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Genome editing in the mushroom-forming basidiomycete Coprinopsis cinerea, optimized by a high-throughput transformation system

Mushroom-forming basidiomycetes produce a wide range of metabolites and have great value not only as food but also as an important global natural resource. Here, we demonstrate CRISPR/Cas9-based genome editing in the model species Coprinopsis cinerea. Using a high-throughput reporter assay with cryo...

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Detalles Bibliográficos
Autores principales: Sugano, Shigeo S., Suzuki, Hiroko, Shimokita, Eisuke, Chiba, Hirofumi, Noji, Sumihare, Osakabe, Yuriko, Osakabe, Keishi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430836/
https://www.ncbi.nlm.nih.gov/pubmed/28455526
http://dx.doi.org/10.1038/s41598-017-00883-5