Cargando…

Fast iodide-SAD phasing for high-throughput membrane protein structure determination

We describe a fast, easy, and potentially universal method for the de novo solution of the crystal structures of membrane proteins via iodide–single-wavelength anomalous diffraction (I-SAD). The potential universality of the method is based on a common feature of membrane proteins—the availability a...

Descripción completa

Detalles Bibliográficos
Autores principales:	Melnikov, Igor, Polovinkin, Vitaly, Kovalev, Kirill, Gushchin, Ivan, Shevtsov, Mikhail, Shevchenko, Vitaly, Mishin, Alexey, Alekseev, Alexey, Rodriguez-Valera, Francisco, Borshchevskiy, Valentin, Cherezov, Vadim, Leonard, Gordon A., Gordeliy, Valentin, Popov, Alexander
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Association for the Advancement of Science 2017
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429034/ https://www.ncbi.nlm.nih.gov/pubmed/28508075 http://dx.doi.org/10.1126/sciadv.1602952

Ejemplares similares

Small-wedge synchrotron and serial XFEL datasets for Cysteinyl leukotriene GPCRs
por: Marin, Egor, et al.
Publicado: (2020)

Author Correction: Small-wedge synchrotron and serial XFEL datasets for Cysteinyl leukotriene GPCRs
por: Marin, Egor, et al.
Publicado: (2020)

Structural and Functional Investigation of Flavin Binding Center of the NqrC Subunit of Sodium-Translocating NADH:Quinone Oxidoreductase from Vibrio harveyi
por: Borshchevskiy, Valentin, et al.
Publicado: (2015)

Structure and mechanisms of sodium-pumping KR2 rhodopsin
por: Kovalev, Kirill, et al.
Publicado: (2019)

Inward H(+) pump xenorhodopsin: Mechanism and alternative optogenetic approach
por: Shevchenko, Vitaly, et al.
Publicado: (2017)

An Approach to Heterologous Expression of Membrane Proteins. The Case of Bacteriorhodopsin
por: Bratanov, Dmitry, et al.
Publicado: (2015)

Crystal Structure of Escherichia coli-Expressed Haloarcula marismortui Bacteriorhodopsin I in the Trimeric Form
por: Shevchenko, Vitaly, et al.
Publicado: (2014)

Sensor Histidine Kinase NarQ Activates via Helical Rotation, Diagonal Scissoring, and Eventually Piston-Like Shifts
por: Gushchin, Ivan, et al.
Publicado: (2020)

Structure-based insights into evolution of rhodopsins
por: Zabelskii, Dmitrii, et al.
Publicado: (2021)

Unique structure and function of viral rhodopsins
por: Bratanov, Dmitry, et al.
Publicado: (2019)

The Voltage Dependent Sidedness of the Reprotonation of the Retinal Schiff Base Determines the Unique Inward Pumping of Xenorhodopsin
por: Weissbecker, Juliane, et al.
Publicado: (2021)

Structural basis of ligand selectivity and disease mutations in cysteinyl leukotriene receptors
por: Gusach, Anastasiia, et al.
Publicado: (2019)

Structure-based mechanism of cysteinyl leukotriene receptor inhibition by antiasthmatic drugs
por: Luginina, Aleksandra, et al.
Publicado: (2019)

Flotillin-associated rhodopsin (FArhodopsin), a widespread paralog of proteorhodopsin in aquatic bacteria with streamlined genomes
por: Haro-Moreno, Jose M., et al.
Publicado: (2023)

Structural basis for receptor selectivity and inverse agonism in S1P(5) receptors
por: Lyapina, Elizaveta, et al.
Publicado: (2022)

Structural diversity of leukotriene G-protein coupled receptors
por: Luginina, Aleksandra, et al.
Publicado: (2023)

Efficient non-cytotoxic fluorescent staining of halophiles
por: Maslov, Ivan, et al.
Publicado: (2018)

Sub-millisecond conformational dynamics of the A(2A) adenosine receptor revealed by single-molecule FRET
por: Maslov, Ivan, et al.
Publicado: (2023)

Structural insights into 3Fe–4S ferredoxins diversity in M. tuberculosis highlighted by a first redox complex with P450
por: Gilep, Andrei, et al.
Publicado: (2023)

Structural insights into thrombolytic activity of destabilase from medicinal leech
por: Marin, Egor, et al.
Publicado: (2023)

Crystal structure of semi-synthetic obelin-v after calcium induced bioluminescence implies coelenteramine as the main reaction product
por: Natashin, Pavel V., et al.
Publicado: (2022)

SAD phasing using iodide ions in a high-throughput structural genomics environment
por: Abendroth, Jan, et al.
Publicado: (2011)

High-pressure crystallography shows noble gas intervention into protein-lipid interaction and suggests a model for anaesthetic action
por: Melnikov, Igor, et al.
Publicado: (2022)

Murine Intraepithelial Dendritic Cells Interact With Phagocytic Cells During Aspergillus fumigatus-Induced Inflammation
por: Bogorodskiy, Andrey O., et al.
Publicado: (2020)

Molecular mechanism of light-driven sodium pumping
por: Kovalev, Kirill, et al.
Publicado: (2020)

On the Origin of the Anomalous Behavior of Lipid Membrane Properties in the Vicinity of the Chain-Melting Phase Transition
por: Kuklin, Alexander, et al.
Publicado: (2020)

High-resolution structural insights into the heliorhodopsin family
por: Kovalev, K., et al.
Publicado: (2020)

Structure-Based Virtual Screening of Ultra-Large Library Yields Potent Antagonists for a Lipid GPCR
por: Sadybekov, Arman A., et al.
Publicado: (2020)

The molecular basis of spectral tuning in blue- and red-shifted flavin-binding fluorescent proteins
por: Röllen, Katrin, et al.
Publicado: (2021)

Accessing Mitochondrial Protein Import in Living Cells by Protein Microinjection
por: Bogorodskiy, Andrey, et al.
Publicado: (2021)

NanoFAST: structure-based design of a small fluorogen-activating protein with only 98 amino acids
por: Mineev, Konstantin S., et al.
Publicado: (2021)

New Insights on Signal Propagation by Sensory Rhodopsin II/Transducer Complex
por: Ishchenko, A., et al.
Publicado: (2017)

The Role of Tyr-His-Trp Triad and Water Molecule Near the N1-Atom of 2-Hydroperoxycoelenterazine in Bioluminescence of Hydromedusan Photoproteins: Structural and Mutagenesis Study
por: Natashin, Pavel V., et al.
Publicado: (2023)

Two Distinct States of the HAMP Domain from Sensory Rhodopsin Transducer Observed in Unbiased Molecular Dynamics Simulations
por: Gushchin, Ivan, et al.
Publicado: (2013)

Structure-based rational design of an enhanced fluorogen-activating protein for fluorogens based on GFP chromophore
por: Goncharuk, Marina V., et al.
Publicado: (2022)

Unusual features of the c-ring of F(1)F(O) ATP synthases
por: Vlasov, A. V., et al.
Publicado: (2019)

Aspergillus fumigatus Infection-Induced Neutrophil Recruitment and Location in the Conducting Airway of Immunocompetent, Neutropenic, and Immunosuppressed Mice
por: Shevchenko, Marina A., et al.
Publicado: (2018)

A Sad, Sad Case
Publicado: (1861)

Mirror proteorhodopsins
por: Okhrimenko, Ivan S., et al.
Publicado: (2023)

ML-Based Analysis of Particle Distributions in High-Intensity Laser Experiments: Role of Binning Strategy
por: Rodimkov, Yury, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_dmkqqbsk1kkjpubp2gpltqcu8o