Cargando…

Expression, purification and structural analysis of functional GABA transporter 1 using the baculovirus expression system

The γ-aminobutyric acid (GABA) transporter 1 (GAT1) belongs to a family of Na(+) and Cl(−)-coupled transport proteins and possesses 12 putative transmembrane domains. To perform structural analyses of the GAT1 protein, the GAT1/green fluorescent protein (GFP) fusion protein was functionally expresse...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hu, Jing, Weise, Chris, Böttcher, Christoph, Fan, Hua, Yin, Jian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Beilstein-Institut 2017
Materias:	Full Research Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433199/ https://www.ncbi.nlm.nih.gov/pubmed/28546845 http://dx.doi.org/10.3762/bjoc.13.88

Ejemplares similares

Expression and purification of a functional heteromeric GABA(A) receptor for structural studies
por: Claxton, Derek P., et al.
Publicado: (2018)

Expression and purification of human diacylglycerol kinase α from baculovirus-infected insect cells for structural studies
por: Takahashi, Daisuke, et al.
Publicado: (2018)

Expression and purification of MERS-CoV envelope protein, an essential viroporin, using the baculovirus expression system
por: Alsaadi, Entedar, et al.
Publicado: (2023)

Production and purification of VP2 protein of porcine parvovirus expressed in an insect-baculovirus cell system
por: Zhou, Hongchao, et al.
Publicado: (2010)

A baculovirus-mediated strategy for full-length plant virus coat protein expression and purification
por: Ardisson-Araújo, Daniel Mendes Pereira, et al.
Publicado: (2013)

Baculovirus and insect cell gene expression: review of baculovirus biotechnology.
por: Patterson, R M, et al.
Publicado: (1995)

Purification of baculovirus vectors using heparin affinity chromatography
por: Nasimuzzaman, Md, et al.
Publicado: (2016)

Expression, Delivery and Function of Insecticidal Proteins Expressed by Recombinant Baculoviruses
por: Kroemer, Jeremy A., et al.
Publicado: (2015)

Structure, function, and plasticity of GABA transporters
por: Scimemi, Annalisa
Publicado: (2014)

Establishment of a Novel Baculovirus–Silkworm Expression System
por: Wei, Junhong, et al.
Publicado: (2022)

A novel baculovirus-derived promoter with high activity in the baculovirus expression system
por: Martínez-Solís, María, et al.
Publicado: (2016)

Rapid, scalable, and low-cost purification of recombinant adeno-associated virus produced by baculovirus expression vector system
por: Buclez, Pierre-Olivier, et al.
Publicado: (2016)

Baculovirus expression: tackling the complexity challenge
por: Barford, David, et al.
Publicado: (2013)

Characteristic Expressions of GABA Receptors and GABA Producing/Transporting Molecules in Rat Kidney
por: Takano, Kozue, et al.
Publicado: (2014)

Structural, functional, and immunological characterization of bovine herpesvirus-1 glycoprotein gl expressed by recombinant baculovirus()
por: Van Drunen Littel-van den Hurk, S., et al.
Publicado: (1992)

Application of Baculovirus Expression Vector System (BEVS) in Vaccine Development
por: Hong, Qiaonan, et al.
Publicado: (2023)

Improving the baculovirus expression vector system with vankyrin‐enhanced technology
por: Steele, Kendra H., et al.
Publicado: (2017)

Study of Fusion Protein and Attachment Glycoprotein of Nipah Virus Expressed in Recombinant Baculovirus
por: WANG, Xi-Jun, et al.
Publicado: (2006)

The Baculoviruses Occlusion‐Derived Virus: Virion Structure and Function
por: Slack, Jeffery, et al.
Publicado: (2006)

Expressing of Recombinant VEGFR2-specific Nanobody in Baculovirus Expression System
por: Shokrollahi, Narjes, et al.
Publicado: (2021)

Baculovirus as a highly efficient expression vector in insect and mammalian cells
por: HU, Yu‐chen
Publicado: (2005)

Immunogenicity of the S protein of transmissible gastroenteritis virus expressed in baculovirus
por: Tuboly, T., et al.
Publicado: (1994)

New GABA amides activating GABA(A)-receptors
por: Raster, Peter, et al.
Publicado: (2013)

Baculovirus Expression and Functional Analysis of Vpa2 Proteins from Bacillus thuringiensis
por: Simón, Oihane, et al.
Publicado: (2020)

Acidic pH induces fusion of cells infected with baculovirus to form syncytia
por: Leikina, Evgenia, et al.
Publicado: (1992)

Production of CCHF virus-like particle by a baculovirus-insect cell expression system
por: Zhou, Zhao-rui, et al.
Publicado: (2011)

Budded baculovirus particle structure revisited
por: Wang, Qiushi, et al.
Publicado: (2016)

Baculovirus as versatile vectors for protein expression in insect and mammalian cells
por: Kost, Thomas A, et al.
Publicado: (2005)

Transcriptome analyses of insect cells to facilitate baculovirus-insect expression
por: Yu, Kai, et al.
Publicado: (2016)

Preventive, Diagnostic and Therapeutic Applications of Baculovirus Expression Vector System
por: Kumar, Neeraj, et al.
Publicado: (2017)

InteBac: An integrated bacterial and baculovirus expression vector suite
por: Altmannova, Veronika, et al.
Publicado: (2020)

Regulation of Glutamate, GABA and Dopamine Transporter Uptake, Surface Mobility and Expression
por: Ryan, Renae M., et al.
Publicado: (2021)

Expression of Functional Recombinant Human Tissue Transglutaminase (TG2) Using the Bac-to-Bac Baculovirus Expression System
por: Yazdani, Yaghoub, et al.
Publicado: (2016)

Binding of the baculovirus very late expression factor 1 (VLF-1) to different DNA structures
por: Mikhailov, Victor S, et al.
Publicado: (2002)

Expression and Function of GABA Receptors in Myelinating Cells
por: Serrano-Regal, Mari Paz, et al.
Publicado: (2020)

The Influence of SV40 polyA on Gene Expression of Baculovirus Expression Vector Systems
por: Salem, Tamer Z., et al.
Publicado: (2015)

Expression of Recombinant Phosphodiesterases 3A and 3B Using Baculovirus Expression System
por: Yan, Yongmin, et al.
Publicado: (2016)

Utility of Alternative Promoters for Foreign Gene Expression Using the Baculovirus Expression Vector System
por: Bruder, Mark R., et al.
Publicado: (2022)

Functional expression, purification and reconstitution of the recombinant phosphate transporter Pho89 of Saccharomyces cerevisiae
por: Sengottaiyan, Palanivelu, et al.
Publicado: (2013)

Purification of functional baculovirus particles from silkworm larval hemolymph and their use as nanoparticles for the detection of human prorenin receptor (PRR) binding
por: Kato, Tatsuya, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_4rj3ecrdqc7qpia1rmib2mb9qd