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Nerve growth factor signalling in pathology and regeneration of human teeth

Nerve growth factor (NGF) is a key regulator of the development and differentiation of neuronal and non-neuronal cells. In the present study we examined the distribution of NGF and its low and high-affinity receptors, p75(NTR) and TrkA respectively, in permanent human teeth under normal and patholog...

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Autores principales: Mitsiadis, Thimios A., Magloire, Henry, Pagella, Pierfrancesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431060/
https://www.ncbi.nlm.nih.gov/pubmed/28465581
http://dx.doi.org/10.1038/s41598-017-01455-3
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author Mitsiadis, Thimios A.
Magloire, Henry
Pagella, Pierfrancesco
author_facet Mitsiadis, Thimios A.
Magloire, Henry
Pagella, Pierfrancesco
author_sort Mitsiadis, Thimios A.
collection PubMed
description Nerve growth factor (NGF) is a key regulator of the development and differentiation of neuronal and non-neuronal cells. In the present study we examined the distribution of NGF and its low and high-affinity receptors, p75(NTR) and TrkA respectively, in permanent human teeth under normal and pathological conditions. In intact functional teeth, NGF, p75(NTR) and TrkA are weakly expressed in dental pulp fibroblasts and odontoblasts that are responsible for dentine formation, while the NGF and p75(NTR) molecules are strongly expressed in nerve fibres innervating the dental pulp. In carious and injured teeth NGF and TrkA expression is upregulated in a selective manner in odontoblasts surrounding the injury sites, indicating a link between NGF signalling and dental tissue repair events. Accordingly, NGF and TrkA expression is strongly upregulated in cultured primary human dental mesenchymal cells during their differentiation into odontoblasts. Targeted release of NGF in cultured human tooth slices induced extensive axonal growth and migration of Schwann cells towards the NGF administration site. These results show that NGF signalling is strongly linked to pathological and regenerative processes in human teeth and suggest a potential role for this neurotrophic molecule in pulp regeneration.
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spelling pubmed-54310602017-05-16 Nerve growth factor signalling in pathology and regeneration of human teeth Mitsiadis, Thimios A. Magloire, Henry Pagella, Pierfrancesco Sci Rep Article Nerve growth factor (NGF) is a key regulator of the development and differentiation of neuronal and non-neuronal cells. In the present study we examined the distribution of NGF and its low and high-affinity receptors, p75(NTR) and TrkA respectively, in permanent human teeth under normal and pathological conditions. In intact functional teeth, NGF, p75(NTR) and TrkA are weakly expressed in dental pulp fibroblasts and odontoblasts that are responsible for dentine formation, while the NGF and p75(NTR) molecules are strongly expressed in nerve fibres innervating the dental pulp. In carious and injured teeth NGF and TrkA expression is upregulated in a selective manner in odontoblasts surrounding the injury sites, indicating a link between NGF signalling and dental tissue repair events. Accordingly, NGF and TrkA expression is strongly upregulated in cultured primary human dental mesenchymal cells during their differentiation into odontoblasts. Targeted release of NGF in cultured human tooth slices induced extensive axonal growth and migration of Schwann cells towards the NGF administration site. These results show that NGF signalling is strongly linked to pathological and regenerative processes in human teeth and suggest a potential role for this neurotrophic molecule in pulp regeneration. Nature Publishing Group UK 2017-05-02 /pmc/articles/PMC5431060/ /pubmed/28465581 http://dx.doi.org/10.1038/s41598-017-01455-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mitsiadis, Thimios A.
Magloire, Henry
Pagella, Pierfrancesco
Nerve growth factor signalling in pathology and regeneration of human teeth
title Nerve growth factor signalling in pathology and regeneration of human teeth
title_full Nerve growth factor signalling in pathology and regeneration of human teeth
title_fullStr Nerve growth factor signalling in pathology and regeneration of human teeth
title_full_unstemmed Nerve growth factor signalling in pathology and regeneration of human teeth
title_short Nerve growth factor signalling in pathology and regeneration of human teeth
title_sort nerve growth factor signalling in pathology and regeneration of human teeth
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431060/
https://www.ncbi.nlm.nih.gov/pubmed/28465581
http://dx.doi.org/10.1038/s41598-017-01455-3
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