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Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus)
Skeletal muscle is capable of phenotypic adaptation to environmental factors, such as nutrient availability, by altering the balance between muscle catabolism and anabolism that in turn coordinates muscle growth. Small noncoding RNAs, known as microRNAs (miRNAs), repress the expression of target mRN...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5432107/ https://www.ncbi.nlm.nih.gov/pubmed/28505179 http://dx.doi.org/10.1371/journal.pone.0177679 |
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author | de Paula, Tassiana Gutierrez Zanella, Bruna Tereza Thomazini Fantinatti, Bruno Evaristo de Almeida de Moraes, Leonardo Nazário Duran, Bruno Oliveira da Silva de Oliveira, Caroline Bredariol Salomão, Rondinelle Artur Simões da Silva, Rafaela Nunes Padovani, Carlos Roberto dos Santos, Vander Bruno Mareco, Edson Assunção Carvalho, Robson Francisco Dal-Pai-Silva, Maeli |
author_facet | de Paula, Tassiana Gutierrez Zanella, Bruna Tereza Thomazini Fantinatti, Bruno Evaristo de Almeida de Moraes, Leonardo Nazário Duran, Bruno Oliveira da Silva de Oliveira, Caroline Bredariol Salomão, Rondinelle Artur Simões da Silva, Rafaela Nunes Padovani, Carlos Roberto dos Santos, Vander Bruno Mareco, Edson Assunção Carvalho, Robson Francisco Dal-Pai-Silva, Maeli |
author_sort | de Paula, Tassiana Gutierrez |
collection | PubMed |
description | Skeletal muscle is capable of phenotypic adaptation to environmental factors, such as nutrient availability, by altering the balance between muscle catabolism and anabolism that in turn coordinates muscle growth. Small noncoding RNAs, known as microRNAs (miRNAs), repress the expression of target mRNAs, and many studies have demonstrated that miRNAs regulate the mRNAs of catabolic and anabolic genes. We evaluated muscle morphology, gene expression of components involved in catabolism, anabolism and energetic metabolism and miRNAs expression in both the fast and slow muscle of juvenile pacu (Piaractus mesopotamicus) during food restriction and refeeding. Our analysis revealed that short periods of food restriction followed by refeeding predominantly affected fast muscle, with changes in muscle fiber diameter and miRNAs expression. There was an increase in the mRNA levels of catabolic pathways components (FBXO25, ATG12, BCL2) and energetic metabolism-related genes (PGC1α and SDHA), together with a decrease in PPARβ/δ mRNA levels. Interestingly, an increase in mRNA levels of anabolic genes (PI3K and mTORC1 complex: mTOR, mLST8 and RAPTOR) was also observed during food restriction. After refeeding, muscle morphology showed similar patterns of the control group; the majority of genes were slightly up- or down-regulated in fast and slow muscle, respectively; the levels of all miRNAs increased in fast muscle and some of them decreased in slow muscle. Our findings demonstrated that a short period of food restriction in juvenile pacu had a considerable impact on fast muscle, increasing the expression of anabolic (PI3K and mTORC1 complex: mTOR, mLST8 and RAPTOR) and energetic metabolism genes. The miRNAs (miR-1, miR-206, miR-199 and miR-23a) were more expressed during refeeding and while their target genes (IGF-1, mTOR, PGC1α and MAFbx), presented a decreased expression. The alterations in mTORC1 complex observed during fasting may have influenced the rates of protein synthesis by using amino acids from protein degradation as an alternative mechanism to preserve muscle phenotype and metabolic demand maintenance. |
format | Online Article Text |
id | pubmed-5432107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54321072017-05-26 Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) de Paula, Tassiana Gutierrez Zanella, Bruna Tereza Thomazini Fantinatti, Bruno Evaristo de Almeida de Moraes, Leonardo Nazário Duran, Bruno Oliveira da Silva de Oliveira, Caroline Bredariol Salomão, Rondinelle Artur Simões da Silva, Rafaela Nunes Padovani, Carlos Roberto dos Santos, Vander Bruno Mareco, Edson Assunção Carvalho, Robson Francisco Dal-Pai-Silva, Maeli PLoS One Research Article Skeletal muscle is capable of phenotypic adaptation to environmental factors, such as nutrient availability, by altering the balance between muscle catabolism and anabolism that in turn coordinates muscle growth. Small noncoding RNAs, known as microRNAs (miRNAs), repress the expression of target mRNAs, and many studies have demonstrated that miRNAs regulate the mRNAs of catabolic and anabolic genes. We evaluated muscle morphology, gene expression of components involved in catabolism, anabolism and energetic metabolism and miRNAs expression in both the fast and slow muscle of juvenile pacu (Piaractus mesopotamicus) during food restriction and refeeding. Our analysis revealed that short periods of food restriction followed by refeeding predominantly affected fast muscle, with changes in muscle fiber diameter and miRNAs expression. There was an increase in the mRNA levels of catabolic pathways components (FBXO25, ATG12, BCL2) and energetic metabolism-related genes (PGC1α and SDHA), together with a decrease in PPARβ/δ mRNA levels. Interestingly, an increase in mRNA levels of anabolic genes (PI3K and mTORC1 complex: mTOR, mLST8 and RAPTOR) was also observed during food restriction. After refeeding, muscle morphology showed similar patterns of the control group; the majority of genes were slightly up- or down-regulated in fast and slow muscle, respectively; the levels of all miRNAs increased in fast muscle and some of them decreased in slow muscle. Our findings demonstrated that a short period of food restriction in juvenile pacu had a considerable impact on fast muscle, increasing the expression of anabolic (PI3K and mTORC1 complex: mTOR, mLST8 and RAPTOR) and energetic metabolism genes. The miRNAs (miR-1, miR-206, miR-199 and miR-23a) were more expressed during refeeding and while their target genes (IGF-1, mTOR, PGC1α and MAFbx), presented a decreased expression. The alterations in mTORC1 complex observed during fasting may have influenced the rates of protein synthesis by using amino acids from protein degradation as an alternative mechanism to preserve muscle phenotype and metabolic demand maintenance. Public Library of Science 2017-05-15 /pmc/articles/PMC5432107/ /pubmed/28505179 http://dx.doi.org/10.1371/journal.pone.0177679 Text en © 2017 Paula et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article de Paula, Tassiana Gutierrez Zanella, Bruna Tereza Thomazini Fantinatti, Bruno Evaristo de Almeida de Moraes, Leonardo Nazário Duran, Bruno Oliveira da Silva de Oliveira, Caroline Bredariol Salomão, Rondinelle Artur Simões da Silva, Rafaela Nunes Padovani, Carlos Roberto dos Santos, Vander Bruno Mareco, Edson Assunção Carvalho, Robson Francisco Dal-Pai-Silva, Maeli Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) |
title | Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) |
title_full | Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) |
title_fullStr | Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) |
title_full_unstemmed | Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) |
title_short | Food restriction increase the expression of mTORC1 complex genes in the skeletal muscle of juvenile pacu (Piaractus mesopotamicus) |
title_sort | food restriction increase the expression of mtorc1 complex genes in the skeletal muscle of juvenile pacu (piaractus mesopotamicus) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5432107/ https://www.ncbi.nlm.nih.gov/pubmed/28505179 http://dx.doi.org/10.1371/journal.pone.0177679 |
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