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Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor

Estimation of postmortem interval (PMI) is a central role in medico-legal identification. Analysis of vitreous potassium ions (K(+)) concentration is frequently used by forensic workers to estimate PMI. This paper describes interdisciplinary research to introduce fluorescence sensing techniques into...

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Autores principales: Ding, Yanjun, Li, Xingmei, Guo, Yadong, Duan, Weicheng, Ling, Jiang, Zha, Lagabaiyla, Yan, Jie, Zou, Ying, Cai, Jifeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431803/
https://www.ncbi.nlm.nih.gov/pubmed/28500353
http://dx.doi.org/10.1038/s41598-017-02027-1
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author Ding, Yanjun
Li, Xingmei
Guo, Yadong
Duan, Weicheng
Ling, Jiang
Zha, Lagabaiyla
Yan, Jie
Zou, Ying
Cai, Jifeng
author_facet Ding, Yanjun
Li, Xingmei
Guo, Yadong
Duan, Weicheng
Ling, Jiang
Zha, Lagabaiyla
Yan, Jie
Zou, Ying
Cai, Jifeng
author_sort Ding, Yanjun
collection PubMed
description Estimation of postmortem interval (PMI) is a central role in medico-legal identification. Analysis of vitreous potassium ions (K(+)) concentration is frequently used by forensic workers to estimate PMI. This paper describes interdisciplinary research to introduce fluorescence sensing techniques into forensic medicine. On the basis of silver nanoclusters (AgNCs) probe stabilized by DNA, a simple and highly sensitive fluorescence aptasensor has been proposed to selectively detect K(+) ions. The linear range for K(+) ions was found to be 0.1 nM-1 mM, with limit of detection of 0.06 nM. Moreover, 63 vitreous humour cases within 36 h after death were further studied to verify the utility of K(+) ions in estimating the PMI. By the fluorescence aptasensor method, a new formula was built to determine the postmortem interval based on K(+) ions concentration: PMI(h) = −0.55 + 1.66 × C(K) (+)(r = 0.791). And the real significance of this research was demonstrated by additional 6 cases with known PMIs. In comparison with the conventional method, the presented aptasensor strategy is cost-effective and easy in measuring vitreous K(+), which may be potentially a better way for estimation of PMI in medico-legal practice.
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spelling pubmed-54318032017-05-16 Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor Ding, Yanjun Li, Xingmei Guo, Yadong Duan, Weicheng Ling, Jiang Zha, Lagabaiyla Yan, Jie Zou, Ying Cai, Jifeng Sci Rep Article Estimation of postmortem interval (PMI) is a central role in medico-legal identification. Analysis of vitreous potassium ions (K(+)) concentration is frequently used by forensic workers to estimate PMI. This paper describes interdisciplinary research to introduce fluorescence sensing techniques into forensic medicine. On the basis of silver nanoclusters (AgNCs) probe stabilized by DNA, a simple and highly sensitive fluorescence aptasensor has been proposed to selectively detect K(+) ions. The linear range for K(+) ions was found to be 0.1 nM-1 mM, with limit of detection of 0.06 nM. Moreover, 63 vitreous humour cases within 36 h after death were further studied to verify the utility of K(+) ions in estimating the PMI. By the fluorescence aptasensor method, a new formula was built to determine the postmortem interval based on K(+) ions concentration: PMI(h) = −0.55 + 1.66 × C(K) (+)(r = 0.791). And the real significance of this research was demonstrated by additional 6 cases with known PMIs. In comparison with the conventional method, the presented aptasensor strategy is cost-effective and easy in measuring vitreous K(+), which may be potentially a better way for estimation of PMI in medico-legal practice. Nature Publishing Group UK 2017-05-12 /pmc/articles/PMC5431803/ /pubmed/28500353 http://dx.doi.org/10.1038/s41598-017-02027-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ding, Yanjun
Li, Xingmei
Guo, Yadong
Duan, Weicheng
Ling, Jiang
Zha, Lagabaiyla
Yan, Jie
Zou, Ying
Cai, Jifeng
Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
title Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
title_full Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
title_fullStr Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
title_full_unstemmed Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
title_short Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
title_sort estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431803/
https://www.ncbi.nlm.nih.gov/pubmed/28500353
http://dx.doi.org/10.1038/s41598-017-02027-1
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