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miR-195 inhibits cell proliferation via targeting AEG-1 in hepatocellular carcinoma

Emerging evidence has indicated that microRNAs (miRNAs) are frequently dysregulated and are fundamental in the pathogenesis of hepatocellular carcinoma (HCC). However, the roles of miR-195 in HCC have not been well elucidated. In the present study, the expression of miR-195 was determined to be mark...

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Autores principales: Yan, Jing-Jun, Chang, Ying, Zhang, Yu-Nan, Lin, Ju-Sheng, He, Xing-Xing, Huang, Huan-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431445/
https://www.ncbi.nlm.nih.gov/pubmed/28529562
http://dx.doi.org/10.3892/ol.2017.5826
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author Yan, Jing-Jun
Chang, Ying
Zhang, Yu-Nan
Lin, Ju-Sheng
He, Xing-Xing
Huang, Huan-Jun
author_facet Yan, Jing-Jun
Chang, Ying
Zhang, Yu-Nan
Lin, Ju-Sheng
He, Xing-Xing
Huang, Huan-Jun
author_sort Yan, Jing-Jun
collection PubMed
description Emerging evidence has indicated that microRNAs (miRNAs) are frequently dysregulated and are fundamental in the pathogenesis of hepatocellular carcinoma (HCC). However, the roles of miR-195 in HCC have not been well elucidated. In the present study, the expression of miR-195 was determined to be markedly downregulated in HCC tissues and cell lines, as compared with normal liver cells. Restoration of miR-195 expression resulted in significant inhibition of the proliferation and tumorigenicity of HCC cells in vitro and in vivo. Gene expression data and luciferase reporter assays revealed that miR-195 is able to directly inhibit the expression of astrocyte elevated gene 1 (AEG-1) through interaction with its 3′ untranslated region. Consistently, an inverse correlation between miR-195 and AEG-1 expression was observed in HCC tissues. Furthermore, the overexpression of AEG-1 was able to partially attenuate the miR-195-induced inhibition of cell growth and promotion of apoptosis. Taken together, these findings indicate that miR-195 functions as a tumor suppressor by inhibiting AEG-1. This pathway may provide new insights into the potential molecular mechanisms of HCC.
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spelling pubmed-54314452017-05-19 miR-195 inhibits cell proliferation via targeting AEG-1 in hepatocellular carcinoma Yan, Jing-Jun Chang, Ying Zhang, Yu-Nan Lin, Ju-Sheng He, Xing-Xing Huang, Huan-Jun Oncol Lett Articles Emerging evidence has indicated that microRNAs (miRNAs) are frequently dysregulated and are fundamental in the pathogenesis of hepatocellular carcinoma (HCC). However, the roles of miR-195 in HCC have not been well elucidated. In the present study, the expression of miR-195 was determined to be markedly downregulated in HCC tissues and cell lines, as compared with normal liver cells. Restoration of miR-195 expression resulted in significant inhibition of the proliferation and tumorigenicity of HCC cells in vitro and in vivo. Gene expression data and luciferase reporter assays revealed that miR-195 is able to directly inhibit the expression of astrocyte elevated gene 1 (AEG-1) through interaction with its 3′ untranslated region. Consistently, an inverse correlation between miR-195 and AEG-1 expression was observed in HCC tissues. Furthermore, the overexpression of AEG-1 was able to partially attenuate the miR-195-induced inhibition of cell growth and promotion of apoptosis. Taken together, these findings indicate that miR-195 functions as a tumor suppressor by inhibiting AEG-1. This pathway may provide new insights into the potential molecular mechanisms of HCC. D.A. Spandidos 2017-05 2017-03-08 /pmc/articles/PMC5431445/ /pubmed/28529562 http://dx.doi.org/10.3892/ol.2017.5826 Text en Copyright: © Yan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Yan, Jing-Jun
Chang, Ying
Zhang, Yu-Nan
Lin, Ju-Sheng
He, Xing-Xing
Huang, Huan-Jun
miR-195 inhibits cell proliferation via targeting AEG-1 in hepatocellular carcinoma
title miR-195 inhibits cell proliferation via targeting AEG-1 in hepatocellular carcinoma
title_full miR-195 inhibits cell proliferation via targeting AEG-1 in hepatocellular carcinoma
title_fullStr miR-195 inhibits cell proliferation via targeting AEG-1 in hepatocellular carcinoma
title_full_unstemmed miR-195 inhibits cell proliferation via targeting AEG-1 in hepatocellular carcinoma
title_short miR-195 inhibits cell proliferation via targeting AEG-1 in hepatocellular carcinoma
title_sort mir-195 inhibits cell proliferation via targeting aeg-1 in hepatocellular carcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431445/
https://www.ncbi.nlm.nih.gov/pubmed/28529562
http://dx.doi.org/10.3892/ol.2017.5826
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