Cargando…
The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis
ChHP1BP3, a chromatin complex-related protein known with dynamic features, was identified as a ChHsp70 promoter-associated factor in Crassostrea hongkongensis by DNA-affinity purification and mass spectrometry analysis. Direct interaction between purified ChHP1BP3 and the ChHsp70 promoter region was...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431216/ https://www.ncbi.nlm.nih.gov/pubmed/28469151 http://dx.doi.org/10.1038/s41598-017-01573-y |
_version_ | 1783236391315963904 |
---|---|
author | Xu, Delin Yang, Qin Cui, Miao Zhang, Qizhong |
author_facet | Xu, Delin Yang, Qin Cui, Miao Zhang, Qizhong |
author_sort | Xu, Delin |
collection | PubMed |
description | ChHP1BP3, a chromatin complex-related protein known with dynamic features, was identified as a ChHsp70 promoter-associated factor in Crassostrea hongkongensis by DNA-affinity purification and mass spectrometry analysis. Direct interaction between purified ChHP1BP3 and the ChHsp70 promoter region was demonstrated using EMSA. ChHp1bp3 depletion led to clear enhancements in ChHsp70 mRNA expression in C. hongkongensis hemocytes. However, ChHp1bp3 overexpression in heterologous HEK293T cells correlated with fluctuations in ChHsp70 transcription. Quantitative RT-PCR analysis showed that both ChHsp70 and ChHp1bp3 transcription were responsive to external physical/chemical stresses by heat, CdCl(2) and NP. This indicated a plausible correlation between ChHsp70 and ChHp1bp3 in the stress-induced genetic regulatory pathway. While, the distinctive ChHp1bp3 expression patterns upon physical and chemical stresses suggest that the mechanisms that mediate ChHp1bp3 induction might be stress-specific. This study discovered a novel role for HP1BP3 as a negative regulator in controlling Hsp70 transcription in C. hongkongensis, and contributed to better understanding the complex regulatory mechanisms governing Hsp70 transcription. |
format | Online Article Text |
id | pubmed-5431216 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54312162017-05-16 The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis Xu, Delin Yang, Qin Cui, Miao Zhang, Qizhong Sci Rep Article ChHP1BP3, a chromatin complex-related protein known with dynamic features, was identified as a ChHsp70 promoter-associated factor in Crassostrea hongkongensis by DNA-affinity purification and mass spectrometry analysis. Direct interaction between purified ChHP1BP3 and the ChHsp70 promoter region was demonstrated using EMSA. ChHp1bp3 depletion led to clear enhancements in ChHsp70 mRNA expression in C. hongkongensis hemocytes. However, ChHp1bp3 overexpression in heterologous HEK293T cells correlated with fluctuations in ChHsp70 transcription. Quantitative RT-PCR analysis showed that both ChHsp70 and ChHp1bp3 transcription were responsive to external physical/chemical stresses by heat, CdCl(2) and NP. This indicated a plausible correlation between ChHsp70 and ChHp1bp3 in the stress-induced genetic regulatory pathway. While, the distinctive ChHp1bp3 expression patterns upon physical and chemical stresses suggest that the mechanisms that mediate ChHp1bp3 induction might be stress-specific. This study discovered a novel role for HP1BP3 as a negative regulator in controlling Hsp70 transcription in C. hongkongensis, and contributed to better understanding the complex regulatory mechanisms governing Hsp70 transcription. Nature Publishing Group UK 2017-05-03 /pmc/articles/PMC5431216/ /pubmed/28469151 http://dx.doi.org/10.1038/s41598-017-01573-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Xu, Delin Yang, Qin Cui, Miao Zhang, Qizhong The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis |
title | The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis |
title_full | The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis |
title_fullStr | The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis |
title_full_unstemmed | The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis |
title_short | The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis |
title_sort | novel transcriptional factor hp1bp3 negatively regulates hsp70 transcription in crassostrea hongkongensis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431216/ https://www.ncbi.nlm.nih.gov/pubmed/28469151 http://dx.doi.org/10.1038/s41598-017-01573-y |
work_keys_str_mv | AT xudelin thenoveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT yangqin thenoveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT cuimiao thenoveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT zhangqizhong thenoveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT xudelin noveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT yangqin noveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT cuimiao noveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis AT zhangqizhong noveltranscriptionalfactorhp1bp3negativelyregulateshsp70transcriptionincrassostreahongkongensis |