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Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase
AMP-activated protein kinase (AMPK) is a key sensor and regulator of glucose metabolism. Here, we demonstrated that shizukaol F, a natural compound isolated from Chloranthus japonicus, can activate AMPK and modulate glucose metabolism both in vitro and in vivo. Shizukaol F increased glucose uptake i...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429703/ https://www.ncbi.nlm.nih.gov/pubmed/28396610 http://dx.doi.org/10.1038/s41598-017-00925-y |
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author | Hu, Rongkuan Yan, Huan Fei, Xiaoyan Liu, Haiyang Wu, Jiarui |
author_facet | Hu, Rongkuan Yan, Huan Fei, Xiaoyan Liu, Haiyang Wu, Jiarui |
author_sort | Hu, Rongkuan |
collection | PubMed |
description | AMP-activated protein kinase (AMPK) is a key sensor and regulator of glucose metabolism. Here, we demonstrated that shizukaol F, a natural compound isolated from Chloranthus japonicus, can activate AMPK and modulate glucose metabolism both in vitro and in vivo. Shizukaol F increased glucose uptake in differentiated C2C12 myotubes by stimulating glucose transporter-4 (GLUT-4) membraned translocation. Treatment of primary mouse hepatocytes with shizukaol F decreased the expression of phosphoenolpyruvate carboxykinase 2 (PEPCK), glucose-6-phosphatase (G6Pase) and suppressed hepatic gluconeogenesis. Meanwhile, a single oral dose of shizukaol F reduced gluconeogenesis in C57BL/6 J mice. Further studies indicated that shizukaol F modulates glucose metabolism mainly by AMPKa phosphorylation activity. In addition, we also found that shizukaol F depolarizes the mitochondrial membrane and inhibits respiratory complex I, which may result in AMPK activation. Our results highlight the potential value of shizukaol F as a possible treatment of metabolic syndrome. |
format | Online Article Text |
id | pubmed-5429703 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54297032017-05-15 Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase Hu, Rongkuan Yan, Huan Fei, Xiaoyan Liu, Haiyang Wu, Jiarui Sci Rep Article AMP-activated protein kinase (AMPK) is a key sensor and regulator of glucose metabolism. Here, we demonstrated that shizukaol F, a natural compound isolated from Chloranthus japonicus, can activate AMPK and modulate glucose metabolism both in vitro and in vivo. Shizukaol F increased glucose uptake in differentiated C2C12 myotubes by stimulating glucose transporter-4 (GLUT-4) membraned translocation. Treatment of primary mouse hepatocytes with shizukaol F decreased the expression of phosphoenolpyruvate carboxykinase 2 (PEPCK), glucose-6-phosphatase (G6Pase) and suppressed hepatic gluconeogenesis. Meanwhile, a single oral dose of shizukaol F reduced gluconeogenesis in C57BL/6 J mice. Further studies indicated that shizukaol F modulates glucose metabolism mainly by AMPKa phosphorylation activity. In addition, we also found that shizukaol F depolarizes the mitochondrial membrane and inhibits respiratory complex I, which may result in AMPK activation. Our results highlight the potential value of shizukaol F as a possible treatment of metabolic syndrome. Nature Publishing Group UK 2017-04-10 /pmc/articles/PMC5429703/ /pubmed/28396610 http://dx.doi.org/10.1038/s41598-017-00925-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Hu, Rongkuan Yan, Huan Fei, Xiaoyan Liu, Haiyang Wu, Jiarui Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase |
title | Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase |
title_full | Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase |
title_fullStr | Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase |
title_full_unstemmed | Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase |
title_short | Modulation of glucose metabolism by a natural compound from Chloranthus japonicus via activation of AMP-activated protein kinase |
title_sort | modulation of glucose metabolism by a natural compound from chloranthus japonicus via activation of amp-activated protein kinase |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429703/ https://www.ncbi.nlm.nih.gov/pubmed/28396610 http://dx.doi.org/10.1038/s41598-017-00925-y |
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