Cargando…

The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei

Profiling of extracts from twelve propolis samples collected from eight regions in Nigeria was carried out using high performance liquid chromatography (LC) coupled with evaporative light scattering (ELSD), ultraviolet detection (UV) and mass spectrometry (MS), gas chromatography mass spectrometry (...

Descripción completa

Detalles Bibliográficos
Autores principales: Omar, Ruwida, Igoli, John O., Zhang, Tong, Gray, Alexander I., Ebiloma, Godwin U., Clements, Carol J., Fearnley, James, Edrada Ebel, RuAngeli, Paget, Tim, de Koning, Harry P., Watson, David G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430459/
https://www.ncbi.nlm.nih.gov/pubmed/28424496
http://dx.doi.org/10.1038/s41598-017-01038-2
_version_ 1783236219975499776
author Omar, Ruwida
Igoli, John O.
Zhang, Tong
Gray, Alexander I.
Ebiloma, Godwin U.
Clements, Carol J.
Fearnley, James
Edrada Ebel, RuAngeli
Paget, Tim
de Koning, Harry P.
Watson, David G.
author_facet Omar, Ruwida
Igoli, John O.
Zhang, Tong
Gray, Alexander I.
Ebiloma, Godwin U.
Clements, Carol J.
Fearnley, James
Edrada Ebel, RuAngeli
Paget, Tim
de Koning, Harry P.
Watson, David G.
author_sort Omar, Ruwida
collection PubMed
description Profiling of extracts from twelve propolis samples collected from eight regions in Nigeria was carried out using high performance liquid chromatography (LC) coupled with evaporative light scattering (ELSD), ultraviolet detection (UV) and mass spectrometry (MS), gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). Principal component analysis (PCA) of the processed LC-MS data demonstrated the varying chemical composition of the samples. Most of the samples were active against Trypanosoma b. brucei with the highest activity being in the samples from Southern Nigeria. The more active samples were fractionated in order to isolate the component(s) responsible for their activity using medium pressure liquid chromatography (MPLC). Three xanthones, 1,3,7-trihydroxy-2,8-di-(3-methylbut-2-enyl)xanthone, 1,3,7-trihydroxy-4,8-di-(3-methylbut-2-enyl)xanthone a previously undescribed xanthone and three triterpenes: ambonic acid, mangiferonic acid and a mixture of α-amyrin with mangiferonic acid (1:3) were isolated and characterised by NMR and LC-MS. These compounds all displayed strong inhibitory activity against T.b. brucei but none of them had higher activity than the crude extracts. Partial least squares (PLS) modelling of the anti-trypanosomal activity of the sample extracts using the LC-MS data indicated that high activity in the extracts, as judged from LCMS(2) data, could be correlated to denticulatain isomers in the extracts.
format Online
Article
Text
id pubmed-5430459
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-54304592017-05-15 The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei Omar, Ruwida Igoli, John O. Zhang, Tong Gray, Alexander I. Ebiloma, Godwin U. Clements, Carol J. Fearnley, James Edrada Ebel, RuAngeli Paget, Tim de Koning, Harry P. Watson, David G. Sci Rep Article Profiling of extracts from twelve propolis samples collected from eight regions in Nigeria was carried out using high performance liquid chromatography (LC) coupled with evaporative light scattering (ELSD), ultraviolet detection (UV) and mass spectrometry (MS), gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). Principal component analysis (PCA) of the processed LC-MS data demonstrated the varying chemical composition of the samples. Most of the samples were active against Trypanosoma b. brucei with the highest activity being in the samples from Southern Nigeria. The more active samples were fractionated in order to isolate the component(s) responsible for their activity using medium pressure liquid chromatography (MPLC). Three xanthones, 1,3,7-trihydroxy-2,8-di-(3-methylbut-2-enyl)xanthone, 1,3,7-trihydroxy-4,8-di-(3-methylbut-2-enyl)xanthone a previously undescribed xanthone and three triterpenes: ambonic acid, mangiferonic acid and a mixture of α-amyrin with mangiferonic acid (1:3) were isolated and characterised by NMR and LC-MS. These compounds all displayed strong inhibitory activity against T.b. brucei but none of them had higher activity than the crude extracts. Partial least squares (PLS) modelling of the anti-trypanosomal activity of the sample extracts using the LC-MS data indicated that high activity in the extracts, as judged from LCMS(2) data, could be correlated to denticulatain isomers in the extracts. Nature Publishing Group UK 2017-04-19 /pmc/articles/PMC5430459/ /pubmed/28424496 http://dx.doi.org/10.1038/s41598-017-01038-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Omar, Ruwida
Igoli, John O.
Zhang, Tong
Gray, Alexander I.
Ebiloma, Godwin U.
Clements, Carol J.
Fearnley, James
Edrada Ebel, RuAngeli
Paget, Tim
de Koning, Harry P.
Watson, David G.
The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei
title The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei
title_full The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei
title_fullStr The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei
title_full_unstemmed The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei
title_short The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei
title_sort chemical characterization of nigerian propolis samples and their activity against trypanosoma brucei
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430459/
https://www.ncbi.nlm.nih.gov/pubmed/28424496
http://dx.doi.org/10.1038/s41598-017-01038-2
work_keys_str_mv AT omarruwida thechemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT igolijohno thechemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT zhangtong thechemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT grayalexanderi thechemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT ebilomagodwinu thechemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT clementscarolj thechemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT fearnleyjames thechemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT edradaebelruangeli thechemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT pagettim thechemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT dekoningharryp thechemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT watsondavidg thechemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT omarruwida chemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT igolijohno chemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT zhangtong chemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT grayalexanderi chemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT ebilomagodwinu chemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT clementscarolj chemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT fearnleyjames chemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT edradaebelruangeli chemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT pagettim chemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT dekoningharryp chemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei
AT watsondavidg chemicalcharacterizationofnigerianpropolissamplesandtheiractivityagainsttrypanosomabrucei