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Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy
Raman spectroscopy allows label-free, minimally invasive, and accurate detection of peripheral nerves. However, the conventional Raman imaging technique is time-consuming when measuring a large area of a sample. Establishing a method for rapidly acquiring spatial distribution of a bundle of peripher...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429797/ https://www.ncbi.nlm.nih.gov/pubmed/28405007 http://dx.doi.org/10.1038/s41598-017-00995-y |
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author | Kumamoto, Yasuaki Harada, Yoshinori Tanaka, Hideo Takamatsu, Tetsuro |
author_facet | Kumamoto, Yasuaki Harada, Yoshinori Tanaka, Hideo Takamatsu, Tetsuro |
author_sort | Kumamoto, Yasuaki |
collection | PubMed |
description | Raman spectroscopy allows label-free, minimally invasive, and accurate detection of peripheral nerves. However, the conventional Raman imaging technique is time-consuming when measuring a large area of a sample. Establishing a method for rapidly acquiring spatial distribution of a bundle of peripheral nerve fibers is an essential step for Raman spectroscopy towards application in clinical surgery. Here we present a multipoint Raman spectroscopic technique for rapid peripheral nerve imaging. In only 5 seconds, spectra at 32 points situated on ex vivo rat peripheral nerve bundles and adjoining connective tissues were acquired. Principal component regression and discriminant analysis of spectra revealed that the sensitivity, specificity and accuracy for nerve detection were 85.8%, 96.0%, and 90.8%, respectively. Of 158 peripheral nerves, 152 (96.2%) showed ratio of the number of nerve-positive prediction points to the total measurement points being 0.4 or larger, whereas 119 (99.2%) connective tissues among 120 showed ratio smaller than 0.4. Based on the ratio and a bright-field image of the sample, accurate visualization of peripheral nerves was implemented. The results indicated that the multipoint Raman spectroscopic technique is capable of rapid and accurate peripheral nerve imaging. |
format | Online Article Text |
id | pubmed-5429797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54297972017-05-15 Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy Kumamoto, Yasuaki Harada, Yoshinori Tanaka, Hideo Takamatsu, Tetsuro Sci Rep Article Raman spectroscopy allows label-free, minimally invasive, and accurate detection of peripheral nerves. However, the conventional Raman imaging technique is time-consuming when measuring a large area of a sample. Establishing a method for rapidly acquiring spatial distribution of a bundle of peripheral nerve fibers is an essential step for Raman spectroscopy towards application in clinical surgery. Here we present a multipoint Raman spectroscopic technique for rapid peripheral nerve imaging. In only 5 seconds, spectra at 32 points situated on ex vivo rat peripheral nerve bundles and adjoining connective tissues were acquired. Principal component regression and discriminant analysis of spectra revealed that the sensitivity, specificity and accuracy for nerve detection were 85.8%, 96.0%, and 90.8%, respectively. Of 158 peripheral nerves, 152 (96.2%) showed ratio of the number of nerve-positive prediction points to the total measurement points being 0.4 or larger, whereas 119 (99.2%) connective tissues among 120 showed ratio smaller than 0.4. Based on the ratio and a bright-field image of the sample, accurate visualization of peripheral nerves was implemented. The results indicated that the multipoint Raman spectroscopic technique is capable of rapid and accurate peripheral nerve imaging. Nature Publishing Group UK 2017-04-12 /pmc/articles/PMC5429797/ /pubmed/28405007 http://dx.doi.org/10.1038/s41598-017-00995-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kumamoto, Yasuaki Harada, Yoshinori Tanaka, Hideo Takamatsu, Tetsuro Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy |
title | Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy |
title_full | Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy |
title_fullStr | Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy |
title_full_unstemmed | Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy |
title_short | Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy |
title_sort | rapid and accurate peripheral nerve imaging by multipoint raman spectroscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429797/ https://www.ncbi.nlm.nih.gov/pubmed/28405007 http://dx.doi.org/10.1038/s41598-017-00995-y |
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