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Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay

Differentiation of Brucella canis from other Brucella species are mainly performed through PCR-based methods and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) procedures. Both PCR-based and MLVA methods are limited in discriminating B. canis strains. A new MLVA-13Bc method for B. c...

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Autores principales: Yang, Yi, Wang, Yin, Poulsen, Elizabeth, Ransburgh, Russell, Liu, Xuming, An, Baoyan, Lu, Nanyan, Anderson, Gary, Wang, Chengming, Bai, Jianfa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430899/
https://www.ncbi.nlm.nih.gov/pubmed/28432327
http://dx.doi.org/10.1038/s41598-017-01114-7
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author Yang, Yi
Wang, Yin
Poulsen, Elizabeth
Ransburgh, Russell
Liu, Xuming
An, Baoyan
Lu, Nanyan
Anderson, Gary
Wang, Chengming
Bai, Jianfa
author_facet Yang, Yi
Wang, Yin
Poulsen, Elizabeth
Ransburgh, Russell
Liu, Xuming
An, Baoyan
Lu, Nanyan
Anderson, Gary
Wang, Chengming
Bai, Jianfa
author_sort Yang, Yi
collection PubMed
description Differentiation of Brucella canis from other Brucella species are mainly performed through PCR-based methods and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) procedures. Both PCR-based and MLVA methods are limited in discriminating B. canis strains. A new MLVA-13Bc method for B. canis genotyping was established by combining eight newly-developed VNTRs with five published ones. During 2010 and 2016, 377 B. canis PCR-positives were identified from 6,844 canine blood samples from 22 U.S. states, resulting in 229 B. canis isolates. The MLVA-13Bc method was able to differentiate each of these 229 isolates. The Hunter-Gaston Discriminatory Index of the individual VNTR loci ranged from 0.516 to 0.934 and the combined discriminatory index reached 1.000. Three major clusters (A, B and C) and 10 genotype groups were identified from the 229 B. canis isolates. Cluster A mainly contains genotype groups 1 and 2, and a few group 3 isolates; nearly all Cluster B isolates were from group 6; other genotype groups were classified into Cluster C. Our newly developed MLVA-13Bc assay is a highly discriminatory assay for B. canis genotyping, and can serve as a useful molecular epidemiological tool, especially for tracing the source of contamination in an event of a B. canis outbreak.
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spelling pubmed-54308992017-05-16 Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay Yang, Yi Wang, Yin Poulsen, Elizabeth Ransburgh, Russell Liu, Xuming An, Baoyan Lu, Nanyan Anderson, Gary Wang, Chengming Bai, Jianfa Sci Rep Article Differentiation of Brucella canis from other Brucella species are mainly performed through PCR-based methods and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) procedures. Both PCR-based and MLVA methods are limited in discriminating B. canis strains. A new MLVA-13Bc method for B. canis genotyping was established by combining eight newly-developed VNTRs with five published ones. During 2010 and 2016, 377 B. canis PCR-positives were identified from 6,844 canine blood samples from 22 U.S. states, resulting in 229 B. canis isolates. The MLVA-13Bc method was able to differentiate each of these 229 isolates. The Hunter-Gaston Discriminatory Index of the individual VNTR loci ranged from 0.516 to 0.934 and the combined discriminatory index reached 1.000. Three major clusters (A, B and C) and 10 genotype groups were identified from the 229 B. canis isolates. Cluster A mainly contains genotype groups 1 and 2, and a few group 3 isolates; nearly all Cluster B isolates were from group 6; other genotype groups were classified into Cluster C. Our newly developed MLVA-13Bc assay is a highly discriminatory assay for B. canis genotyping, and can serve as a useful molecular epidemiological tool, especially for tracing the source of contamination in an event of a B. canis outbreak. Nature Publishing Group UK 2017-04-21 /pmc/articles/PMC5430899/ /pubmed/28432327 http://dx.doi.org/10.1038/s41598-017-01114-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yang, Yi
Wang, Yin
Poulsen, Elizabeth
Ransburgh, Russell
Liu, Xuming
An, Baoyan
Lu, Nanyan
Anderson, Gary
Wang, Chengming
Bai, Jianfa
Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay
title Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay
title_full Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay
title_fullStr Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay
title_full_unstemmed Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay
title_short Genotyping Brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (MLVA) assay
title_sort genotyping brucella canis isolates using a highly discriminatory multilocus variable-number tandem-repeat analysis (mlva) assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430899/
https://www.ncbi.nlm.nih.gov/pubmed/28432327
http://dx.doi.org/10.1038/s41598-017-01114-7
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