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Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet

The variation in the biological function of proteins plays an important role in plasma medicine and sterilization. Several non-thermal plasma sources with different feeding gases are used worldwide for plasma treatment, including dielectric barrier discharge (DBD) and atmospheric-pressure plasma jet...

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Autores principales: Choi, Sooho, Attri, Pankaj, Lee, Inhwan, Oh, Jeongmin, Yun, Ji-Hye, Park, Ji Hoon, Choi, Eun Ha, Lee, Weontae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430822/
https://www.ncbi.nlm.nih.gov/pubmed/28432354
http://dx.doi.org/10.1038/s41598-017-01030-w
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author Choi, Sooho
Attri, Pankaj
Lee, Inhwan
Oh, Jeongmin
Yun, Ji-Hye
Park, Ji Hoon
Choi, Eun Ha
Lee, Weontae
author_facet Choi, Sooho
Attri, Pankaj
Lee, Inhwan
Oh, Jeongmin
Yun, Ji-Hye
Park, Ji Hoon
Choi, Eun Ha
Lee, Weontae
author_sort Choi, Sooho
collection PubMed
description The variation in the biological function of proteins plays an important role in plasma medicine and sterilization. Several non-thermal plasma sources with different feeding gases are used worldwide for plasma treatment, including dielectric barrier discharge (DBD) and atmospheric-pressure plasma jet (APPJ) as the most commonly used sources. Therefore, in the present work, we used both DBD and APPJ plasma sources with N(2) and air as feeding gases to evaluate the effects on the structural, thermodynamic, and activity changes of enzymes. In the current work, we used lysozyme as a model enzyme and verified the structural changes using circular dichroism (CD), fluorescence, and X-ray crystallography. In addition, we investigated the lysozyme thermodynamics using CD thermal analysis and changes in the B-factor from X-ray crystallography. The results showed that lysozyme activity decreased after the plasma treatment. From these analyses, we concluded that N(2)-feeding gas plasma disturbs the structure and activity of lysozyme more than Air feeding gas plasma in our experimental studies. This study provides novel fundamental information on the changes to enzymes upon plasma treatment, which has been absent from the literature until now.
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spelling pubmed-54308222017-05-16 Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet Choi, Sooho Attri, Pankaj Lee, Inhwan Oh, Jeongmin Yun, Ji-Hye Park, Ji Hoon Choi, Eun Ha Lee, Weontae Sci Rep Article The variation in the biological function of proteins plays an important role in plasma medicine and sterilization. Several non-thermal plasma sources with different feeding gases are used worldwide for plasma treatment, including dielectric barrier discharge (DBD) and atmospheric-pressure plasma jet (APPJ) as the most commonly used sources. Therefore, in the present work, we used both DBD and APPJ plasma sources with N(2) and air as feeding gases to evaluate the effects on the structural, thermodynamic, and activity changes of enzymes. In the current work, we used lysozyme as a model enzyme and verified the structural changes using circular dichroism (CD), fluorescence, and X-ray crystallography. In addition, we investigated the lysozyme thermodynamics using CD thermal analysis and changes in the B-factor from X-ray crystallography. The results showed that lysozyme activity decreased after the plasma treatment. From these analyses, we concluded that N(2)-feeding gas plasma disturbs the structure and activity of lysozyme more than Air feeding gas plasma in our experimental studies. This study provides novel fundamental information on the changes to enzymes upon plasma treatment, which has been absent from the literature until now. Nature Publishing Group UK 2017-04-21 /pmc/articles/PMC5430822/ /pubmed/28432354 http://dx.doi.org/10.1038/s41598-017-01030-w Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Choi, Sooho
Attri, Pankaj
Lee, Inhwan
Oh, Jeongmin
Yun, Ji-Hye
Park, Ji Hoon
Choi, Eun Ha
Lee, Weontae
Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
title Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
title_full Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
title_fullStr Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
title_full_unstemmed Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
title_short Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
title_sort structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430822/
https://www.ncbi.nlm.nih.gov/pubmed/28432354
http://dx.doi.org/10.1038/s41598-017-01030-w
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