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Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum
BACKGROUND: Cost-efficient saccharification is one of the main bottlenecks for industrial lignocellulose conversion. Clostridium thermocellum naturally degrades lignocellulose efficiently using the cellulosome, a multiprotein supermolecular complex, and thus can be potentially used as a low-cost cat...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429504/ https://www.ncbi.nlm.nih.gov/pubmed/28507596 http://dx.doi.org/10.1186/s13068-017-0796-y |
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author | Zhang, Jie Liu, Shiyue Li, Renmin Hong, Wei Xiao, Yan Feng, Yingang Cui, Qiu Liu, Ya-Jun |
author_facet | Zhang, Jie Liu, Shiyue Li, Renmin Hong, Wei Xiao, Yan Feng, Yingang Cui, Qiu Liu, Ya-Jun |
author_sort | Zhang, Jie |
collection | PubMed |
description | BACKGROUND: Cost-efficient saccharification is one of the main bottlenecks for industrial lignocellulose conversion. Clostridium thermocellum naturally degrades lignocellulose efficiently using the cellulosome, a multiprotein supermolecular complex, and thus can be potentially used as a low-cost catalyst for lignocellulose saccharification. The industrial use of C. thermocellum is restrained due largely to the inhibition of the hydrolysate cellobiose to its cellulosome. Although the supplementation of beta-glucosidase may solve the problem, the production of the enzymes greatly complicates the process and may also increase the cost of saccharification. RESULTS: To conquer the feedback inhibition and establish an efficient whole-cell catalyst for highly efficient cellulose saccharification, we constructed a recombinant strain of C. thermocellum ∆pyrF::CaBglA which produced a secretory exoglucanase CelS-bearing heterologous BGL using a newly developed seamless genome editing system. Without the extra addition of enzymes, the relative saccharification level of ∆pyrF::CaBglA was stimulated by over twofolds compared to its parent strain ∆pyrF through a two-stage saccharification process with 100 g/L Avicel as the carbon source. The production of reducing sugars and the relative saccharification level were further enhanced to 490 mM and 79.4%, respectively, with increased cell density. CONCLUSIONS: The high cellulose-degrading ability and sugar productivity suggested that the whole-cell-catalysis strategy for cellulose saccharification is promising, and the C. thermocellum strain ∆pyrF::CaBglA could be potentially used as an efficient whole-cell catalyst for industrial cellulose saccharification. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13068-017-0796-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5429504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54295042017-05-15 Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum Zhang, Jie Liu, Shiyue Li, Renmin Hong, Wei Xiao, Yan Feng, Yingang Cui, Qiu Liu, Ya-Jun Biotechnol Biofuels Research BACKGROUND: Cost-efficient saccharification is one of the main bottlenecks for industrial lignocellulose conversion. Clostridium thermocellum naturally degrades lignocellulose efficiently using the cellulosome, a multiprotein supermolecular complex, and thus can be potentially used as a low-cost catalyst for lignocellulose saccharification. The industrial use of C. thermocellum is restrained due largely to the inhibition of the hydrolysate cellobiose to its cellulosome. Although the supplementation of beta-glucosidase may solve the problem, the production of the enzymes greatly complicates the process and may also increase the cost of saccharification. RESULTS: To conquer the feedback inhibition and establish an efficient whole-cell catalyst for highly efficient cellulose saccharification, we constructed a recombinant strain of C. thermocellum ∆pyrF::CaBglA which produced a secretory exoglucanase CelS-bearing heterologous BGL using a newly developed seamless genome editing system. Without the extra addition of enzymes, the relative saccharification level of ∆pyrF::CaBglA was stimulated by over twofolds compared to its parent strain ∆pyrF through a two-stage saccharification process with 100 g/L Avicel as the carbon source. The production of reducing sugars and the relative saccharification level were further enhanced to 490 mM and 79.4%, respectively, with increased cell density. CONCLUSIONS: The high cellulose-degrading ability and sugar productivity suggested that the whole-cell-catalysis strategy for cellulose saccharification is promising, and the C. thermocellum strain ∆pyrF::CaBglA could be potentially used as an efficient whole-cell catalyst for industrial cellulose saccharification. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13068-017-0796-y) contains supplementary material, which is available to authorized users. BioMed Central 2017-05-12 /pmc/articles/PMC5429504/ /pubmed/28507596 http://dx.doi.org/10.1186/s13068-017-0796-y Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Zhang, Jie Liu, Shiyue Li, Renmin Hong, Wei Xiao, Yan Feng, Yingang Cui, Qiu Liu, Ya-Jun Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum |
title | Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum |
title_full | Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum |
title_fullStr | Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum |
title_full_unstemmed | Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum |
title_short | Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum |
title_sort | efficient whole-cell-catalyzing cellulose saccharification using engineered clostridium thermocellum |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429504/ https://www.ncbi.nlm.nih.gov/pubmed/28507596 http://dx.doi.org/10.1186/s13068-017-0796-y |
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