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Alumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation
We present a high-yield method for the renaturation of negatively charged enzymes. The approach is based on the use of alumina nanoparticles, which after electrostatic interaction with denatured protein molecules, prevent their aggregation and make the process of refolding controllable. The method,...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431136/ https://www.ncbi.nlm.nih.gov/pubmed/28469182 http://dx.doi.org/10.1038/s41598-017-01436-6 |