Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins

BACKGROUND: Conditional knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional activators are key for many genetic studies and comprise over 90% of mouse models created. Conditional knockout mice are generated using labor-intensive methods of homologous r...

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Detalles Bibliográficos
Autores principales: Quadros, Rolen M., Miura, Hiromi, Harms, Donald W., Akatsuka, Hisako, Sato, Takehito, Aida, Tomomi, Redder, Ronald, Richardson, Guy P., Inagaki, Yutaka, Sakai, Daisuke, Buckley, Shannon M., Seshacharyulu, Parthasarathy, Batra, Surinder K., Behlke, Mark A., Zeiner, Sarah A., Jacobi, Ashley M., Izu, Yayoi, Thoreson, Wallace B., Urness, Lisa D., Mansour, Suzanne L., Ohtsuka, Masato, Gurumurthy, Channabasavaiah B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5434640/
https://www.ncbi.nlm.nih.gov/pubmed/28511701
http://dx.doi.org/10.1186/s13059-017-1220-4

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