Regulation of viral gene expression by duck enteritis virus UL54

Duck enteritis virus (DEV) UL54 is a homologue of human herpes simplex virus-1 (HSV-1) ICP27, which plays essential regulatory roles during infection. Our previous studies indicated that DEV UL54 is an immediate-early protein that can shuttle between the nucleus and the cytoplasm. In the present stu...

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Autores principales: Liu, Chaoyue, Cheng, Anchun, Wang, Mingshu, Chen, Shun, Jia, Renyong, Zhu, Dekang, Liu, Mafeng, Sun, Kunfeng, Yang, Qiao, Wu, Ying, Zhao, Xinxin, Chen, Xiaoyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430722/
https://www.ncbi.nlm.nih.gov/pubmed/28432334
http://dx.doi.org/10.1038/s41598-017-01161-0
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author Liu, Chaoyue
Cheng, Anchun
Wang, Mingshu
Chen, Shun
Jia, Renyong
Zhu, Dekang
Liu, Mafeng
Sun, Kunfeng
Yang, Qiao
Wu, Ying
Zhao, Xinxin
Chen, Xiaoyue
author_facet Liu, Chaoyue
Cheng, Anchun
Wang, Mingshu
Chen, Shun
Jia, Renyong
Zhu, Dekang
Liu, Mafeng
Sun, Kunfeng
Yang, Qiao
Wu, Ying
Zhao, Xinxin
Chen, Xiaoyue
author_sort Liu, Chaoyue
collection PubMed
description Duck enteritis virus (DEV) UL54 is a homologue of human herpes simplex virus-1 (HSV-1) ICP27, which plays essential regulatory roles during infection. Our previous studies indicated that DEV UL54 is an immediate-early protein that can shuttle between the nucleus and the cytoplasm. In the present study, we found that UL54-deleted DEV (DEV-ΔUL54) exhibits growth kinetics, a plaque size and a viral DNA copy number that are significantly different from those of its parent wild-type virus (DEV-LoxP) and the revertant (DEV-ΔUL54 (Revertant)). Relative viral mRNA levels, reflecting gene expression, the transcription phase and the translation stage, are also significantly different between DEV-ΔUL54-infected cells and DEV-LoxP/DEV-ΔUL54 (Revertant)-infected cells. However, the localization pattern of UL30 mRNA is obviously changed in DEV-ΔUL54-infected cells. These findings suggest that DEV UL54 is important for virus growth and may regulate viral gene expression during transcription, mRNA export and translation.
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spelling pubmed-54307222017-05-16 Regulation of viral gene expression by duck enteritis virus UL54 Liu, Chaoyue Cheng, Anchun Wang, Mingshu Chen, Shun Jia, Renyong Zhu, Dekang Liu, Mafeng Sun, Kunfeng Yang, Qiao Wu, Ying Zhao, Xinxin Chen, Xiaoyue Sci Rep Article Duck enteritis virus (DEV) UL54 is a homologue of human herpes simplex virus-1 (HSV-1) ICP27, which plays essential regulatory roles during infection. Our previous studies indicated that DEV UL54 is an immediate-early protein that can shuttle between the nucleus and the cytoplasm. In the present study, we found that UL54-deleted DEV (DEV-ΔUL54) exhibits growth kinetics, a plaque size and a viral DNA copy number that are significantly different from those of its parent wild-type virus (DEV-LoxP) and the revertant (DEV-ΔUL54 (Revertant)). Relative viral mRNA levels, reflecting gene expression, the transcription phase and the translation stage, are also significantly different between DEV-ΔUL54-infected cells and DEV-LoxP/DEV-ΔUL54 (Revertant)-infected cells. However, the localization pattern of UL30 mRNA is obviously changed in DEV-ΔUL54-infected cells. These findings suggest that DEV UL54 is important for virus growth and may regulate viral gene expression during transcription, mRNA export and translation. Nature Publishing Group UK 2017-04-21 /pmc/articles/PMC5430722/ /pubmed/28432334 http://dx.doi.org/10.1038/s41598-017-01161-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Liu, Chaoyue
Cheng, Anchun
Wang, Mingshu
Chen, Shun
Jia, Renyong
Zhu, Dekang
Liu, Mafeng
Sun, Kunfeng
Yang, Qiao
Wu, Ying
Zhao, Xinxin
Chen, Xiaoyue
Regulation of viral gene expression by duck enteritis virus UL54
title Regulation of viral gene expression by duck enteritis virus UL54
title_full Regulation of viral gene expression by duck enteritis virus UL54
title_fullStr Regulation of viral gene expression by duck enteritis virus UL54
title_full_unstemmed Regulation of viral gene expression by duck enteritis virus UL54
title_short Regulation of viral gene expression by duck enteritis virus UL54
title_sort regulation of viral gene expression by duck enteritis virus ul54
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430722/
https://www.ncbi.nlm.nih.gov/pubmed/28432334
http://dx.doi.org/10.1038/s41598-017-01161-0
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