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Agrobacterium tumefaciens-Mediated Transformation of Pseudocercospora fijiensis to Determine the Role of PfHog1 in Osmotic Stress Regulation and Virulence Modulation

Black Sigatoka disease, caused by Pseudocercospora fijiensis is a serious constraint to banana production worldwide. The disease continues to spread in new ecological niches and there is an urgent need to develop strategies for its control. The high osmolarity glycerol (HOG) pathway in Saccharomyces...

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Autores principales: Onyilo, Francis, Tusiime, Geoffrey, Chen, Li-Hung, Falk, Bryce, Stergiopoulos, Ioannis, Tripathi, Jaindra N., Tushemereirwe, Wilberforce, Kubiriba, Jerome, Changa, Charles, Tripathi, Leena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5432539/
https://www.ncbi.nlm.nih.gov/pubmed/28559879
http://dx.doi.org/10.3389/fmicb.2017.00830
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author Onyilo, Francis
Tusiime, Geoffrey
Chen, Li-Hung
Falk, Bryce
Stergiopoulos, Ioannis
Tripathi, Jaindra N.
Tushemereirwe, Wilberforce
Kubiriba, Jerome
Changa, Charles
Tripathi, Leena
author_facet Onyilo, Francis
Tusiime, Geoffrey
Chen, Li-Hung
Falk, Bryce
Stergiopoulos, Ioannis
Tripathi, Jaindra N.
Tushemereirwe, Wilberforce
Kubiriba, Jerome
Changa, Charles
Tripathi, Leena
author_sort Onyilo, Francis
collection PubMed
description Black Sigatoka disease, caused by Pseudocercospora fijiensis is a serious constraint to banana production worldwide. The disease continues to spread in new ecological niches and there is an urgent need to develop strategies for its control. The high osmolarity glycerol (HOG) pathway in Saccharomyces cerevisiae is well known to respond to changes in external osmolarity. HOG pathway activation leads to phosphorylation, activation and nuclear transduction of the HOG1 mitogen-activated protein kinases (MAPKs). The activated HOG1 triggers several responses to osmotic stress, including up or down regulation of different genes, regulation of protein translation, adjustments to cell cycle progression and synthesis of osmolyte glycerol. This study investigated the role of the MAPK-encoding PfHog1 gene on osmotic stress adaptation and virulence of P. fijiensis. RNA interference-mediated gene silencing of PfHog1 significantly suppressed growth of P. fijiensis on potato dextrose agar media supplemented with 1 M NaCl, indicating that PfHog1 regulates osmotic stress. In addition, virulence of the PfHog1-silenced mutants of P. fijiensis on banana was significantly reduced, as observed from the low rates of necrosis and disease development on the infected leaves. Staining with lacto phenol cotton blue further confirmed the impaired mycelial growth of the PfHog1 in the infected leaf tissues, which was further confirmed with quantification of the fungal biomass using absolute- quantitative PCR. Collectively, these findings demonstrate that PfHog1 plays a critical role in osmotic stress regulation and virulence of P. fijiensis on its host banana. Thus, PfHog1 could be an interesting target for the control of black Sigatoka disease in banana.
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spelling pubmed-54325392017-05-30 Agrobacterium tumefaciens-Mediated Transformation of Pseudocercospora fijiensis to Determine the Role of PfHog1 in Osmotic Stress Regulation and Virulence Modulation Onyilo, Francis Tusiime, Geoffrey Chen, Li-Hung Falk, Bryce Stergiopoulos, Ioannis Tripathi, Jaindra N. Tushemereirwe, Wilberforce Kubiriba, Jerome Changa, Charles Tripathi, Leena Front Microbiol Microbiology Black Sigatoka disease, caused by Pseudocercospora fijiensis is a serious constraint to banana production worldwide. The disease continues to spread in new ecological niches and there is an urgent need to develop strategies for its control. The high osmolarity glycerol (HOG) pathway in Saccharomyces cerevisiae is well known to respond to changes in external osmolarity. HOG pathway activation leads to phosphorylation, activation and nuclear transduction of the HOG1 mitogen-activated protein kinases (MAPKs). The activated HOG1 triggers several responses to osmotic stress, including up or down regulation of different genes, regulation of protein translation, adjustments to cell cycle progression and synthesis of osmolyte glycerol. This study investigated the role of the MAPK-encoding PfHog1 gene on osmotic stress adaptation and virulence of P. fijiensis. RNA interference-mediated gene silencing of PfHog1 significantly suppressed growth of P. fijiensis on potato dextrose agar media supplemented with 1 M NaCl, indicating that PfHog1 regulates osmotic stress. In addition, virulence of the PfHog1-silenced mutants of P. fijiensis on banana was significantly reduced, as observed from the low rates of necrosis and disease development on the infected leaves. Staining with lacto phenol cotton blue further confirmed the impaired mycelial growth of the PfHog1 in the infected leaf tissues, which was further confirmed with quantification of the fungal biomass using absolute- quantitative PCR. Collectively, these findings demonstrate that PfHog1 plays a critical role in osmotic stress regulation and virulence of P. fijiensis on its host banana. Thus, PfHog1 could be an interesting target for the control of black Sigatoka disease in banana. Frontiers Media S.A. 2017-05-16 /pmc/articles/PMC5432539/ /pubmed/28559879 http://dx.doi.org/10.3389/fmicb.2017.00830 Text en Copyright © 2017 Onyilo, Tusiime, Chen, Falk, Stergiopoulos, Tripathi, Tushemereirwe, Kubiriba, Changa and Tripathi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Onyilo, Francis
Tusiime, Geoffrey
Chen, Li-Hung
Falk, Bryce
Stergiopoulos, Ioannis
Tripathi, Jaindra N.
Tushemereirwe, Wilberforce
Kubiriba, Jerome
Changa, Charles
Tripathi, Leena
Agrobacterium tumefaciens-Mediated Transformation of Pseudocercospora fijiensis to Determine the Role of PfHog1 in Osmotic Stress Regulation and Virulence Modulation
title Agrobacterium tumefaciens-Mediated Transformation of Pseudocercospora fijiensis to Determine the Role of PfHog1 in Osmotic Stress Regulation and Virulence Modulation
title_full Agrobacterium tumefaciens-Mediated Transformation of Pseudocercospora fijiensis to Determine the Role of PfHog1 in Osmotic Stress Regulation and Virulence Modulation
title_fullStr Agrobacterium tumefaciens-Mediated Transformation of Pseudocercospora fijiensis to Determine the Role of PfHog1 in Osmotic Stress Regulation and Virulence Modulation
title_full_unstemmed Agrobacterium tumefaciens-Mediated Transformation of Pseudocercospora fijiensis to Determine the Role of PfHog1 in Osmotic Stress Regulation and Virulence Modulation
title_short Agrobacterium tumefaciens-Mediated Transformation of Pseudocercospora fijiensis to Determine the Role of PfHog1 in Osmotic Stress Regulation and Virulence Modulation
title_sort agrobacterium tumefaciens-mediated transformation of pseudocercospora fijiensis to determine the role of pfhog1 in osmotic stress regulation and virulence modulation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5432539/
https://www.ncbi.nlm.nih.gov/pubmed/28559879
http://dx.doi.org/10.3389/fmicb.2017.00830
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