Cargando…

The physiological and molecular response of Aurelia sp.1 under hypoxia

Few studies have been published on the mechanisms of hypoxia response and tolerance in jellyfish, especially with respect to the regulatory mechanism at the molecular level. In this study, Aurelia sp.1, which is frequently found in Chinese coastal waters, was cultivated in a hypoxic system to determ...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Guoshan, Zhen, Yu, Yu, Zhigang, Shi, Yan, Zhao, Qing, Wang, Jianyan, Mi, Tiezhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431473/
https://www.ncbi.nlm.nih.gov/pubmed/28484259
http://dx.doi.org/10.1038/s41598-017-01318-x
_version_ 1783236433233838080
author Wang, Guoshan
Zhen, Yu
Yu, Zhigang
Shi, Yan
Zhao, Qing
Wang, Jianyan
Mi, Tiezhu
author_facet Wang, Guoshan
Zhen, Yu
Yu, Zhigang
Shi, Yan
Zhao, Qing
Wang, Jianyan
Mi, Tiezhu
author_sort Wang, Guoshan
collection PubMed
description Few studies have been published on the mechanisms of hypoxia response and tolerance in jellyfish, especially with respect to the regulatory mechanism at the molecular level. In this study, Aurelia sp.1, which is frequently found in Chinese coastal waters, was cultivated in a hypoxic system to determine the molecular mechanisms underlying its hypoxic response by studying the physiological activity, gene expression and metabolite contents in the prolyl hydroxylase domain (PHD)-hypoxia inducible factor (HIF) oxygen-sensing system. Physiological activity; the expression of PHD, HIF, ALDO (fructose-bisphosphate aldolase), PDK (pyruvate dehydrogenase kinase), and LDH (lactate dehydrogenase) genes; and the lactic acid content in medusae were significantly affected by hypoxia. The up-regulation of ALDO, PDK and LDH, which was directly or indirectly induced by HIF, mediated the transition from aerobic respiration to anaerobic glycolysis in the medusae. In polyps, there was a slight increase in the expression of HIF, PHD and ALDO, no obvious change in that of PDK and a slight decrease in that of LDH throughout the experiment; however, these changes were insufficient to induce the shift. This study provides a scientific basis for elucidating the regulatory mechanism underlying the PHD-HIF oxygen-sensing system in Aurelia sp.1.
format Online
Article
Text
id pubmed-5431473
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-54314732017-05-16 The physiological and molecular response of Aurelia sp.1 under hypoxia Wang, Guoshan Zhen, Yu Yu, Zhigang Shi, Yan Zhao, Qing Wang, Jianyan Mi, Tiezhu Sci Rep Article Few studies have been published on the mechanisms of hypoxia response and tolerance in jellyfish, especially with respect to the regulatory mechanism at the molecular level. In this study, Aurelia sp.1, which is frequently found in Chinese coastal waters, was cultivated in a hypoxic system to determine the molecular mechanisms underlying its hypoxic response by studying the physiological activity, gene expression and metabolite contents in the prolyl hydroxylase domain (PHD)-hypoxia inducible factor (HIF) oxygen-sensing system. Physiological activity; the expression of PHD, HIF, ALDO (fructose-bisphosphate aldolase), PDK (pyruvate dehydrogenase kinase), and LDH (lactate dehydrogenase) genes; and the lactic acid content in medusae were significantly affected by hypoxia. The up-regulation of ALDO, PDK and LDH, which was directly or indirectly induced by HIF, mediated the transition from aerobic respiration to anaerobic glycolysis in the medusae. In polyps, there was a slight increase in the expression of HIF, PHD and ALDO, no obvious change in that of PDK and a slight decrease in that of LDH throughout the experiment; however, these changes were insufficient to induce the shift. This study provides a scientific basis for elucidating the regulatory mechanism underlying the PHD-HIF oxygen-sensing system in Aurelia sp.1. Nature Publishing Group UK 2017-05-08 /pmc/articles/PMC5431473/ /pubmed/28484259 http://dx.doi.org/10.1038/s41598-017-01318-x Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Guoshan
Zhen, Yu
Yu, Zhigang
Shi, Yan
Zhao, Qing
Wang, Jianyan
Mi, Tiezhu
The physiological and molecular response of Aurelia sp.1 under hypoxia
title The physiological and molecular response of Aurelia sp.1 under hypoxia
title_full The physiological and molecular response of Aurelia sp.1 under hypoxia
title_fullStr The physiological and molecular response of Aurelia sp.1 under hypoxia
title_full_unstemmed The physiological and molecular response of Aurelia sp.1 under hypoxia
title_short The physiological and molecular response of Aurelia sp.1 under hypoxia
title_sort physiological and molecular response of aurelia sp.1 under hypoxia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431473/
https://www.ncbi.nlm.nih.gov/pubmed/28484259
http://dx.doi.org/10.1038/s41598-017-01318-x
work_keys_str_mv AT wangguoshan thephysiologicalandmolecularresponseofaureliasp1underhypoxia
AT zhenyu thephysiologicalandmolecularresponseofaureliasp1underhypoxia
AT yuzhigang thephysiologicalandmolecularresponseofaureliasp1underhypoxia
AT shiyan thephysiologicalandmolecularresponseofaureliasp1underhypoxia
AT zhaoqing thephysiologicalandmolecularresponseofaureliasp1underhypoxia
AT wangjianyan thephysiologicalandmolecularresponseofaureliasp1underhypoxia
AT mitiezhu thephysiologicalandmolecularresponseofaureliasp1underhypoxia
AT wangguoshan physiologicalandmolecularresponseofaureliasp1underhypoxia
AT zhenyu physiologicalandmolecularresponseofaureliasp1underhypoxia
AT yuzhigang physiologicalandmolecularresponseofaureliasp1underhypoxia
AT shiyan physiologicalandmolecularresponseofaureliasp1underhypoxia
AT zhaoqing physiologicalandmolecularresponseofaureliasp1underhypoxia
AT wangjianyan physiologicalandmolecularresponseofaureliasp1underhypoxia
AT mitiezhu physiologicalandmolecularresponseofaureliasp1underhypoxia