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Comparative Sperm Proteomics in Mouse Species with Divergent Mating Systems
Sexual selection is the pervasive force underlying the dramatic divergence of sperm form and function. Although it has been demonstrated that testis gene expression evolves rapidly, exploration of the proteomic basis of sperm diversity is in its infancy. We have employed a whole-cell proteomics appr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435083/ https://www.ncbi.nlm.nih.gov/pubmed/28333336 http://dx.doi.org/10.1093/molbev/msx084 |
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author | Vicens, Alberto Borziak, Kirill Karr, Timothy L. Roldan, Eduardo R.S. Dorus, Steve |
author_facet | Vicens, Alberto Borziak, Kirill Karr, Timothy L. Roldan, Eduardo R.S. Dorus, Steve |
author_sort | Vicens, Alberto |
collection | PubMed |
description | Sexual selection is the pervasive force underlying the dramatic divergence of sperm form and function. Although it has been demonstrated that testis gene expression evolves rapidly, exploration of the proteomic basis of sperm diversity is in its infancy. We have employed a whole-cell proteomics approach to characterize sperm divergence among closely related Mus species that experience different sperm competition regimes and exhibit pronounced variation in sperm energetics, motility and fertilization capacity. Interspecific comparisons revealed significant abundance differences amongst proteins involved in fertilization capacity, including those that govern sperm-zona pellucida interactions, axoneme components and metabolic proteins. Ancestral reconstruction of relative testis size suggests that the reduction of zona pellucida binding proteins and heavy-chain dyneins was associated with a relaxation in sperm competition in the M. musculus lineage. Additionally, the decreased reliance on ATP derived from glycolysis in high sperm competition species was reflected in abundance decreases in glycolytic proteins of the principle piece in M. spretus and M. spicilegus. Comparison of protein abundance and stage-specific testis expression revealed a significant correlation during spermatid development when dynamic morphological changes occur. Proteins underlying sperm diversification were also more likely to be subject to translational repression, suggesting that sperm composition is influenced by the evolution of translation control mechanisms. The identification of functionally coherent classes of proteins relating to sperm competition highlights the utility of evolutionary proteomic analyses and reveals that both intensified and relaxed sperm competition can have a pronounced impact on the molecular composition of the male gamete. |
format | Online Article Text |
id | pubmed-5435083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-54350832017-05-22 Comparative Sperm Proteomics in Mouse Species with Divergent Mating Systems Vicens, Alberto Borziak, Kirill Karr, Timothy L. Roldan, Eduardo R.S. Dorus, Steve Mol Biol Evol Discoveries Sexual selection is the pervasive force underlying the dramatic divergence of sperm form and function. Although it has been demonstrated that testis gene expression evolves rapidly, exploration of the proteomic basis of sperm diversity is in its infancy. We have employed a whole-cell proteomics approach to characterize sperm divergence among closely related Mus species that experience different sperm competition regimes and exhibit pronounced variation in sperm energetics, motility and fertilization capacity. Interspecific comparisons revealed significant abundance differences amongst proteins involved in fertilization capacity, including those that govern sperm-zona pellucida interactions, axoneme components and metabolic proteins. Ancestral reconstruction of relative testis size suggests that the reduction of zona pellucida binding proteins and heavy-chain dyneins was associated with a relaxation in sperm competition in the M. musculus lineage. Additionally, the decreased reliance on ATP derived from glycolysis in high sperm competition species was reflected in abundance decreases in glycolytic proteins of the principle piece in M. spretus and M. spicilegus. Comparison of protein abundance and stage-specific testis expression revealed a significant correlation during spermatid development when dynamic morphological changes occur. Proteins underlying sperm diversification were also more likely to be subject to translational repression, suggesting that sperm composition is influenced by the evolution of translation control mechanisms. The identification of functionally coherent classes of proteins relating to sperm competition highlights the utility of evolutionary proteomic analyses and reveals that both intensified and relaxed sperm competition can have a pronounced impact on the molecular composition of the male gamete. Oxford University Press 2017-06 2017-02-25 /pmc/articles/PMC5435083/ /pubmed/28333336 http://dx.doi.org/10.1093/molbev/msx084 Text en © The Author 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Discoveries Vicens, Alberto Borziak, Kirill Karr, Timothy L. Roldan, Eduardo R.S. Dorus, Steve Comparative Sperm Proteomics in Mouse Species with Divergent Mating Systems |
title | Comparative Sperm Proteomics in Mouse Species with Divergent Mating Systems |
title_full | Comparative Sperm Proteomics in Mouse Species with Divergent Mating Systems |
title_fullStr | Comparative Sperm Proteomics in Mouse Species with Divergent Mating Systems |
title_full_unstemmed | Comparative Sperm Proteomics in Mouse Species with Divergent Mating Systems |
title_short | Comparative Sperm Proteomics in Mouse Species with Divergent Mating Systems |
title_sort | comparative sperm proteomics in mouse species with divergent mating systems |
topic | Discoveries |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435083/ https://www.ncbi.nlm.nih.gov/pubmed/28333336 http://dx.doi.org/10.1093/molbev/msx084 |
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