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Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas

BACKGROUND: The unicellular green alga, Chlamydomonas reinhardtii, is a classic model for studying flagella and biofuel. However, precise gene editing, such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas9) system, is not widely used in this o...

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Detalles Bibliográficos
Autores principales:	Cheng, Xi, Liu, Gai, Ke, Wenting, Zhao, Lijuan, Lv, Bo, Ma, Xiaocui, Xu, Nannan, Xia, Xiaoling, Deng, Xuan, Zheng, Chunlei, Huang, Kaiyao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430608/ https://www.ncbi.nlm.nih.gov/pubmed/28515773 http://dx.doi.org/10.1186/s13007-017-0183-5

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