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In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia
The ability to genetically manipulate trigeminal ganglion (TG) neurons would be useful in the study of the craniofacial nervous system and latent alphaherpesvirus infections. We investigated adeno-associated virus (AAV) vectors for gene delivery to the TG after intradermal whiskerpad delivery in mic...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430444/ https://www.ncbi.nlm.nih.gov/pubmed/28424485 http://dx.doi.org/10.1038/s41598-017-01004-y |
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author | Dang, Chung H. Aubert, Martine De Silva Feelixge, Harshana S. Diem, Kurt Loprieno, Michelle A. Roychoudhury, Pavitra Stone, Daniel Jerome, Keith R. |
author_facet | Dang, Chung H. Aubert, Martine De Silva Feelixge, Harshana S. Diem, Kurt Loprieno, Michelle A. Roychoudhury, Pavitra Stone, Daniel Jerome, Keith R. |
author_sort | Dang, Chung H. |
collection | PubMed |
description | The ability to genetically manipulate trigeminal ganglion (TG) neurons would be useful in the study of the craniofacial nervous system and latent alphaherpesvirus infections. We investigated adeno-associated virus (AAV) vectors for gene delivery to the TG after intradermal whiskerpad delivery in mice. We demonstrated that AAV vectors of serotypes 1, 7, 8, and 9 trafficked from the whiskerpad into TG neurons and expressed transgenes within cell bodies and axons of sensory neurons in all three branches of the TG. Gene expression was highest with AAV1, and steadily increased over time up to day 28. Both constitutive and neuronal-specific promoters were able to drive transgene expression in TG neurons. Levels of vector genomes in the TG increased with input dose, and multiple transgenes could be co-delivered to TG neurons by separate AAV vectors. In conclusion, AAV1 vectors are suitable for gene delivery to TG sensory neurons following intradermal whiskerpad injection. |
format | Online Article Text |
id | pubmed-5430444 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54304442017-05-15 In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia Dang, Chung H. Aubert, Martine De Silva Feelixge, Harshana S. Diem, Kurt Loprieno, Michelle A. Roychoudhury, Pavitra Stone, Daniel Jerome, Keith R. Sci Rep Article The ability to genetically manipulate trigeminal ganglion (TG) neurons would be useful in the study of the craniofacial nervous system and latent alphaherpesvirus infections. We investigated adeno-associated virus (AAV) vectors for gene delivery to the TG after intradermal whiskerpad delivery in mice. We demonstrated that AAV vectors of serotypes 1, 7, 8, and 9 trafficked from the whiskerpad into TG neurons and expressed transgenes within cell bodies and axons of sensory neurons in all three branches of the TG. Gene expression was highest with AAV1, and steadily increased over time up to day 28. Both constitutive and neuronal-specific promoters were able to drive transgene expression in TG neurons. Levels of vector genomes in the TG increased with input dose, and multiple transgenes could be co-delivered to TG neurons by separate AAV vectors. In conclusion, AAV1 vectors are suitable for gene delivery to TG sensory neurons following intradermal whiskerpad injection. Nature Publishing Group UK 2017-04-19 /pmc/articles/PMC5430444/ /pubmed/28424485 http://dx.doi.org/10.1038/s41598-017-01004-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Dang, Chung H. Aubert, Martine De Silva Feelixge, Harshana S. Diem, Kurt Loprieno, Michelle A. Roychoudhury, Pavitra Stone, Daniel Jerome, Keith R. In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia |
title | In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia |
title_full | In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia |
title_fullStr | In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia |
title_full_unstemmed | In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia |
title_short | In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the trigeminal ganglia |
title_sort | in vivo dynamics of aav-mediated gene delivery to sensory neurons of the trigeminal ganglia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430444/ https://www.ncbi.nlm.nih.gov/pubmed/28424485 http://dx.doi.org/10.1038/s41598-017-01004-y |
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