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Moderate plasma dilution using artificial plasma expanders shifts the haemostatic balance to hypercoagulation

Artificial plasma expanders (PEs) are widely used in modern transfusion medicine. PEs do not contain components of the coagulation system, so their infusion in large volumes causes haemodilution and affects haemostasis. However, the existing information on this effect is contradictory. We studied th...

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Autores principales: Sinauridze, Elena I., Gorbatenko, Alexander S., Seregina, Elena A., Lipets, Elena N., Ataullakhanov, Fazoil I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429808/
https://www.ncbi.nlm.nih.gov/pubmed/28405015
http://dx.doi.org/10.1038/s41598-017-00927-w
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author Sinauridze, Elena I.
Gorbatenko, Alexander S.
Seregina, Elena A.
Lipets, Elena N.
Ataullakhanov, Fazoil I.
author_facet Sinauridze, Elena I.
Gorbatenko, Alexander S.
Seregina, Elena A.
Lipets, Elena N.
Ataullakhanov, Fazoil I.
author_sort Sinauridze, Elena I.
collection PubMed
description Artificial plasma expanders (PEs) are widely used in modern transfusion medicine. PEs do not contain components of the coagulation system, so their infusion in large volumes causes haemodilution and affects haemostasis. However, the existing information on this effect is contradictory. We studied the effect of the very process of plasma dilution on coagulation and tested the hypothesis that moderate dilution with a PE should accelerate clotting owing to a decrease in concentration of coagulation inhibitors. The standard clotting times, a thrombin generation test, and the spatial rate of clot growth (test of thrombodynamics) were used to assess donor plasma diluted in vitro with various PEs. The pH value and Ca(+2) concentration were maintained strictly constant in all samples. The effect of thrombin inhibitors on dilution-induced hypercoagulation was also examined. It was shown that coagulation was enhanced in plasma diluted up to 2.0–2.5-fold with any PE. This enhancement was due to the dilution of coagulation inhibitors in plasma. Their addition to plasma or PE could partially prevent the hypercoagulation shift.
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spelling pubmed-54298082017-05-15 Moderate plasma dilution using artificial plasma expanders shifts the haemostatic balance to hypercoagulation Sinauridze, Elena I. Gorbatenko, Alexander S. Seregina, Elena A. Lipets, Elena N. Ataullakhanov, Fazoil I. Sci Rep Article Artificial plasma expanders (PEs) are widely used in modern transfusion medicine. PEs do not contain components of the coagulation system, so their infusion in large volumes causes haemodilution and affects haemostasis. However, the existing information on this effect is contradictory. We studied the effect of the very process of plasma dilution on coagulation and tested the hypothesis that moderate dilution with a PE should accelerate clotting owing to a decrease in concentration of coagulation inhibitors. The standard clotting times, a thrombin generation test, and the spatial rate of clot growth (test of thrombodynamics) were used to assess donor plasma diluted in vitro with various PEs. The pH value and Ca(+2) concentration were maintained strictly constant in all samples. The effect of thrombin inhibitors on dilution-induced hypercoagulation was also examined. It was shown that coagulation was enhanced in plasma diluted up to 2.0–2.5-fold with any PE. This enhancement was due to the dilution of coagulation inhibitors in plasma. Their addition to plasma or PE could partially prevent the hypercoagulation shift. Nature Publishing Group UK 2017-04-12 /pmc/articles/PMC5429808/ /pubmed/28405015 http://dx.doi.org/10.1038/s41598-017-00927-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sinauridze, Elena I.
Gorbatenko, Alexander S.
Seregina, Elena A.
Lipets, Elena N.
Ataullakhanov, Fazoil I.
Moderate plasma dilution using artificial plasma expanders shifts the haemostatic balance to hypercoagulation
title Moderate plasma dilution using artificial plasma expanders shifts the haemostatic balance to hypercoagulation
title_full Moderate plasma dilution using artificial plasma expanders shifts the haemostatic balance to hypercoagulation
title_fullStr Moderate plasma dilution using artificial plasma expanders shifts the haemostatic balance to hypercoagulation
title_full_unstemmed Moderate plasma dilution using artificial plasma expanders shifts the haemostatic balance to hypercoagulation
title_short Moderate plasma dilution using artificial plasma expanders shifts the haemostatic balance to hypercoagulation
title_sort moderate plasma dilution using artificial plasma expanders shifts the haemostatic balance to hypercoagulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429808/
https://www.ncbi.nlm.nih.gov/pubmed/28405015
http://dx.doi.org/10.1038/s41598-017-00927-w
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