Pulmonary delivery of triptolide-loaded liposomes decorated with anti-carbonic anhydrase IX antibody for lung cancer therapy

Antibody-decorated liposomes can facilitate the precise delivery of chemotherapeutic drugs to the lung by targeting a recognition factor present on the surface of lung tumor cells. Carbonic anhydrase IX (CA IX) is an enzyme expressed on the surface of lung cancer cells with a restricted expression i...

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Autores principales: Lin, Congcong, Wong, Blenda Chi Kwan, Chen, Hubiao, Bian, Zhaoxiang, Zhang, Ge, Zhang, Xue, Kashif Riaz, Muhammad, Tyagi, Deependra, Lin, Ge, Zhang, Yanbo, Wang, Jinjin, Lu, Aiping, Yang, Zhijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430522/
https://www.ncbi.nlm.nih.gov/pubmed/28428618
http://dx.doi.org/10.1038/s41598-017-00957-4
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author Lin, Congcong
Wong, Blenda Chi Kwan
Chen, Hubiao
Bian, Zhaoxiang
Zhang, Ge
Zhang, Xue
Kashif Riaz, Muhammad
Tyagi, Deependra
Lin, Ge
Zhang, Yanbo
Wang, Jinjin
Lu, Aiping
Yang, Zhijun
author_facet Lin, Congcong
Wong, Blenda Chi Kwan
Chen, Hubiao
Bian, Zhaoxiang
Zhang, Ge
Zhang, Xue
Kashif Riaz, Muhammad
Tyagi, Deependra
Lin, Ge
Zhang, Yanbo
Wang, Jinjin
Lu, Aiping
Yang, Zhijun
author_sort Lin, Congcong
collection PubMed
description Antibody-decorated liposomes can facilitate the precise delivery of chemotherapeutic drugs to the lung by targeting a recognition factor present on the surface of lung tumor cells. Carbonic anhydrase IX (CA IX) is an enzyme expressed on the surface of lung cancer cells with a restricted expression in normal lungs. Here, we explored the utility of anti-carbonic anhydrase IX (CA IX) antibody, conjugated to the surface of triptolide (TPL)-loaded liposomes (CA IX-TPL-Lips), to promote the therapeutic effects for lung cancer via pulmonary administration. It was found that the CA IX-TPL-Lips significantly improved the cellular uptake efficiency in both CA IX-positive human non-small cell lung cancer cells (A549) and A549 tumor spheroids, resulting in the efficient cell killing compared with free TPL and non-targeted TPL-Lips. In vivo, CA IX-Lips via pulmonary delivery showed specificity and a sustained release property resided up to 96 h in the lung, both of which improved the efficiency of TPL formulations in restraining tumor growth and significantly prolonged the lifespan of mice with orthotopic lung tumors. The results suggest that CA IX-decorated liposomes can potentially be used as an effective therapeutic strategy for lung cancer.
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spelling pubmed-54305222017-05-15 Pulmonary delivery of triptolide-loaded liposomes decorated with anti-carbonic anhydrase IX antibody for lung cancer therapy Lin, Congcong Wong, Blenda Chi Kwan Chen, Hubiao Bian, Zhaoxiang Zhang, Ge Zhang, Xue Kashif Riaz, Muhammad Tyagi, Deependra Lin, Ge Zhang, Yanbo Wang, Jinjin Lu, Aiping Yang, Zhijun Sci Rep Article Antibody-decorated liposomes can facilitate the precise delivery of chemotherapeutic drugs to the lung by targeting a recognition factor present on the surface of lung tumor cells. Carbonic anhydrase IX (CA IX) is an enzyme expressed on the surface of lung cancer cells with a restricted expression in normal lungs. Here, we explored the utility of anti-carbonic anhydrase IX (CA IX) antibody, conjugated to the surface of triptolide (TPL)-loaded liposomes (CA IX-TPL-Lips), to promote the therapeutic effects for lung cancer via pulmonary administration. It was found that the CA IX-TPL-Lips significantly improved the cellular uptake efficiency in both CA IX-positive human non-small cell lung cancer cells (A549) and A549 tumor spheroids, resulting in the efficient cell killing compared with free TPL and non-targeted TPL-Lips. In vivo, CA IX-Lips via pulmonary delivery showed specificity and a sustained release property resided up to 96 h in the lung, both of which improved the efficiency of TPL formulations in restraining tumor growth and significantly prolonged the lifespan of mice with orthotopic lung tumors. The results suggest that CA IX-decorated liposomes can potentially be used as an effective therapeutic strategy for lung cancer. Nature Publishing Group UK 2017-04-20 /pmc/articles/PMC5430522/ /pubmed/28428618 http://dx.doi.org/10.1038/s41598-017-00957-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lin, Congcong
Wong, Blenda Chi Kwan
Chen, Hubiao
Bian, Zhaoxiang
Zhang, Ge
Zhang, Xue
Kashif Riaz, Muhammad
Tyagi, Deependra
Lin, Ge
Zhang, Yanbo
Wang, Jinjin
Lu, Aiping
Yang, Zhijun
Pulmonary delivery of triptolide-loaded liposomes decorated with anti-carbonic anhydrase IX antibody for lung cancer therapy
title Pulmonary delivery of triptolide-loaded liposomes decorated with anti-carbonic anhydrase IX antibody for lung cancer therapy
title_full Pulmonary delivery of triptolide-loaded liposomes decorated with anti-carbonic anhydrase IX antibody for lung cancer therapy
title_fullStr Pulmonary delivery of triptolide-loaded liposomes decorated with anti-carbonic anhydrase IX antibody for lung cancer therapy
title_full_unstemmed Pulmonary delivery of triptolide-loaded liposomes decorated with anti-carbonic anhydrase IX antibody for lung cancer therapy
title_short Pulmonary delivery of triptolide-loaded liposomes decorated with anti-carbonic anhydrase IX antibody for lung cancer therapy
title_sort pulmonary delivery of triptolide-loaded liposomes decorated with anti-carbonic anhydrase ix antibody for lung cancer therapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430522/
https://www.ncbi.nlm.nih.gov/pubmed/28428618
http://dx.doi.org/10.1038/s41598-017-00957-4
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