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Nanoplasmonic Upconverting Nanoparticles as Orientation Sensors for Single Particle Microscopy

We showed that the anisotropic disk shape of nanoplasmonic upconverting nanoparticles (NP-UCNPs) creates changes in fluorescence intensity during rotational motion. We determined the orientation by a three-fold change in fluorescence intensity. We further found that the luminescence intensity was st...

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Detalles Bibliográficos
Autores principales: Green, Kory K., Wirth, Janina, Lim, Shuang F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429696/
https://www.ncbi.nlm.nih.gov/pubmed/28396602
http://dx.doi.org/10.1038/s41598-017-00869-3
Descripción
Sumario:We showed that the anisotropic disk shape of nanoplasmonic upconverting nanoparticles (NP-UCNPs) creates changes in fluorescence intensity during rotational motion. We determined the orientation by a three-fold change in fluorescence intensity. We further found that the luminescence intensity was strongly dependent on the particle orientation and on polarization of the excitation light. The luminescence intensity showed a three-fold difference between flat and on-edge orientations. The intensity also varied sinusoidally with the polarization of the incident light, with an I(max)/I(min) ratio of up to 2.02. Both the orientation dependence and I(max)/I(min) are dependent on the presence of a gold shell on the UCNP. Because the fluorescence depends on the NP’s orientation, the rotational motion of biomolecules coupled to the NP can be detected. Finally, we tracked the real-time rotational motion of a single NP-UCNP in solution between slide and coverslip with diffusivity up to 10(−2) μm(2)s(−1).