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Gene expression profile in human induced pluripotent stem cells: Chondrogenic differentiation in vitro, part B

The development of human induced pluripotent stem cells (hiPSCs) is considered a turning point in tissue engineering. However, more data are required to improve understanding of key aspects of the cell differentiation process, including how specific chondrogenic processes affect the gene expression...

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Autores principales: Augustyniak, Ewelina, Suchorska, Wiktoria Maria, Trzeciak, Tomasz, Richter, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428858/
https://www.ncbi.nlm.nih.gov/pubmed/28447733
http://dx.doi.org/10.3892/mmr.2017.6335
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author Augustyniak, Ewelina
Suchorska, Wiktoria Maria
Trzeciak, Tomasz
Richter, Magdalena
author_facet Augustyniak, Ewelina
Suchorska, Wiktoria Maria
Trzeciak, Tomasz
Richter, Magdalena
author_sort Augustyniak, Ewelina
collection PubMed
description The development of human induced pluripotent stem cells (hiPSCs) is considered a turning point in tissue engineering. However, more data are required to improve understanding of key aspects of the cell differentiation process, including how specific chondrogenic processes affect the gene expression profile of chondrocyte-like cells and the relative value of cell differentiation markers. The main aims of the present study were as follows: To determine the gene expression profile of chondrogenic-like cells derived from hiPSCs cultured in mediums conditioned with HC-402-05a cells or supplemented with transforming growth factor β3 (TGF-β3), and to assess the relative utility of the most commonly-used chondrogenic markers as indicators of cell differentiation. These issues are relevant with regard to the use of human fibroblasts in the reprogramming process to obtain hiPSCs. Human fibroblasts are derived from mesoderm and thus share a wide range of properties with chondrocytes, which originate from the mesenchyme. The hiPSCs were obtained from human primary dermal fibroblasts during a reprogramming process. Two methods, both involving embryoid bodies (EB), were used to obtain chondrocytes from the hiPSCs: EBs formed in the presence of a chondrogenic medium with TGF-β3 (10 ng/ml) and EBs formed in a medium conditioned with growth factors from HC-402-05a cells. Based on reverse transcription-quantitative polymerase chain reaction analysis, the results demonstrated that hiPSCs are capable of effective chondrogenic differentiation, with the cells obtained in the HC-402-05a medium presenting with morphological features and markers characteristic of mature human chondrocytes. In contrast, cells differentiated in the presence of TGF-β3 presented with certain undesirable hypertrophic characteristics. Several genes, most notably runt-related transcription factor 2, transforming growth factor β2 and transforming growth factor β3, were good markers of advanced and late hiPSC chondrogenic differentiation, whereas transforming growth factor β3I, II, III receptors and bone morphogenetic protein-2, bone morphogenetic protein-4 and growth differentiation factor 5 were less valuable. These findings provide valuable data on the use of stem cells in cartilage tissue regeneration.
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spelling pubmed-54288582017-05-15 Gene expression profile in human induced pluripotent stem cells: Chondrogenic differentiation in vitro, part B Augustyniak, Ewelina Suchorska, Wiktoria Maria Trzeciak, Tomasz Richter, Magdalena Mol Med Rep Articles The development of human induced pluripotent stem cells (hiPSCs) is considered a turning point in tissue engineering. However, more data are required to improve understanding of key aspects of the cell differentiation process, including how specific chondrogenic processes affect the gene expression profile of chondrocyte-like cells and the relative value of cell differentiation markers. The main aims of the present study were as follows: To determine the gene expression profile of chondrogenic-like cells derived from hiPSCs cultured in mediums conditioned with HC-402-05a cells or supplemented with transforming growth factor β3 (TGF-β3), and to assess the relative utility of the most commonly-used chondrogenic markers as indicators of cell differentiation. These issues are relevant with regard to the use of human fibroblasts in the reprogramming process to obtain hiPSCs. Human fibroblasts are derived from mesoderm and thus share a wide range of properties with chondrocytes, which originate from the mesenchyme. The hiPSCs were obtained from human primary dermal fibroblasts during a reprogramming process. Two methods, both involving embryoid bodies (EB), were used to obtain chondrocytes from the hiPSCs: EBs formed in the presence of a chondrogenic medium with TGF-β3 (10 ng/ml) and EBs formed in a medium conditioned with growth factors from HC-402-05a cells. Based on reverse transcription-quantitative polymerase chain reaction analysis, the results demonstrated that hiPSCs are capable of effective chondrogenic differentiation, with the cells obtained in the HC-402-05a medium presenting with morphological features and markers characteristic of mature human chondrocytes. In contrast, cells differentiated in the presence of TGF-β3 presented with certain undesirable hypertrophic characteristics. Several genes, most notably runt-related transcription factor 2, transforming growth factor β2 and transforming growth factor β3, were good markers of advanced and late hiPSC chondrogenic differentiation, whereas transforming growth factor β3I, II, III receptors and bone morphogenetic protein-2, bone morphogenetic protein-4 and growth differentiation factor 5 were less valuable. These findings provide valuable data on the use of stem cells in cartilage tissue regeneration. D.A. Spandidos 2017-05 2017-03-16 /pmc/articles/PMC5428858/ /pubmed/28447733 http://dx.doi.org/10.3892/mmr.2017.6335 Text en Copyright: © Augustyniak et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Augustyniak, Ewelina
Suchorska, Wiktoria Maria
Trzeciak, Tomasz
Richter, Magdalena
Gene expression profile in human induced pluripotent stem cells: Chondrogenic differentiation in vitro, part B
title Gene expression profile in human induced pluripotent stem cells: Chondrogenic differentiation in vitro, part B
title_full Gene expression profile in human induced pluripotent stem cells: Chondrogenic differentiation in vitro, part B
title_fullStr Gene expression profile in human induced pluripotent stem cells: Chondrogenic differentiation in vitro, part B
title_full_unstemmed Gene expression profile in human induced pluripotent stem cells: Chondrogenic differentiation in vitro, part B
title_short Gene expression profile in human induced pluripotent stem cells: Chondrogenic differentiation in vitro, part B
title_sort gene expression profile in human induced pluripotent stem cells: chondrogenic differentiation in vitro, part b
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5428858/
https://www.ncbi.nlm.nih.gov/pubmed/28447733
http://dx.doi.org/10.3892/mmr.2017.6335
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