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Antimicrobial peptides extend lifespan in Drosophila
Antimicrobial peptides (AMPs) are important defense molecules of the innate immune system. High levels of AMPs are induced in response to infections to fight pathogens, whereas moderate levels induced by metabolic stress are thought to shape commensal microbial communities at barrier tissues. We exp...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435158/ https://www.ncbi.nlm.nih.gov/pubmed/28520752 http://dx.doi.org/10.1371/journal.pone.0176689 |
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author | Loch, Gerrit Zinke, Ingo Mori, Tetsushi Carrera, Pilar Schroer, Jonas Takeyama, Haruko Hoch, Michael |
author_facet | Loch, Gerrit Zinke, Ingo Mori, Tetsushi Carrera, Pilar Schroer, Jonas Takeyama, Haruko Hoch, Michael |
author_sort | Loch, Gerrit |
collection | PubMed |
description | Antimicrobial peptides (AMPs) are important defense molecules of the innate immune system. High levels of AMPs are induced in response to infections to fight pathogens, whereas moderate levels induced by metabolic stress are thought to shape commensal microbial communities at barrier tissues. We expressed single AMPs in adult flies either ubiquitously or in the gut by using the inducible GeneSwitch system to tightly regulate AMP expression. We found that activation of single AMPs, including Drosocin, resulted in a significant extension of Drosophila lifespan. These animals showed reduced activity of immune pathways over lifetime, less intestinal regenerative processes, reduced stress response and a delayed loss of gut barrier integrity. Furthermore, intestinal Drosocin induction protected the animals against infections with the natural Drosophila pathogen Pseudomonas entomophila, whereas a germ-reduced environment prevented the lifespan extending effect of Drosocin. Our study provides new insights into the crosstalk of innate immunity, intestinal homeostasis and ageing. |
format | Online Article Text |
id | pubmed-5435158 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54351582017-05-26 Antimicrobial peptides extend lifespan in Drosophila Loch, Gerrit Zinke, Ingo Mori, Tetsushi Carrera, Pilar Schroer, Jonas Takeyama, Haruko Hoch, Michael PLoS One Research Article Antimicrobial peptides (AMPs) are important defense molecules of the innate immune system. High levels of AMPs are induced in response to infections to fight pathogens, whereas moderate levels induced by metabolic stress are thought to shape commensal microbial communities at barrier tissues. We expressed single AMPs in adult flies either ubiquitously or in the gut by using the inducible GeneSwitch system to tightly regulate AMP expression. We found that activation of single AMPs, including Drosocin, resulted in a significant extension of Drosophila lifespan. These animals showed reduced activity of immune pathways over lifetime, less intestinal regenerative processes, reduced stress response and a delayed loss of gut barrier integrity. Furthermore, intestinal Drosocin induction protected the animals against infections with the natural Drosophila pathogen Pseudomonas entomophila, whereas a germ-reduced environment prevented the lifespan extending effect of Drosocin. Our study provides new insights into the crosstalk of innate immunity, intestinal homeostasis and ageing. Public Library of Science 2017-05-17 /pmc/articles/PMC5435158/ /pubmed/28520752 http://dx.doi.org/10.1371/journal.pone.0176689 Text en © 2017 Loch et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Loch, Gerrit Zinke, Ingo Mori, Tetsushi Carrera, Pilar Schroer, Jonas Takeyama, Haruko Hoch, Michael Antimicrobial peptides extend lifespan in Drosophila |
title | Antimicrobial peptides extend lifespan in Drosophila |
title_full | Antimicrobial peptides extend lifespan in Drosophila |
title_fullStr | Antimicrobial peptides extend lifespan in Drosophila |
title_full_unstemmed | Antimicrobial peptides extend lifespan in Drosophila |
title_short | Antimicrobial peptides extend lifespan in Drosophila |
title_sort | antimicrobial peptides extend lifespan in drosophila |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435158/ https://www.ncbi.nlm.nih.gov/pubmed/28520752 http://dx.doi.org/10.1371/journal.pone.0176689 |
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