Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation

Taxol, a first-line anti-tumour drug, has low effectiveness against colorectal cancer. Combination with other agents is an effective strategy to enhance Taxol cytotoxicity. Kanglaite injection is an extract from Coix lacryma-jobi seed and is usually combined with other agents to treat cancer. The ai...

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Autores principales: Wang, Yijia, Zhang, Chunze, Zhang, Shiwu, Zhao, Zhenying, Wang, Jiawen, Song, Jiali, Wang, Yue, Liu, Jun, Hou, Shaobin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430786/
https://www.ncbi.nlm.nih.gov/pubmed/28455529
http://dx.doi.org/10.1038/s41598-017-01480-2
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author Wang, Yijia
Zhang, Chunze
Zhang, Shiwu
Zhao, Zhenying
Wang, Jiawen
Song, Jiali
Wang, Yue
Liu, Jun
Hou, Shaobin
author_facet Wang, Yijia
Zhang, Chunze
Zhang, Shiwu
Zhao, Zhenying
Wang, Jiawen
Song, Jiali
Wang, Yue
Liu, Jun
Hou, Shaobin
author_sort Wang, Yijia
collection PubMed
description Taxol, a first-line anti-tumour drug, has low effectiveness against colorectal cancer. Combination with other agents is an effective strategy to enhance Taxol cytotoxicity. Kanglaite injection is an extract from Coix lacryma-jobi seed and is usually combined with other agents to treat cancer. The aim of this study was to investigate the treatment effect of Taxol combined with Kanglaite on colorectal cancer cell lines. Kanglaite pretreatment followed by Taxol treatment was found to show the best synergism among all combination strategies. This combination also resulted in the smallest tumour volume in a Balb/c mice model. Kanglaite inhibited the expression of nuclear factor (NF)-κΒ and upregulated that of connexin 43, both of which sensitized cancer cells to Taxol. Moreover, Kanglaite increased many cellular variations caused by Taxol, including tubulin polymerization, caspase-3 cleavage, and upregulated expression of survivin and cyclin B1. These results suggest that Kanglaite pretreatment may increase the effect of Taxol on colorectal cancer.
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spelling pubmed-54307862017-05-16 Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation Wang, Yijia Zhang, Chunze Zhang, Shiwu Zhao, Zhenying Wang, Jiawen Song, Jiali Wang, Yue Liu, Jun Hou, Shaobin Sci Rep Article Taxol, a first-line anti-tumour drug, has low effectiveness against colorectal cancer. Combination with other agents is an effective strategy to enhance Taxol cytotoxicity. Kanglaite injection is an extract from Coix lacryma-jobi seed and is usually combined with other agents to treat cancer. The aim of this study was to investigate the treatment effect of Taxol combined with Kanglaite on colorectal cancer cell lines. Kanglaite pretreatment followed by Taxol treatment was found to show the best synergism among all combination strategies. This combination also resulted in the smallest tumour volume in a Balb/c mice model. Kanglaite inhibited the expression of nuclear factor (NF)-κΒ and upregulated that of connexin 43, both of which sensitized cancer cells to Taxol. Moreover, Kanglaite increased many cellular variations caused by Taxol, including tubulin polymerization, caspase-3 cleavage, and upregulated expression of survivin and cyclin B1. These results suggest that Kanglaite pretreatment may increase the effect of Taxol on colorectal cancer. Nature Publishing Group UK 2017-04-28 /pmc/articles/PMC5430786/ /pubmed/28455529 http://dx.doi.org/10.1038/s41598-017-01480-2 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Yijia
Zhang, Chunze
Zhang, Shiwu
Zhao, Zhenying
Wang, Jiawen
Song, Jiali
Wang, Yue
Liu, Jun
Hou, Shaobin
Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation
title Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation
title_full Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation
title_fullStr Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation
title_full_unstemmed Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation
title_short Kanglaite sensitizes colorectal cancer cells to Taxol via NF-κΒ inhibition and connexin 43 upregulation
title_sort kanglaite sensitizes colorectal cancer cells to taxol via nf-κβ inhibition and connexin 43 upregulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430786/
https://www.ncbi.nlm.nih.gov/pubmed/28455529
http://dx.doi.org/10.1038/s41598-017-01480-2
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