, Guojun
Ma, Yao
Gu, Yiren
Jin, Long
Ma, Jideng
Tang, Qianzi
Jiang, Anan
Li, Xuewei
Li, Mingzhou
author_sort Luo, Yi
collection PubMed
description MicroRNAs are a class of small RNAs that are important in post-transcriptional gene regulation in animals and plants. These single-stranded molecules are widely distributed in organisms and influence fundamental biological processes. Interestingly, recent studies have reported that diet-derived plant miRNAs could regulate mammalian gene expression, and these studies have broadened our view of cross-kingdom communication. In the present study, we evaluated miRNA levels in cooked maize-containing chow diets, and found that plant miRNAs were resistant to the harsh cooking conditions to a certain extent. After feeding fresh maize to pigs (7 days), maize-derived miRNAs could be detected in porcine tissues and serum, and the authenticity of these plant miRNAs was confirmed by using oxidization reactions. Furthermore, in vivo and in vitro experiments demonstrated that dietary maize miRNAs could cross the gastrointestinal tract and enter the porcine bloodstream. In the porcine cells, we found that plant miRNAs are very likely to specifically target their endogenous porcine mRNAs and influence gene expression in a fashion similar to that of mammalian miRNAs. Our results indicate that maize-derived miRNAs can cross the gastrointestinal tract and present in pigs, and these exogenous miRNAs have the potential to regulate mammalian gene expression.
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spelling pubmed-54285042017-05-15 Detection of dietetically absorbed maize-derived microRNAs in pigs Luo, Yi Wang, Pengjun Wang, Xun Wang, Yuhao Mu, Zhiping Li, Qingzhi Fu, Yuhua Xiao, Juan Li, Guojun Ma, Yao Gu, Yiren Jin, Long Ma, Jideng Tang, Qianzi Jiang, Anan Li, Xuewei Li, Mingzhou Sci Rep Article MicroRNAs are a class of small RNAs that are important in post-transcriptional gene regulation in animals and plants. These single-stranded molecules are widely distributed in organisms and influence fundamental biological processes. Interestingly, recent studies have reported that diet-derived plant miRNAs could regulate mammalian gene expression, and these studies have broadened our view of cross-kingdom communication. In the present study, we evaluated miRNA levels in cooked maize-containing chow diets, and found that plant miRNAs were resistant to the harsh cooking conditions to a certain extent. After feeding fresh maize to pigs (7 days), maize-derived miRNAs could be detected in porcine tissues and serum, and the authenticity of these plant miRNAs was confirmed by using oxidization reactions. Furthermore, in vivo and in vitro experiments demonstrated that dietary maize miRNAs could cross the gastrointestinal tract and enter the porcine bloodstream. In the porcine cells, we found that plant miRNAs are very likely to specifically target their endogenous porcine mRNAs and influence gene expression in a fashion similar to that of mammalian miRNAs. Our results indicate that maize-derived miRNAs can cross the gastrointestinal tract and present in pigs, and these exogenous miRNAs have the potential to regulate mammalian gene expression. Nature Publishing Group UK 2017-04-05 /pmc/articles/PMC5428504/ /pubmed/28381865 http://dx.doi.org/10.1038/s41598-017-00488-y Text en © The Author(s) 2017 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Luo, Yi
Wang, Pengjun
Wang, Xun
Wang, Yuhao
Mu, Zhiping
Li, Qingzhi
Fu, Yuhua
Xiao, Juan
Li, Guojun
Ma, Yao
Gu, Yiren
Jin, Long
Ma, Jideng
Tang, Qianzi
Jiang, Anan
Li, Xuewei
Li, Mingzhou
Detection of dietetically absorbed maize-derived microRNAs in pigs
title Detection of dietetically absorbed maize-derived microRNAs in pigs
title_full Detection of dietetically absorbed maize-derived microRNAs in pigs
title_fullStr Detection of dietetically absorbed maize-derived microRNAs in pigs
title_full_unstemmed Detection of dietetically absorbed maize-derived microRNAs in pigs
title_short Detection of dietetically absorbed maize-derived microRNAs in pigs
title_sort detection of dietetically absorbed maize-derived micrornas in pigs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428504/
https://www.ncbi.nlm.nih.gov/pubmed/28381865
http://dx.doi.org/10.1038/s41598-017-00488-y
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