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Characterization of the Fecal Bacterial Microbiota of Healthy and Diarrheic Dairy Calves

BACKGROUND: Neonatal diarrhea accounts for more than 50% of total deaths in dairy calves. Few population‐based studies of cattle have investigated how the microbiota is impacted during diarrhea. OBJECTIVES: To characterize the fecal microbiota and predict the functional potential of the microbial co...

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Autores principales: Gomez, D.E., Arroyo, L.G., Costa, M.C., Viel, L., Weese, J.S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435056/
https://www.ncbi.nlm.nih.gov/pubmed/28390070
http://dx.doi.org/10.1111/jvim.14695
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author Gomez, D.E.
Arroyo, L.G.
Costa, M.C.
Viel, L.
Weese, J.S.
author_facet Gomez, D.E.
Arroyo, L.G.
Costa, M.C.
Viel, L.
Weese, J.S.
author_sort Gomez, D.E.
collection PubMed
description BACKGROUND: Neonatal diarrhea accounts for more than 50% of total deaths in dairy calves. Few population‐based studies of cattle have investigated how the microbiota is impacted during diarrhea. OBJECTIVES: To characterize the fecal microbiota and predict the functional potential of the microbial communities in healthy and diarrheic calves. METHODS: Fifteen diarrheic calves between the ages of 1 and 30 days and 15 age‐matched healthy control calves were enrolled from 2 dairy farms. The Illumina MiSeq sequencer was used for high‐throughput sequencing of the V4 region of the 16S rRNA gene (Illumina, San Diego, CA). RESULTS: Significant differences in community membership and structure were identified among healthy calves from different farms. Differences in community membership and structure also were identified between healthy and diarrheic calves within each farm. Based on linear discriminant analysis effect size (LEfSe), the genera Bifidobacterium, Megamonas, and a genus of the family Bifidobacteriaceae were associated with health at farm 1, whereas Lachnospiraceae incertae sedis, Dietzia and an unclassified genus of the family Veillonellaceae were significantly associated with health at farm 2. The Phylogenetic Investigation of Communities Reconstruction of Unobserved States (PICRUSt) analysis indicated that diarrheic calves had decreased abundances of genes responsible for metabolism of various vitamins, amino acids, and carbohydrate. CLINICAL RELEVANCE: The fecal microbiota of healthy dairy calves appeared to be farm specific as were the changes observed during diarrhea. The differences in microbiota structure and membership between healthy and diarrheic calves suggest that dysbiosis can occur in diarrheic calves and it is associated with changes in predictive metagenomic function.
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spelling pubmed-54350562017-05-18 Characterization of the Fecal Bacterial Microbiota of Healthy and Diarrheic Dairy Calves Gomez, D.E. Arroyo, L.G. Costa, M.C. Viel, L. Weese, J.S. J Vet Intern Med FOOD AND FIBER ANIMAL BACKGROUND: Neonatal diarrhea accounts for more than 50% of total deaths in dairy calves. Few population‐based studies of cattle have investigated how the microbiota is impacted during diarrhea. OBJECTIVES: To characterize the fecal microbiota and predict the functional potential of the microbial communities in healthy and diarrheic calves. METHODS: Fifteen diarrheic calves between the ages of 1 and 30 days and 15 age‐matched healthy control calves were enrolled from 2 dairy farms. The Illumina MiSeq sequencer was used for high‐throughput sequencing of the V4 region of the 16S rRNA gene (Illumina, San Diego, CA). RESULTS: Significant differences in community membership and structure were identified among healthy calves from different farms. Differences in community membership and structure also were identified between healthy and diarrheic calves within each farm. Based on linear discriminant analysis effect size (LEfSe), the genera Bifidobacterium, Megamonas, and a genus of the family Bifidobacteriaceae were associated with health at farm 1, whereas Lachnospiraceae incertae sedis, Dietzia and an unclassified genus of the family Veillonellaceae were significantly associated with health at farm 2. The Phylogenetic Investigation of Communities Reconstruction of Unobserved States (PICRUSt) analysis indicated that diarrheic calves had decreased abundances of genes responsible for metabolism of various vitamins, amino acids, and carbohydrate. CLINICAL RELEVANCE: The fecal microbiota of healthy dairy calves appeared to be farm specific as were the changes observed during diarrhea. The differences in microbiota structure and membership between healthy and diarrheic calves suggest that dysbiosis can occur in diarrheic calves and it is associated with changes in predictive metagenomic function. John Wiley and Sons Inc. 2017-04-07 2017 /pmc/articles/PMC5435056/ /pubmed/28390070 http://dx.doi.org/10.1111/jvim.14695 Text en Copyright © 2017 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial (http://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle FOOD AND FIBER ANIMAL
Gomez, D.E.
Arroyo, L.G.
Costa, M.C.
Viel, L.
Weese, J.S.
Characterization of the Fecal Bacterial Microbiota of Healthy and Diarrheic Dairy Calves
title Characterization of the Fecal Bacterial Microbiota of Healthy and Diarrheic Dairy Calves
title_full Characterization of the Fecal Bacterial Microbiota of Healthy and Diarrheic Dairy Calves
title_fullStr Characterization of the Fecal Bacterial Microbiota of Healthy and Diarrheic Dairy Calves
title_full_unstemmed Characterization of the Fecal Bacterial Microbiota of Healthy and Diarrheic Dairy Calves
title_short Characterization of the Fecal Bacterial Microbiota of Healthy and Diarrheic Dairy Calves
title_sort characterization of the fecal bacterial microbiota of healthy and diarrheic dairy calves
topic FOOD AND FIBER ANIMAL
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435056/
https://www.ncbi.nlm.nih.gov/pubmed/28390070
http://dx.doi.org/10.1111/jvim.14695
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