Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy

The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human smal...

Descripción completa

Detalles Bibliográficos
Autores principales: Veres, Tibor Z., Kopcsányi, Tamás, Tirri, Marko, Braun, Armin, Miyasaka, Masayuki, Germain, Ronald N., Jalkanen, Sirpa, Salmi, Marko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429620/
https://www.ncbi.nlm.nih.gov/pubmed/28386104
http://dx.doi.org/10.1038/s41598-017-00769-6
_version_ 1783236060400058368
author Veres, Tibor Z.
Kopcsányi, Tamás
Tirri, Marko
Braun, Armin
Miyasaka, Masayuki
Germain, Ronald N.
Jalkanen, Sirpa
Salmi, Marko
author_facet Veres, Tibor Z.
Kopcsányi, Tamás
Tirri, Marko
Braun, Armin
Miyasaka, Masayuki
Germain, Ronald N.
Jalkanen, Sirpa
Salmi, Marko
author_sort Veres, Tibor Z.
collection PubMed
description The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell behavior within the complex airway mucosal tissue.
format Online
Article
Text
id pubmed-5429620
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-54296202017-05-15 Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy Veres, Tibor Z. Kopcsányi, Tamás Tirri, Marko Braun, Armin Miyasaka, Masayuki Germain, Ronald N. Jalkanen, Sirpa Salmi, Marko Sci Rep Article The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell behavior within the complex airway mucosal tissue. Nature Publishing Group UK 2017-04-06 /pmc/articles/PMC5429620/ /pubmed/28386104 http://dx.doi.org/10.1038/s41598-017-00769-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Veres, Tibor Z.
Kopcsányi, Tamás
Tirri, Marko
Braun, Armin
Miyasaka, Masayuki
Germain, Ronald N.
Jalkanen, Sirpa
Salmi, Marko
Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_full Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_fullStr Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_full_unstemmed Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_short Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_sort intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429620/
https://www.ncbi.nlm.nih.gov/pubmed/28386104
http://dx.doi.org/10.1038/s41598-017-00769-6
work_keys_str_mv AT verestiborz intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
AT kopcsanyitamas intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
AT tirrimarko intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
AT braunarmin intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
AT miyasakamasayuki intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
AT germainronaldn intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
AT jalkanensirpa intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
AT salmimarko intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy

Ejemplares similares