Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons

Local mRNA translation occurs in growing axons enabling precise control of the proteome in response to signals. To measure quantitatively the spatiotemporal dynamics of protein synthesis in growth cones, we further developed a technique for single molecule translation imaging (SMTI). We report that...

Descripción completa

Detalles Bibliográficos
Autores principales: Ströhl, Florian, Lin, Julie Qiaojin, Laine, Romain F., Wong, Hovy Ho-Wai, Urbančič, Vasja, Cagnetta, Roberta, Holt, Christine E., Kaminski, Clemens F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428787/
https://www.ncbi.nlm.nih.gov/pubmed/28386060
http://dx.doi.org/10.1038/s41598-017-00695-7
_version_ 1783235901401333760
author Ströhl, Florian
Lin, Julie Qiaojin
Laine, Romain F.
Wong, Hovy Ho-Wai
Urbančič, Vasja
Cagnetta, Roberta
Holt, Christine E.
Kaminski, Clemens F.
author_facet Ströhl, Florian
Lin, Julie Qiaojin
Laine, Romain F.
Wong, Hovy Ho-Wai
Urbančič, Vasja
Cagnetta, Roberta
Holt, Christine E.
Kaminski, Clemens F.
author_sort Ströhl, Florian
collection PubMed
description Local mRNA translation occurs in growing axons enabling precise control of the proteome in response to signals. To measure quantitatively the spatiotemporal dynamics of protein synthesis in growth cones, we further developed a technique for single molecule translation imaging (SMTI). We report that Netrin-1 triggers a burst of β-actin synthesis at multiple non-repetitive sites, particularly in the periphery. The response is remarkably rapid starting within 20 seconds of cue application.
format Online
Article
Text
id pubmed-5428787
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-54287872017-05-15 Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons Ströhl, Florian Lin, Julie Qiaojin Laine, Romain F. Wong, Hovy Ho-Wai Urbančič, Vasja Cagnetta, Roberta Holt, Christine E. Kaminski, Clemens F. Sci Rep Article Local mRNA translation occurs in growing axons enabling precise control of the proteome in response to signals. To measure quantitatively the spatiotemporal dynamics of protein synthesis in growth cones, we further developed a technique for single molecule translation imaging (SMTI). We report that Netrin-1 triggers a burst of β-actin synthesis at multiple non-repetitive sites, particularly in the periphery. The response is remarkably rapid starting within 20 seconds of cue application. Nature Publishing Group UK 2017-04-06 /pmc/articles/PMC5428787/ /pubmed/28386060 http://dx.doi.org/10.1038/s41598-017-00695-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ströhl, Florian
Lin, Julie Qiaojin
Laine, Romain F.
Wong, Hovy Ho-Wai
Urbančič, Vasja
Cagnetta, Roberta
Holt, Christine E.
Kaminski, Clemens F.
Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons
title Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons
title_full Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons
title_fullStr Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons
title_full_unstemmed Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons
title_short Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons
title_sort single molecule translation imaging visualizes the dynamics of local β-actin synthesis in retinal axons
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428787/
https://www.ncbi.nlm.nih.gov/pubmed/28386060
http://dx.doi.org/10.1038/s41598-017-00695-7
work_keys_str_mv AT strohlflorian singlemoleculetranslationimagingvisualizesthedynamicsoflocalbactinsynthesisinretinalaxons
AT linjulieqiaojin singlemoleculetranslationimagingvisualizesthedynamicsoflocalbactinsynthesisinretinalaxons
AT laineromainf singlemoleculetranslationimagingvisualizesthedynamicsoflocalbactinsynthesisinretinalaxons
AT wonghovyhowai singlemoleculetranslationimagingvisualizesthedynamicsoflocalbactinsynthesisinretinalaxons
AT urbancicvasja singlemoleculetranslationimagingvisualizesthedynamicsoflocalbactinsynthesisinretinalaxons
AT cagnettaroberta singlemoleculetranslationimagingvisualizesthedynamicsoflocalbactinsynthesisinretinalaxons
AT holtchristinee singlemoleculetranslationimagingvisualizesthedynamicsoflocalbactinsynthesisinretinalaxons
AT kaminskiclemensf singlemoleculetranslationimagingvisualizesthedynamicsoflocalbactinsynthesisinretinalaxons

Ejemplares similares