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Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis
Kashin-Beck disease (KBD) is a chronic osteochondropathy with unclear pathogeny. In this study, we compared the microRNA expression profiles of 16 KBD patients, 16 osteoarthritis (OA) patients and 16 rheumatoid arthritis (RA) patients and 16 healthy controls in their blood specimens. miRNAs expressi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428653/ https://www.ncbi.nlm.nih.gov/pubmed/28373711 http://dx.doi.org/10.1038/s41598-017-00522-z |
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author | Wu, Wenhong He, Awen Wen, Yan Xiao, Xiao Hao, Jingcan Zhang, Feng Guo, Xiong |
author_facet | Wu, Wenhong He, Awen Wen, Yan Xiao, Xiao Hao, Jingcan Zhang, Feng Guo, Xiong |
author_sort | Wu, Wenhong |
collection | PubMed |
description | Kashin-Beck disease (KBD) is a chronic osteochondropathy with unclear pathogeny. In this study, we compared the microRNA expression profiles of 16 KBD patients, 16 osteoarthritis (OA) patients and 16 rheumatoid arthritis (RA) patients and 16 healthy controls in their blood specimens. miRNAs expression profiling was performed using Exiqon miRCURY LNATM miRNAs Array. miRNAs target genes were predicted using miRror suite. Another independent mRNA expression profile dataset of 20 KBD patients and 15 healthy controls were integrated with the miRNA expression profiles of KBD. We identified 140 differently expressed miRNAs in KBD vs. Controls. GO enrichment analysis found that hypoxia, Wnt receptor signaling pathway and vitamin B6 biosynthesis related GO terms were significantly overrepresented in the target genes of differently expressed miRNAs in KBD vs. Control. 18 differently expressed common miRNAs were identified in KBD vs. Control, KBD vs. OA and KBD vs. RA. Integrating the lists of differently expressed miRNA target genes and mRNA differently expressed genes detected 6 common genes for KBD. Our results demonstrated the altered miRNAs expression profiles of KBD comparing to healthy controls, OA and RA, which provide novel clues for clarifying the mechanism of KBD. |
format | Online Article Text |
id | pubmed-5428653 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54286532017-05-15 Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis Wu, Wenhong He, Awen Wen, Yan Xiao, Xiao Hao, Jingcan Zhang, Feng Guo, Xiong Sci Rep Article Kashin-Beck disease (KBD) is a chronic osteochondropathy with unclear pathogeny. In this study, we compared the microRNA expression profiles of 16 KBD patients, 16 osteoarthritis (OA) patients and 16 rheumatoid arthritis (RA) patients and 16 healthy controls in their blood specimens. miRNAs expression profiling was performed using Exiqon miRCURY LNATM miRNAs Array. miRNAs target genes were predicted using miRror suite. Another independent mRNA expression profile dataset of 20 KBD patients and 15 healthy controls were integrated with the miRNA expression profiles of KBD. We identified 140 differently expressed miRNAs in KBD vs. Controls. GO enrichment analysis found that hypoxia, Wnt receptor signaling pathway and vitamin B6 biosynthesis related GO terms were significantly overrepresented in the target genes of differently expressed miRNAs in KBD vs. Control. 18 differently expressed common miRNAs were identified in KBD vs. Control, KBD vs. OA and KBD vs. RA. Integrating the lists of differently expressed miRNA target genes and mRNA differently expressed genes detected 6 common genes for KBD. Our results demonstrated the altered miRNAs expression profiles of KBD comparing to healthy controls, OA and RA, which provide novel clues for clarifying the mechanism of KBD. Nature Publishing Group UK 2017-04-03 /pmc/articles/PMC5428653/ /pubmed/28373711 http://dx.doi.org/10.1038/s41598-017-00522-z Text en © The Author(s) 2017 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Wu, Wenhong He, Awen Wen, Yan Xiao, Xiao Hao, Jingcan Zhang, Feng Guo, Xiong Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis |
title | Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis |
title_full | Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis |
title_fullStr | Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis |
title_full_unstemmed | Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis |
title_short | Comparison of microRNA expression profiles of Kashin-Beck disease, osteoarthritis and rheumatoid arthritis |
title_sort | comparison of microrna expression profiles of kashin-beck disease, osteoarthritis and rheumatoid arthritis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428653/ https://www.ncbi.nlm.nih.gov/pubmed/28373711 http://dx.doi.org/10.1038/s41598-017-00522-z |
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