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The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei
Profiling of extracts from twelve propolis samples collected from eight regions in Nigeria was carried out using high performance liquid chromatography (LC) coupled with evaporative light scattering (ELSD), ultraviolet detection (UV) and mass spectrometry (MS), gas chromatography mass spectrometry (...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430459/ https://www.ncbi.nlm.nih.gov/pubmed/28424496 http://dx.doi.org/10.1038/s41598-017-01038-2 |
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author | Omar, Ruwida Igoli, John O. Zhang, Tong Gray, Alexander I. Ebiloma, Godwin U. Clements, Carol J. Fearnley, James Edrada Ebel, RuAngeli Paget, Tim de Koning, Harry P. Watson, David G. |
author_facet | Omar, Ruwida Igoli, John O. Zhang, Tong Gray, Alexander I. Ebiloma, Godwin U. Clements, Carol J. Fearnley, James Edrada Ebel, RuAngeli Paget, Tim de Koning, Harry P. Watson, David G. |
author_sort | Omar, Ruwida |
collection | PubMed |
description | Profiling of extracts from twelve propolis samples collected from eight regions in Nigeria was carried out using high performance liquid chromatography (LC) coupled with evaporative light scattering (ELSD), ultraviolet detection (UV) and mass spectrometry (MS), gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). Principal component analysis (PCA) of the processed LC-MS data demonstrated the varying chemical composition of the samples. Most of the samples were active against Trypanosoma b. brucei with the highest activity being in the samples from Southern Nigeria. The more active samples were fractionated in order to isolate the component(s) responsible for their activity using medium pressure liquid chromatography (MPLC). Three xanthones, 1,3,7-trihydroxy-2,8-di-(3-methylbut-2-enyl)xanthone, 1,3,7-trihydroxy-4,8-di-(3-methylbut-2-enyl)xanthone a previously undescribed xanthone and three triterpenes: ambonic acid, mangiferonic acid and a mixture of α-amyrin with mangiferonic acid (1:3) were isolated and characterised by NMR and LC-MS. These compounds all displayed strong inhibitory activity against T.b. brucei but none of them had higher activity than the crude extracts. Partial least squares (PLS) modelling of the anti-trypanosomal activity of the sample extracts using the LC-MS data indicated that high activity in the extracts, as judged from LCMS(2) data, could be correlated to denticulatain isomers in the extracts. |
format | Online Article Text |
id | pubmed-5430459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54304592017-05-15 The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei Omar, Ruwida Igoli, John O. Zhang, Tong Gray, Alexander I. Ebiloma, Godwin U. Clements, Carol J. Fearnley, James Edrada Ebel, RuAngeli Paget, Tim de Koning, Harry P. Watson, David G. Sci Rep Article Profiling of extracts from twelve propolis samples collected from eight regions in Nigeria was carried out using high performance liquid chromatography (LC) coupled with evaporative light scattering (ELSD), ultraviolet detection (UV) and mass spectrometry (MS), gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). Principal component analysis (PCA) of the processed LC-MS data demonstrated the varying chemical composition of the samples. Most of the samples were active against Trypanosoma b. brucei with the highest activity being in the samples from Southern Nigeria. The more active samples were fractionated in order to isolate the component(s) responsible for their activity using medium pressure liquid chromatography (MPLC). Three xanthones, 1,3,7-trihydroxy-2,8-di-(3-methylbut-2-enyl)xanthone, 1,3,7-trihydroxy-4,8-di-(3-methylbut-2-enyl)xanthone a previously undescribed xanthone and three triterpenes: ambonic acid, mangiferonic acid and a mixture of α-amyrin with mangiferonic acid (1:3) were isolated and characterised by NMR and LC-MS. These compounds all displayed strong inhibitory activity against T.b. brucei but none of them had higher activity than the crude extracts. Partial least squares (PLS) modelling of the anti-trypanosomal activity of the sample extracts using the LC-MS data indicated that high activity in the extracts, as judged from LCMS(2) data, could be correlated to denticulatain isomers in the extracts. Nature Publishing Group UK 2017-04-19 /pmc/articles/PMC5430459/ /pubmed/28424496 http://dx.doi.org/10.1038/s41598-017-01038-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Omar, Ruwida Igoli, John O. Zhang, Tong Gray, Alexander I. Ebiloma, Godwin U. Clements, Carol J. Fearnley, James Edrada Ebel, RuAngeli Paget, Tim de Koning, Harry P. Watson, David G. The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei |
title | The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei |
title_full | The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei |
title_fullStr | The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei |
title_full_unstemmed | The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei |
title_short | The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei |
title_sort | chemical characterization of nigerian propolis samples and their activity against trypanosoma brucei |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430459/ https://www.ncbi.nlm.nih.gov/pubmed/28424496 http://dx.doi.org/10.1038/s41598-017-01038-2 |
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