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Bioflocculation potentials of a uronic acid-containing glycoprotein produced by Bacillus sp. AEMREG4 isolated from Tyhume River, South Africa

Bioflocculants are secondary metabolites produced by microorganisms during their growth which have received attentions due to their biodegradability, innocuousness and lack of secondary pollution from degradation intermediates. This study reports on a bioflocculant produced by Bacillus specie isolat...

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Autores principales: Ntsangani, Nozipho, Okaiyeto, Kunle, Uchechukwu, Nwodo U., Olaniran, Ademola O., Mabinya, Leonard V., Okoh, Anthony I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429313/
https://www.ncbi.nlm.nih.gov/pubmed/28500400
http://dx.doi.org/10.1007/s13205-017-0695-8
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author Ntsangani, Nozipho
Okaiyeto, Kunle
Uchechukwu, Nwodo U.
Olaniran, Ademola O.
Mabinya, Leonard V.
Okoh, Anthony I.
author_facet Ntsangani, Nozipho
Okaiyeto, Kunle
Uchechukwu, Nwodo U.
Olaniran, Ademola O.
Mabinya, Leonard V.
Okoh, Anthony I.
author_sort Ntsangani, Nozipho
collection PubMed
description Bioflocculants are secondary metabolites produced by microorganisms during their growth which have received attentions due to their biodegradability, innocuousness and lack of secondary pollution from degradation intermediates. This study reports on a bioflocculant produced by Bacillus specie isolated from Thyume River in South Africa. The bacterial isolate was identified through 16S rDNA sequencing and the BLAST analysis of the nucleotide sequences revealed 99% similarity to Bacillus sp. BCT-7112. The sequence was subsequently deposited in the GenBank as Bacillus sp. AEMREG4 with accession number KP406729. The optimum culture conditions for bioflocculant production were an inoculum size 4% (v/v) (80%) and starch (81%) as well as yeast extract (82%) as sole carbon and nitrogen sources, respectively. Addition of Ca(2+) greatly enhanced the flocculating activity (76%) of crude bioflocculant over a wide range of pH 4–10 and retained high flocculating activity when heated at 100 °C for 1 h. Chemical analyses of the purified bioflocculant revealed carbohydrate (79% w/w) as a predominant component followed by uronic acid (15% w/w) and protein (5% w/w). Fourier transform infrared spectrum revealed the presence of carboxyl, hydroxyl and methoxyl groups as the functional groups responsible for flocculation and the high flocculation activity achieved portends its industrial applicability.
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spelling pubmed-54293132017-05-30 Bioflocculation potentials of a uronic acid-containing glycoprotein produced by Bacillus sp. AEMREG4 isolated from Tyhume River, South Africa Ntsangani, Nozipho Okaiyeto, Kunle Uchechukwu, Nwodo U. Olaniran, Ademola O. Mabinya, Leonard V. Okoh, Anthony I. 3 Biotech Original Article Bioflocculants are secondary metabolites produced by microorganisms during their growth which have received attentions due to their biodegradability, innocuousness and lack of secondary pollution from degradation intermediates. This study reports on a bioflocculant produced by Bacillus specie isolated from Thyume River in South Africa. The bacterial isolate was identified through 16S rDNA sequencing and the BLAST analysis of the nucleotide sequences revealed 99% similarity to Bacillus sp. BCT-7112. The sequence was subsequently deposited in the GenBank as Bacillus sp. AEMREG4 with accession number KP406729. The optimum culture conditions for bioflocculant production were an inoculum size 4% (v/v) (80%) and starch (81%) as well as yeast extract (82%) as sole carbon and nitrogen sources, respectively. Addition of Ca(2+) greatly enhanced the flocculating activity (76%) of crude bioflocculant over a wide range of pH 4–10 and retained high flocculating activity when heated at 100 °C for 1 h. Chemical analyses of the purified bioflocculant revealed carbohydrate (79% w/w) as a predominant component followed by uronic acid (15% w/w) and protein (5% w/w). Fourier transform infrared spectrum revealed the presence of carboxyl, hydroxyl and methoxyl groups as the functional groups responsible for flocculation and the high flocculation activity achieved portends its industrial applicability. Springer Berlin Heidelberg 2017-05-12 2017-05 /pmc/articles/PMC5429313/ /pubmed/28500400 http://dx.doi.org/10.1007/s13205-017-0695-8 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Ntsangani, Nozipho
Okaiyeto, Kunle
Uchechukwu, Nwodo U.
Olaniran, Ademola O.
Mabinya, Leonard V.
Okoh, Anthony I.
Bioflocculation potentials of a uronic acid-containing glycoprotein produced by Bacillus sp. AEMREG4 isolated from Tyhume River, South Africa
title Bioflocculation potentials of a uronic acid-containing glycoprotein produced by Bacillus sp. AEMREG4 isolated from Tyhume River, South Africa
title_full Bioflocculation potentials of a uronic acid-containing glycoprotein produced by Bacillus sp. AEMREG4 isolated from Tyhume River, South Africa
title_fullStr Bioflocculation potentials of a uronic acid-containing glycoprotein produced by Bacillus sp. AEMREG4 isolated from Tyhume River, South Africa
title_full_unstemmed Bioflocculation potentials of a uronic acid-containing glycoprotein produced by Bacillus sp. AEMREG4 isolated from Tyhume River, South Africa
title_short Bioflocculation potentials of a uronic acid-containing glycoprotein produced by Bacillus sp. AEMREG4 isolated from Tyhume River, South Africa
title_sort bioflocculation potentials of a uronic acid-containing glycoprotein produced by bacillus sp. aemreg4 isolated from tyhume river, south africa
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429313/
https://www.ncbi.nlm.nih.gov/pubmed/28500400
http://dx.doi.org/10.1007/s13205-017-0695-8
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