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Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients
Increased filaggrin expression was found to be correlated with severity scores in chronic spontaneous urticaria (CSU); however, the role of filaggrin breakdown products (FBPs) in CSU has not been studied. We collected stratum corneum (SC) specimens from the volar forearms of 10 CSU patients, 10 AD p...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431012/ https://www.ncbi.nlm.nih.gov/pubmed/28465560 http://dx.doi.org/10.1038/s41598-017-01487-9 |
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author | Pham, Duy Le Lim, Kyung-Min Joo, Kyung-Mi Park, Hae-Sim Leung, Donald Y. M. Ye, Young-Min |
author_facet | Pham, Duy Le Lim, Kyung-Min Joo, Kyung-Mi Park, Hae-Sim Leung, Donald Y. M. Ye, Young-Min |
author_sort | Pham, Duy Le |
collection | PubMed |
description | Increased filaggrin expression was found to be correlated with severity scores in chronic spontaneous urticaria (CSU); however, the role of filaggrin breakdown products (FBPs) in CSU has not been studied. We collected stratum corneum (SC) specimens from the volar forearms of 10 CSU patients, 10 AD patients, and 10 healthy normal controls (NCs) and measured contents of FBPs (pyrrolidone carboxylic acid [PCA] and urocanic acid [UCA]) using UPLC-MS/MS, transepidermal water loss (TEWL) and epidermal pH. Compared to NCs, cis-UCA level was increased in CSU lesions (P < 0.05) and decreased in AD lesions (P < 0.01). The cis-to-trans-UCA ratio in SC specimens from CSU patients was significantly greater than those from AD and NC subjects. AD lesions had lower FBP and PCA contents compared to NC skin (both P < 0.001), and higher TEWL and pH compared to CSU lesions. Moreover, cis-UCA, but not trans-UCA, enhanced the IgE-mediated basophil activation, as well as IgE- and calcium-mediated degranulation of LAD-2 cells, in a dose-dependent manner. These findings suggest that increased cis-to-trans UCA ratio in the epidermis is a distinct feature of CSU, which could enhance mast cell degranulation. Modulation of cis-UCA may be a potential target for skin diseases associated with IgE-mediated mast cell degranulation. |
format | Online Article Text |
id | pubmed-5431012 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54310122017-05-16 Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients Pham, Duy Le Lim, Kyung-Min Joo, Kyung-Mi Park, Hae-Sim Leung, Donald Y. M. Ye, Young-Min Sci Rep Article Increased filaggrin expression was found to be correlated with severity scores in chronic spontaneous urticaria (CSU); however, the role of filaggrin breakdown products (FBPs) in CSU has not been studied. We collected stratum corneum (SC) specimens from the volar forearms of 10 CSU patients, 10 AD patients, and 10 healthy normal controls (NCs) and measured contents of FBPs (pyrrolidone carboxylic acid [PCA] and urocanic acid [UCA]) using UPLC-MS/MS, transepidermal water loss (TEWL) and epidermal pH. Compared to NCs, cis-UCA level was increased in CSU lesions (P < 0.05) and decreased in AD lesions (P < 0.01). The cis-to-trans-UCA ratio in SC specimens from CSU patients was significantly greater than those from AD and NC subjects. AD lesions had lower FBP and PCA contents compared to NC skin (both P < 0.001), and higher TEWL and pH compared to CSU lesions. Moreover, cis-UCA, but not trans-UCA, enhanced the IgE-mediated basophil activation, as well as IgE- and calcium-mediated degranulation of LAD-2 cells, in a dose-dependent manner. These findings suggest that increased cis-to-trans UCA ratio in the epidermis is a distinct feature of CSU, which could enhance mast cell degranulation. Modulation of cis-UCA may be a potential target for skin diseases associated with IgE-mediated mast cell degranulation. Nature Publishing Group UK 2017-05-02 /pmc/articles/PMC5431012/ /pubmed/28465560 http://dx.doi.org/10.1038/s41598-017-01487-9 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Pham, Duy Le Lim, Kyung-Min Joo, Kyung-Mi Park, Hae-Sim Leung, Donald Y. M. Ye, Young-Min Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients |
title | Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients |
title_full | Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients |
title_fullStr | Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients |
title_full_unstemmed | Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients |
title_short | Increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients |
title_sort | increased cis-to-trans urocanic acid ratio in the skin of chronic spontaneous urticaria patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431012/ https://www.ncbi.nlm.nih.gov/pubmed/28465560 http://dx.doi.org/10.1038/s41598-017-01487-9 |
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