Biosafety Level 3 setup for multiphoton microscopy in vivo

Multiphoton microscopy has revealed important insights into cellular behavior in vivo. However, its application in infectious settings often encounters technical, safety and regulatory limitations that prevent its wider use with highly virulent human pathogens. Herein, we present a method that rende...

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Autores principales: Barlerin, D., Bessière, G., Domingues, J., Schuette, M., Feuillet, C., Peixoto, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429670/
https://www.ncbi.nlm.nih.gov/pubmed/28373723
http://dx.doi.org/10.1038/s41598-017-00702-x
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author Barlerin, D.
Bessière, G.
Domingues, J.
Schuette, M.
Feuillet, C.
Peixoto, A.
author_facet Barlerin, D.
Bessière, G.
Domingues, J.
Schuette, M.
Feuillet, C.
Peixoto, A.
author_sort Barlerin, D.
collection PubMed
description Multiphoton microscopy has revealed important insights into cellular behavior in vivo. However, its application in infectious settings often encounters technical, safety and regulatory limitations that prevent its wider use with highly virulent human pathogens. Herein, we present a method that renders multiphoton microscopy in vivo compatible with biosafety level 3 regulations and present an example of its application and potential to visualize a Mycobacterium tuberculosis infection of the mouse lung.
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spelling pubmed-54296702017-05-15 Biosafety Level 3 setup for multiphoton microscopy in vivo Barlerin, D. Bessière, G. Domingues, J. Schuette, M. Feuillet, C. Peixoto, A. Sci Rep Article Multiphoton microscopy has revealed important insights into cellular behavior in vivo. However, its application in infectious settings often encounters technical, safety and regulatory limitations that prevent its wider use with highly virulent human pathogens. Herein, we present a method that renders multiphoton microscopy in vivo compatible with biosafety level 3 regulations and present an example of its application and potential to visualize a Mycobacterium tuberculosis infection of the mouse lung. Nature Publishing Group UK 2017-04-03 /pmc/articles/PMC5429670/ /pubmed/28373723 http://dx.doi.org/10.1038/s41598-017-00702-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Barlerin, D.
Bessière, G.
Domingues, J.
Schuette, M.
Feuillet, C.
Peixoto, A.
Biosafety Level 3 setup for multiphoton microscopy in vivo
title Biosafety Level 3 setup for multiphoton microscopy in vivo
title_full Biosafety Level 3 setup for multiphoton microscopy in vivo
title_fullStr Biosafety Level 3 setup for multiphoton microscopy in vivo
title_full_unstemmed Biosafety Level 3 setup for multiphoton microscopy in vivo
title_short Biosafety Level 3 setup for multiphoton microscopy in vivo
title_sort biosafety level 3 setup for multiphoton microscopy in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429670/
https://www.ncbi.nlm.nih.gov/pubmed/28373723
http://dx.doi.org/10.1038/s41598-017-00702-x
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