Integrated single- and two-photon light sheet microscopy using accelerating beams

We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an i...

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Autores principales: Piksarv, Peeter, Marti, Dominik, Le, Tuan, Unterhuber, Angelika, Forbes, Lindsey H., Andrews, Melissa R., Stingl, Andreas, Drexler, Wolfgang, Andersen, Peter E., Dholakia, Kishan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431168/
https://www.ncbi.nlm.nih.gov/pubmed/28469191
http://dx.doi.org/10.1038/s41598-017-01543-4
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author Piksarv, Peeter
Marti, Dominik
Le, Tuan
Unterhuber, Angelika
Forbes, Lindsey H.
Andrews, Melissa R.
Stingl, Andreas
Drexler, Wolfgang
Andersen, Peter E.
Dholakia, Kishan
author_facet Piksarv, Peeter
Marti, Dominik
Le, Tuan
Unterhuber, Angelika
Forbes, Lindsey H.
Andrews, Melissa R.
Stingl, Andreas
Drexler, Wolfgang
Andersen, Peter E.
Dholakia, Kishan
author_sort Piksarv, Peeter
collection PubMed
description We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.
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spelling pubmed-54311682017-05-16 Integrated single- and two-photon light sheet microscopy using accelerating beams Piksarv, Peeter Marti, Dominik Le, Tuan Unterhuber, Angelika Forbes, Lindsey H. Andrews, Melissa R. Stingl, Andreas Drexler, Wolfgang Andersen, Peter E. Dholakia, Kishan Sci Rep Article We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes. Nature Publishing Group UK 2017-05-03 /pmc/articles/PMC5431168/ /pubmed/28469191 http://dx.doi.org/10.1038/s41598-017-01543-4 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Piksarv, Peeter
Marti, Dominik
Le, Tuan
Unterhuber, Angelika
Forbes, Lindsey H.
Andrews, Melissa R.
Stingl, Andreas
Drexler, Wolfgang
Andersen, Peter E.
Dholakia, Kishan
Integrated single- and two-photon light sheet microscopy using accelerating beams
title Integrated single- and two-photon light sheet microscopy using accelerating beams
title_full Integrated single- and two-photon light sheet microscopy using accelerating beams
title_fullStr Integrated single- and two-photon light sheet microscopy using accelerating beams
title_full_unstemmed Integrated single- and two-photon light sheet microscopy using accelerating beams
title_short Integrated single- and two-photon light sheet microscopy using accelerating beams
title_sort integrated single- and two-photon light sheet microscopy using accelerating beams
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431168/
https://www.ncbi.nlm.nih.gov/pubmed/28469191
http://dx.doi.org/10.1038/s41598-017-01543-4
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