Generation of mutation hotspots in ageing bacterial colonies

How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: mo...

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Autores principales: Sekowska, Agnieszka, Wendel, Sofie, Fischer, Emil C., Nørholm, Morten H. H., Danchin, Antoine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431349/
https://www.ncbi.nlm.nih.gov/pubmed/28442761
http://dx.doi.org/10.1038/s41598-016-0005-4
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author Sekowska, Agnieszka
Wendel, Sofie
Fischer, Emil C.
Nørholm, Morten H. H.
Danchin, Antoine
author_facet Sekowska, Agnieszka
Wendel, Sofie
Fischer, Emil C.
Nørholm, Morten H. H.
Danchin, Antoine
author_sort Sekowska, Agnieszka
collection PubMed
description How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.
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spelling pubmed-54313492017-05-17 Generation of mutation hotspots in ageing bacterial colonies Sekowska, Agnieszka Wendel, Sofie Fischer, Emil C. Nørholm, Morten H. H. Danchin, Antoine Sci Rep Article How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing. Nature Publishing Group UK 2016-12-05 /pmc/articles/PMC5431349/ /pubmed/28442761 http://dx.doi.org/10.1038/s41598-016-0005-4 Text en © The Author(s) 2016 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Sekowska, Agnieszka
Wendel, Sofie
Fischer, Emil C.
Nørholm, Morten H. H.
Danchin, Antoine
Generation of mutation hotspots in ageing bacterial colonies
title Generation of mutation hotspots in ageing bacterial colonies
title_full Generation of mutation hotspots in ageing bacterial colonies
title_fullStr Generation of mutation hotspots in ageing bacterial colonies
title_full_unstemmed Generation of mutation hotspots in ageing bacterial colonies
title_short Generation of mutation hotspots in ageing bacterial colonies
title_sort generation of mutation hotspots in ageing bacterial colonies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431349/
https://www.ncbi.nlm.nih.gov/pubmed/28442761
http://dx.doi.org/10.1038/s41598-016-0005-4
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