Effects of volatile organic compound ether on cell responses and gene expressions in Arabidopsis

BACKGROUND: The volatile organic compound ether is widely used as an industrial solvent and easily released to the environment. Our previous research indicated that ether triggers reactive oxygen species (ROS) production and activates ethylene biosynthetic genes and defense gene expressions in tomato. In the present study, we investigated the effect of ether on cell responses and gene expressions in Arabidopsis and compared the ROS and phytohormones produced in Arabidopsis and tomato plants in response to different air pollutants (O(3) vs. ether). RESULTS: Ether induced the sequential production of superoxide anion and hydrogen peroxide in Arabidopsis. Ether also triggered expressions of ethylene, salicylic acid and jasmonic acid biosynthetic genes. The temporal expression patterns of MAP kinase and protein phosphatase genes are in good accordance with those of the ethylene and salicylic acid biosynthetic genes, suggesting that induction of these phytohormone biosynthesis were through signaling pathways including both phosphorylation and/or dephosphorylation. By contrast, expression pattern of protein phosphatase PP2A3&4 coincided well with the expression of jasmonic acid biosynthetic gene LOX4, suggesting that induction of jasmonic acid biosynthesis is through PP2A3&4. However, the production of ROS and temporal expression patterns of phytohormone biosynthetic genes in Arabidopsis in response to ether were different from those to O(3) and were different from those in tomato as well. CONCLUSIONS: Different plants have different strategies to respond to the same abiotic stress, and each plant species possesses its own unique signaling pathways that regulate the responding process. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40529-015-0112-8) contains supplementary material, which is available to authorized users.