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Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer
Ribosome inactivating proteins (RIPs) are RNA N-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28S rRNA. These enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. Recently, we reported for...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431988/ https://www.ncbi.nlm.nih.gov/pubmed/28500327 http://dx.doi.org/10.1038/s41598-017-01859-1 |
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author | Lapadula, Walter J. Marcet, Paula L. Mascotti, María L. Sanchez-Puerta, M. Virginia Juri Ayub, Maximiliano |
author_facet | Lapadula, Walter J. Marcet, Paula L. Mascotti, María L. Sanchez-Puerta, M. Virginia Juri Ayub, Maximiliano |
author_sort | Lapadula, Walter J. |
collection | PubMed |
description | Ribosome inactivating proteins (RIPs) are RNA N-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28S rRNA. These enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. Recently, we reported for the first time in silico evidence of RIP encoding genes in metazoans, in two closely related species of insects: Aedes aegypti and Culex quinquefasciatus. Here, we have experimentally confirmed the presence of these genes in mosquitoes and attempted to unveil their evolutionary history. A detailed study was conducted, including evaluation of taxonomic distribution, phylogenetic inferences and microsynteny analyses, indicating that mosquito RIP genes derived from a single Horizontal Gene Transfer (HGT) event, probably from a cyanobacterial donor species. Moreover, evolutionary analyses show that, after the HGT event, these genes evolved under purifying selection, strongly suggesting they play functional roles in these organisms. |
format | Online Article Text |
id | pubmed-5431988 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54319882017-05-16 Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer Lapadula, Walter J. Marcet, Paula L. Mascotti, María L. Sanchez-Puerta, M. Virginia Juri Ayub, Maximiliano Sci Rep Article Ribosome inactivating proteins (RIPs) are RNA N-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28S rRNA. These enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. Recently, we reported for the first time in silico evidence of RIP encoding genes in metazoans, in two closely related species of insects: Aedes aegypti and Culex quinquefasciatus. Here, we have experimentally confirmed the presence of these genes in mosquitoes and attempted to unveil their evolutionary history. A detailed study was conducted, including evaluation of taxonomic distribution, phylogenetic inferences and microsynteny analyses, indicating that mosquito RIP genes derived from a single Horizontal Gene Transfer (HGT) event, probably from a cyanobacterial donor species. Moreover, evolutionary analyses show that, after the HGT event, these genes evolved under purifying selection, strongly suggesting they play functional roles in these organisms. Nature Publishing Group UK 2017-05-12 /pmc/articles/PMC5431988/ /pubmed/28500327 http://dx.doi.org/10.1038/s41598-017-01859-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lapadula, Walter J. Marcet, Paula L. Mascotti, María L. Sanchez-Puerta, M. Virginia Juri Ayub, Maximiliano Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer |
title | Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer |
title_full | Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer |
title_fullStr | Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer |
title_full_unstemmed | Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer |
title_short | Metazoan Ribosome Inactivating Protein encoding genes acquired by Horizontal Gene Transfer |
title_sort | metazoan ribosome inactivating protein encoding genes acquired by horizontal gene transfer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5431988/ https://www.ncbi.nlm.nih.gov/pubmed/28500327 http://dx.doi.org/10.1038/s41598-017-01859-1 |
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