Cargando…

Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking

Establishment of continuous cell lines from human normal and tumor tissues is an extended and useful methodology for molecular characterization of cancer pathophysiology and drug development in research laboratories. The exchange of these cell lines between different labs is a common practice that c...

Descripción completa

Detalles Bibliográficos
Autores principales: Corral-Vázquez, C., Aguilar-Quesada, R., Catalina, P., Lucena-Aguilar, G., Ligero, G., Miranda, B., Carrillo-Ávila, J. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429902/
https://www.ncbi.nlm.nih.gov/pubmed/28255773
http://dx.doi.org/10.1007/s10561-017-9617-6
_version_ 1783236123164672000
author Corral-Vázquez, C.
Aguilar-Quesada, R.
Catalina, P.
Lucena-Aguilar, G.
Ligero, G.
Miranda, B.
Carrillo-Ávila, J. A.
author_facet Corral-Vázquez, C.
Aguilar-Quesada, R.
Catalina, P.
Lucena-Aguilar, G.
Ligero, G.
Miranda, B.
Carrillo-Ávila, J. A.
author_sort Corral-Vázquez, C.
collection PubMed
description Establishment of continuous cell lines from human normal and tumor tissues is an extended and useful methodology for molecular characterization of cancer pathophysiology and drug development in research laboratories. The exchange of these cell lines between different labs is a common practice that can compromise assays reliability due to contamination with microorganism such as mycoplasma or cells from different flasks that compromise experiment reproducibility and reliability. Great proportions of cell lines are contaminated with mycoplasma and/or are replaced by cells derived for a different origin during processing or distribution process. The scientific community has underestimated this problem and thousand of research experiment has been done with cell lines that are incorrectly identified and wrong scientific conclusions have been published. Regular contamination and authentication tests are necessary in order to avoid negative consequences of widespread misidentified and contaminated cell lines. Cell banks generate, store and distribute cell lines for research, being mandatory a consistent and continuous quality program. Methods implementation for guaranteeing both, the absence of mycoplasma and authentication in the supplied cell lines, has been performed in the Andalusian Health System Biobank. Specifically, precise results were obtained using real time PCR detection for mycoplasma and 10 STRs identification by capillary electrophoresis for cell line authentication. Advantages and disadvantages of these protocols are discussed.
format Online
Article
Text
id pubmed-5429902
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-54299022017-05-30 Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking Corral-Vázquez, C. Aguilar-Quesada, R. Catalina, P. Lucena-Aguilar, G. Ligero, G. Miranda, B. Carrillo-Ávila, J. A. Cell Tissue Bank Article Establishment of continuous cell lines from human normal and tumor tissues is an extended and useful methodology for molecular characterization of cancer pathophysiology and drug development in research laboratories. The exchange of these cell lines between different labs is a common practice that can compromise assays reliability due to contamination with microorganism such as mycoplasma or cells from different flasks that compromise experiment reproducibility and reliability. Great proportions of cell lines are contaminated with mycoplasma and/or are replaced by cells derived for a different origin during processing or distribution process. The scientific community has underestimated this problem and thousand of research experiment has been done with cell lines that are incorrectly identified and wrong scientific conclusions have been published. Regular contamination and authentication tests are necessary in order to avoid negative consequences of widespread misidentified and contaminated cell lines. Cell banks generate, store and distribute cell lines for research, being mandatory a consistent and continuous quality program. Methods implementation for guaranteeing both, the absence of mycoplasma and authentication in the supplied cell lines, has been performed in the Andalusian Health System Biobank. Specifically, precise results were obtained using real time PCR detection for mycoplasma and 10 STRs identification by capillary electrophoresis for cell line authentication. Advantages and disadvantages of these protocols are discussed. Springer Netherlands 2017-03-02 2017 /pmc/articles/PMC5429902/ /pubmed/28255773 http://dx.doi.org/10.1007/s10561-017-9617-6 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Corral-Vázquez, C.
Aguilar-Quesada, R.
Catalina, P.
Lucena-Aguilar, G.
Ligero, G.
Miranda, B.
Carrillo-Ávila, J. A.
Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking
title Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking
title_full Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking
title_fullStr Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking
title_full_unstemmed Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking
title_short Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking
title_sort cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5429902/
https://www.ncbi.nlm.nih.gov/pubmed/28255773
http://dx.doi.org/10.1007/s10561-017-9617-6
work_keys_str_mv AT corralvazquezc celllinesauthenticationandmycoplasmadetectionasminimunqualitycontrolofcelllinesinbiobanking
AT aguilarquesadar celllinesauthenticationandmycoplasmadetectionasminimunqualitycontrolofcelllinesinbiobanking
AT catalinap celllinesauthenticationandmycoplasmadetectionasminimunqualitycontrolofcelllinesinbiobanking
AT lucenaaguilarg celllinesauthenticationandmycoplasmadetectionasminimunqualitycontrolofcelllinesinbiobanking
AT ligerog celllinesauthenticationandmycoplasmadetectionasminimunqualitycontrolofcelllinesinbiobanking
AT mirandab celllinesauthenticationandmycoplasmadetectionasminimunqualitycontrolofcelllinesinbiobanking
AT carrilloavilaja celllinesauthenticationandmycoplasmadetectionasminimunqualitycontrolofcelllinesinbiobanking