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mRNA expression of steroidogenic enzymes, steroid hormone receptors and their coregulators in gastric cancer
Epidemiological and experimental findings suggest that the development of gastric cancer (GC) is regulated by steroid hormones. In postmenopausal women and older men, the majority of steroid hormones are produced locally in peripheral tissue through the enzymatic conversion of steroid precursors. Th...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431337/ https://www.ncbi.nlm.nih.gov/pubmed/28521442 http://dx.doi.org/10.3892/ol.2017.5881 |
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author | Frycz, Bartosz Adam Murawa, Dawid Borejsza-Wysocki, Maciej Wichtowski, Mateusz Spychała, Arkadiusz Marciniak, Ryszard Murawa, Paweł Drews, Michał Jagodziński, Paweł Piotr |
author_facet | Frycz, Bartosz Adam Murawa, Dawid Borejsza-Wysocki, Maciej Wichtowski, Mateusz Spychała, Arkadiusz Marciniak, Ryszard Murawa, Paweł Drews, Michał Jagodziński, Paweł Piotr |
author_sort | Frycz, Bartosz Adam |
collection | PubMed |
description | Epidemiological and experimental findings suggest that the development of gastric cancer (GC) is regulated by steroid hormones. In postmenopausal women and older men, the majority of steroid hormones are produced locally in peripheral tissue through the enzymatic conversion of steroid precursors. Therefore, using reverse transcription-quantitative polymerase chain reaction analysis, the mRNA expression of genes encoding steroidogenic enzymes, including steroid sulfatase (STS), hydroxy-delta-5-steroid dehydrogenase 3 beta- and steroid delta-isomerase 1 (HSD3B1), 17β-hydroxysteroid dehydrogenase type 7 and aromatase (CYP19A1), was investigated in primary tumoral and adjacent healthy gastric mucosa from 60 patients with GC. Furthermore, the mRNA levels for estrogen receptor α, estrogen receptor β (ESR2) and androgen receptor (AR), along with their coregulators, including proline, glutamate and leucine rich protein 1, CREB binding protein, nuclear receptor coactivator 1 (NCOA1), nuclear receptor corepressor 1 (NCOR1) and nuclear receptor subfamily 2 group F member 1 (NR2F1), were investigated. Additionally, the association between the mRNA expression of these genes and the clinicopathological features of patients with GC was examined. Significantly decreased levels of STS, HSD3B1, ESR2, AR, NCOA1 and NCOR1 mRNA, in addition to significantly increased levels of CYP19A1 mRNA were demonstrated in tumoral tissue samples compared with adjacent healthy gastric tissue samples. Deregulated expression of these genes in the analyzed tissue samples was associated with certain clinicopathological features of GC, such as age and localization of the tumor. The results of the current study suggest that all of the genes analyzed are expressed in tumoral and adjacent healthy gastric mucosa. In addition, the results indicate that abnormal expression of STS, ESR2, AR, NCOA1 and NCOR1 may serve a role in the development and progression of GC, and may be associated with specific clinicopathological features in patients with GC. |
format | Online Article Text |
id | pubmed-5431337 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-54313372017-05-17 mRNA expression of steroidogenic enzymes, steroid hormone receptors and their coregulators in gastric cancer Frycz, Bartosz Adam Murawa, Dawid Borejsza-Wysocki, Maciej Wichtowski, Mateusz Spychała, Arkadiusz Marciniak, Ryszard Murawa, Paweł Drews, Michał Jagodziński, Paweł Piotr Oncol Lett Articles Epidemiological and experimental findings suggest that the development of gastric cancer (GC) is regulated by steroid hormones. In postmenopausal women and older men, the majority of steroid hormones are produced locally in peripheral tissue through the enzymatic conversion of steroid precursors. Therefore, using reverse transcription-quantitative polymerase chain reaction analysis, the mRNA expression of genes encoding steroidogenic enzymes, including steroid sulfatase (STS), hydroxy-delta-5-steroid dehydrogenase 3 beta- and steroid delta-isomerase 1 (HSD3B1), 17β-hydroxysteroid dehydrogenase type 7 and aromatase (CYP19A1), was investigated in primary tumoral and adjacent healthy gastric mucosa from 60 patients with GC. Furthermore, the mRNA levels for estrogen receptor α, estrogen receptor β (ESR2) and androgen receptor (AR), along with their coregulators, including proline, glutamate and leucine rich protein 1, CREB binding protein, nuclear receptor coactivator 1 (NCOA1), nuclear receptor corepressor 1 (NCOR1) and nuclear receptor subfamily 2 group F member 1 (NR2F1), were investigated. Additionally, the association between the mRNA expression of these genes and the clinicopathological features of patients with GC was examined. Significantly decreased levels of STS, HSD3B1, ESR2, AR, NCOA1 and NCOR1 mRNA, in addition to significantly increased levels of CYP19A1 mRNA were demonstrated in tumoral tissue samples compared with adjacent healthy gastric tissue samples. Deregulated expression of these genes in the analyzed tissue samples was associated with certain clinicopathological features of GC, such as age and localization of the tumor. The results of the current study suggest that all of the genes analyzed are expressed in tumoral and adjacent healthy gastric mucosa. In addition, the results indicate that abnormal expression of STS, ESR2, AR, NCOA1 and NCOR1 may serve a role in the development and progression of GC, and may be associated with specific clinicopathological features in patients with GC. D.A. Spandidos 2017-05 2017-03-21 /pmc/articles/PMC5431337/ /pubmed/28521442 http://dx.doi.org/10.3892/ol.2017.5881 Text en Copyright: © Frycz et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Frycz, Bartosz Adam Murawa, Dawid Borejsza-Wysocki, Maciej Wichtowski, Mateusz Spychała, Arkadiusz Marciniak, Ryszard Murawa, Paweł Drews, Michał Jagodziński, Paweł Piotr mRNA expression of steroidogenic enzymes, steroid hormone receptors and their coregulators in gastric cancer |
title | mRNA expression of steroidogenic enzymes, steroid hormone receptors and their coregulators in gastric cancer |
title_full | mRNA expression of steroidogenic enzymes, steroid hormone receptors and their coregulators in gastric cancer |
title_fullStr | mRNA expression of steroidogenic enzymes, steroid hormone receptors and their coregulators in gastric cancer |
title_full_unstemmed | mRNA expression of steroidogenic enzymes, steroid hormone receptors and their coregulators in gastric cancer |
title_short | mRNA expression of steroidogenic enzymes, steroid hormone receptors and their coregulators in gastric cancer |
title_sort | mrna expression of steroidogenic enzymes, steroid hormone receptors and their coregulators in gastric cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431337/ https://www.ncbi.nlm.nih.gov/pubmed/28521442 http://dx.doi.org/10.3892/ol.2017.5881 |
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