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Genome editing in the mushroom-forming basidiomycete Coprinopsis cinerea, optimized by a high-throughput transformation system
Mushroom-forming basidiomycetes produce a wide range of metabolites and have great value not only as food but also as an important global natural resource. Here, we demonstrate CRISPR/Cas9-based genome editing in the model species Coprinopsis cinerea. Using a high-throughput reporter assay with cryo...
Autores principales: | Sugano, Shigeo S., Suzuki, Hiroko, Shimokita, Eisuke, Chiba, Hirofumi, Noji, Sumihare, Osakabe, Yuriko, Osakabe, Keishi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430836/ https://www.ncbi.nlm.nih.gov/pubmed/28455526 http://dx.doi.org/10.1038/s41598-017-00883-5 |
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