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Feeder & basic fibroblast growth factor-free culture of human embryonic stem cells: Role of conditioned medium from immortalized human feeders

BACKGROUND & OBJECTIVES: Human embryonic stem cell (hESC) lines are commonly maintained on inactivated feeder cells, in the medium supplemented with basic fibroblast growth factor (bFGF). However, limited availability of feeder cells in culture, and the high cost of growth factors limit their us...

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Detalles Bibliográficos
Autores principales:	Teotia, Pooja, Sharma, Shilpa, Airan, Balram, Mohanty, Sujata
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Medknow Publications & Media Pvt Ltd 2016
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433277/ https://www.ncbi.nlm.nih.gov/pubmed/28474621 http://dx.doi.org/10.4103/ijmr.IJMR_424_15

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