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Glucose uptake in Azotobacter vinelandii occurs through a GluP transporter that is under the control of the CbrA/CbrB and Hfq-Crc systems
Azotobacter vinelandii, a strict aerobic, nitrogen fixing bacterium in the Pseudomonadaceae family, exhibits a preferential use of acetate over glucose as a carbon source. In this study, we show that GluP (Avin04150), annotated as an H(+)-coupled glucose-galactose symporter, is the glucose transport...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429807/ https://www.ncbi.nlm.nih.gov/pubmed/28404995 http://dx.doi.org/10.1038/s41598-017-00980-5 |
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author | Quiroz-Rocha, Elva Moreno, Renata Hernández-Ortíz, Armando Fragoso-Jiménez, Juan Carlos Muriel-Millán, Luis Felipe Guzmán, Josefina Espín, Guadalupe Rojo, Fernando Núñez, Cinthia |
author_facet | Quiroz-Rocha, Elva Moreno, Renata Hernández-Ortíz, Armando Fragoso-Jiménez, Juan Carlos Muriel-Millán, Luis Felipe Guzmán, Josefina Espín, Guadalupe Rojo, Fernando Núñez, Cinthia |
author_sort | Quiroz-Rocha, Elva |
collection | PubMed |
description | Azotobacter vinelandii, a strict aerobic, nitrogen fixing bacterium in the Pseudomonadaceae family, exhibits a preferential use of acetate over glucose as a carbon source. In this study, we show that GluP (Avin04150), annotated as an H(+)-coupled glucose-galactose symporter, is the glucose transporter in A. vinelandii. This protein, which is widely distributed in bacteria and archaea, is uncommon in Pseudomonas species. We found that expression of gluP was under catabolite repression control thorugh the CbrA/CbrB and Crc/Hfq regulatory systems, which were functionally conserved between A. vinelandii and Pseudomonas species. While the histidine kinase CbrA was essential for glucose utilization, over-expression of the Crc protein arrested cell growth when glucose was the sole carbon source. Crc and Hfq proteins from either A. vinelandii or P. putida could form a stable complex with an RNA A-rich Hfq-binding motif present in the leader region of gluP mRNA. Moreover, in P. putida, the gluP A-rich Hfq-binding motif was functional and promoted translational inhibition of a lacZ reporter gene. The fact that gluP is not widely distributed in the Pseudomonas genus but is under control of the CbrA/CbrB and Crc/Hfq systems demonstrates the relevance of these systems in regulating metabolism in the Pseudomonadaceae family. |
format | Online Article Text |
id | pubmed-5429807 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54298072017-05-15 Glucose uptake in Azotobacter vinelandii occurs through a GluP transporter that is under the control of the CbrA/CbrB and Hfq-Crc systems Quiroz-Rocha, Elva Moreno, Renata Hernández-Ortíz, Armando Fragoso-Jiménez, Juan Carlos Muriel-Millán, Luis Felipe Guzmán, Josefina Espín, Guadalupe Rojo, Fernando Núñez, Cinthia Sci Rep Article Azotobacter vinelandii, a strict aerobic, nitrogen fixing bacterium in the Pseudomonadaceae family, exhibits a preferential use of acetate over glucose as a carbon source. In this study, we show that GluP (Avin04150), annotated as an H(+)-coupled glucose-galactose symporter, is the glucose transporter in A. vinelandii. This protein, which is widely distributed in bacteria and archaea, is uncommon in Pseudomonas species. We found that expression of gluP was under catabolite repression control thorugh the CbrA/CbrB and Crc/Hfq regulatory systems, which were functionally conserved between A. vinelandii and Pseudomonas species. While the histidine kinase CbrA was essential for glucose utilization, over-expression of the Crc protein arrested cell growth when glucose was the sole carbon source. Crc and Hfq proteins from either A. vinelandii or P. putida could form a stable complex with an RNA A-rich Hfq-binding motif present in the leader region of gluP mRNA. Moreover, in P. putida, the gluP A-rich Hfq-binding motif was functional and promoted translational inhibition of a lacZ reporter gene. The fact that gluP is not widely distributed in the Pseudomonas genus but is under control of the CbrA/CbrB and Crc/Hfq systems demonstrates the relevance of these systems in regulating metabolism in the Pseudomonadaceae family. Nature Publishing Group UK 2017-04-12 /pmc/articles/PMC5429807/ /pubmed/28404995 http://dx.doi.org/10.1038/s41598-017-00980-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Quiroz-Rocha, Elva Moreno, Renata Hernández-Ortíz, Armando Fragoso-Jiménez, Juan Carlos Muriel-Millán, Luis Felipe Guzmán, Josefina Espín, Guadalupe Rojo, Fernando Núñez, Cinthia Glucose uptake in Azotobacter vinelandii occurs through a GluP transporter that is under the control of the CbrA/CbrB and Hfq-Crc systems |
title | Glucose uptake in Azotobacter vinelandii occurs through a GluP transporter that is under the control of the CbrA/CbrB and Hfq-Crc systems |
title_full | Glucose uptake in Azotobacter vinelandii occurs through a GluP transporter that is under the control of the CbrA/CbrB and Hfq-Crc systems |
title_fullStr | Glucose uptake in Azotobacter vinelandii occurs through a GluP transporter that is under the control of the CbrA/CbrB and Hfq-Crc systems |
title_full_unstemmed | Glucose uptake in Azotobacter vinelandii occurs through a GluP transporter that is under the control of the CbrA/CbrB and Hfq-Crc systems |
title_short | Glucose uptake in Azotobacter vinelandii occurs through a GluP transporter that is under the control of the CbrA/CbrB and Hfq-Crc systems |
title_sort | glucose uptake in azotobacter vinelandii occurs through a glup transporter that is under the control of the cbra/cbrb and hfq-crc systems |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429807/ https://www.ncbi.nlm.nih.gov/pubmed/28404995 http://dx.doi.org/10.1038/s41598-017-00980-5 |
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