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Immunolocalization of chromosome-associated proteins in plants – principles and applications
The use of the immunolocalization technique combined with cytogenetic and epigenetic studies is an indispensable tool and has contributed significantly to the analysis of the structure and function of chromosomes, since it can provide information about the spatial or temporal distribution of a given...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430351/ https://www.ncbi.nlm.nih.gov/pubmed/28510982 http://dx.doi.org/10.1186/s40529-014-0063-5 |
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author | de Paula, Cristina Maria Pinto Techio, Vânia Helena |
author_facet | de Paula, Cristina Maria Pinto Techio, Vânia Helena |
author_sort | de Paula, Cristina Maria Pinto |
collection | PubMed |
description | The use of the immunolocalization technique combined with cytogenetic and epigenetic studies is an indispensable tool and has contributed significantly to the analysis of the structure and function of chromosomes, since it can provide information about the spatial or temporal distribution of a given protein in the nucleus and chromosomes. Several chromosome-associated proteins in plant cells have already been identified by immunolocalization, such as histone and non-histone proteins and cell division-related protein (mitosis and meiosis). The principle of the immunolocalization technique in plants basically involves fixation and permeabilization of cells, the use of monoclonal or polyclonal antibodies attached to a signaling molecule, usually a fluorochrome and detection of the target molecule by using an epifluorescence microscope. |
format | Online Article Text |
id | pubmed-5430351 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-54303512017-05-30 Immunolocalization of chromosome-associated proteins in plants – principles and applications de Paula, Cristina Maria Pinto Techio, Vânia Helena Bot Stud Review The use of the immunolocalization technique combined with cytogenetic and epigenetic studies is an indispensable tool and has contributed significantly to the analysis of the structure and function of chromosomes, since it can provide information about the spatial or temporal distribution of a given protein in the nucleus and chromosomes. Several chromosome-associated proteins in plant cells have already been identified by immunolocalization, such as histone and non-histone proteins and cell division-related protein (mitosis and meiosis). The principle of the immunolocalization technique in plants basically involves fixation and permeabilization of cells, the use of monoclonal or polyclonal antibodies attached to a signaling molecule, usually a fluorochrome and detection of the target molecule by using an epifluorescence microscope. Springer Berlin Heidelberg 2014-08-05 /pmc/articles/PMC5430351/ /pubmed/28510982 http://dx.doi.org/10.1186/s40529-014-0063-5 Text en © Paula and Techio; licensee Springer 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
spellingShingle | Review de Paula, Cristina Maria Pinto Techio, Vânia Helena Immunolocalization of chromosome-associated proteins in plants – principles and applications |
title | Immunolocalization of chromosome-associated proteins in plants – principles and applications |
title_full | Immunolocalization of chromosome-associated proteins in plants – principles and applications |
title_fullStr | Immunolocalization of chromosome-associated proteins in plants – principles and applications |
title_full_unstemmed | Immunolocalization of chromosome-associated proteins in plants – principles and applications |
title_short | Immunolocalization of chromosome-associated proteins in plants – principles and applications |
title_sort | immunolocalization of chromosome-associated proteins in plants – principles and applications |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430351/ https://www.ncbi.nlm.nih.gov/pubmed/28510982 http://dx.doi.org/10.1186/s40529-014-0063-5 |
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