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Notch Signaling and Maintenance of SOX2 Expression in Rat Anterior Pituitary Cells

After publication of reports describing the presence of stem/progenitor cells among non-hormone-producing cells in the pituitary, the mechanism responsible for proliferation and differentiation generated considerable interest. Several studies have suggested that Notch signaling is involved. In the p...

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Autores principales: Batchuluun, Khongorzul, Azuma, Morio, Fujiwara, Ken, Yashiro, Takashi, Kikuchi, Motoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433936/
https://www.ncbi.nlm.nih.gov/pubmed/28522881
http://dx.doi.org/10.1267/ahc.17002
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author Batchuluun, Khongorzul
Azuma, Morio
Fujiwara, Ken
Yashiro, Takashi
Kikuchi, Motoshi
author_facet Batchuluun, Khongorzul
Azuma, Morio
Fujiwara, Ken
Yashiro, Takashi
Kikuchi, Motoshi
author_sort Batchuluun, Khongorzul
collection PubMed
description After publication of reports describing the presence of stem/progenitor cells among non-hormone-producing cells in the pituitary, the mechanism responsible for proliferation and differentiation generated considerable interest. Several studies have suggested that Notch signaling is involved. In the present study, we examined the histochemical relationship between Notch signaling molecules and the transcription factor SOX2 in rat pituitary. Combined in situ hybridization and immunohistochemistry showed that Notch2 mRNA and SOX2 were co-expressed at embryonic day 14.5 in most cells in the adenohypophyseal primordium. In adult rat pituitary, double immunohistochemistry showed that SOX2 and either Notch2 or the Notch signaling target HES1 were co-localized within cells with large oval nuclei in both the marginal cell layer and cell aggregates in the main part of the anterior lobe, which are believed to be stem cell niches. Furthermore, when the Notch signaling inhibitor DAPT was added to a primary culture of adult rat anterior pituitary cells, the proportion of SOX2-expressing cells within Notch2-positive cells was approximately 30% lower. These findings suggest that Notch signaling has a role in maintaining the stemness of precursor cells in the adult rat pituitary gland.
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spelling pubmed-54339362017-05-18 Notch Signaling and Maintenance of SOX2 Expression in Rat Anterior Pituitary Cells Batchuluun, Khongorzul Azuma, Morio Fujiwara, Ken Yashiro, Takashi Kikuchi, Motoshi Acta Histochem Cytochem Regular Article After publication of reports describing the presence of stem/progenitor cells among non-hormone-producing cells in the pituitary, the mechanism responsible for proliferation and differentiation generated considerable interest. Several studies have suggested that Notch signaling is involved. In the present study, we examined the histochemical relationship between Notch signaling molecules and the transcription factor SOX2 in rat pituitary. Combined in situ hybridization and immunohistochemistry showed that Notch2 mRNA and SOX2 were co-expressed at embryonic day 14.5 in most cells in the adenohypophyseal primordium. In adult rat pituitary, double immunohistochemistry showed that SOX2 and either Notch2 or the Notch signaling target HES1 were co-localized within cells with large oval nuclei in both the marginal cell layer and cell aggregates in the main part of the anterior lobe, which are believed to be stem cell niches. Furthermore, when the Notch signaling inhibitor DAPT was added to a primary culture of adult rat anterior pituitary cells, the proportion of SOX2-expressing cells within Notch2-positive cells was approximately 30% lower. These findings suggest that Notch signaling has a role in maintaining the stemness of precursor cells in the adult rat pituitary gland. JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2017-04-27 2017-04-15 /pmc/articles/PMC5433936/ /pubmed/28522881 http://dx.doi.org/10.1267/ahc.17002 Text en 2017 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Article
Batchuluun, Khongorzul
Azuma, Morio
Fujiwara, Ken
Yashiro, Takashi
Kikuchi, Motoshi
Notch Signaling and Maintenance of SOX2 Expression in Rat Anterior Pituitary Cells
title Notch Signaling and Maintenance of SOX2 Expression in Rat Anterior Pituitary Cells
title_full Notch Signaling and Maintenance of SOX2 Expression in Rat Anterior Pituitary Cells
title_fullStr Notch Signaling and Maintenance of SOX2 Expression in Rat Anterior Pituitary Cells
title_full_unstemmed Notch Signaling and Maintenance of SOX2 Expression in Rat Anterior Pituitary Cells
title_short Notch Signaling and Maintenance of SOX2 Expression in Rat Anterior Pituitary Cells
title_sort notch signaling and maintenance of sox2 expression in rat anterior pituitary cells
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433936/
https://www.ncbi.nlm.nih.gov/pubmed/28522881
http://dx.doi.org/10.1267/ahc.17002
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