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A multiplex TaqMan qPCR assay for sensitive and rapid detection of phytoplasmas infecting Rubus species
Rubus stunt is an economically important disease in the production of raspberries, blackberries, and loganberries. A fast, sensitive, and reliable diagnosis of phytoplasmas, the causal agent of the disease, is of prime importance to stop its spread by vegetative propagation and by insect vectors. Th...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435344/ https://www.ncbi.nlm.nih.gov/pubmed/28545043 http://dx.doi.org/10.1371/journal.pone.0177808 |
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author | Linck, Holger Krüger, Erika Reineke, Annette |
author_facet | Linck, Holger Krüger, Erika Reineke, Annette |
author_sort | Linck, Holger |
collection | PubMed |
description | Rubus stunt is an economically important disease in the production of raspberries, blackberries, and loganberries. A fast, sensitive, and reliable diagnosis of phytoplasmas, the causal agent of the disease, is of prime importance to stop its spread by vegetative propagation and by insect vectors. Therefore, multiplex qPCR assays using TaqMan probes with different kinds of fluorophores in one reaction were developed, allowing the detection of phytoplasmas in general as well as a more specific detection of phytoplasmas belonging to group 16SrV and host DNA (either plant or insect). This assay now provides a practical tool for the screening of motherplants and monitoring the presence and distribution of phytoplasmas in Rubus plants of different geographic origins, cultivars, and cultivation systems, as well as in putative insect vectors like leafhoppers. |
format | Online Article Text |
id | pubmed-5435344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54353442017-05-26 A multiplex TaqMan qPCR assay for sensitive and rapid detection of phytoplasmas infecting Rubus species Linck, Holger Krüger, Erika Reineke, Annette PLoS One Research Article Rubus stunt is an economically important disease in the production of raspberries, blackberries, and loganberries. A fast, sensitive, and reliable diagnosis of phytoplasmas, the causal agent of the disease, is of prime importance to stop its spread by vegetative propagation and by insect vectors. Therefore, multiplex qPCR assays using TaqMan probes with different kinds of fluorophores in one reaction were developed, allowing the detection of phytoplasmas in general as well as a more specific detection of phytoplasmas belonging to group 16SrV and host DNA (either plant or insect). This assay now provides a practical tool for the screening of motherplants and monitoring the presence and distribution of phytoplasmas in Rubus plants of different geographic origins, cultivars, and cultivation systems, as well as in putative insect vectors like leafhoppers. Public Library of Science 2017-05-17 /pmc/articles/PMC5435344/ /pubmed/28545043 http://dx.doi.org/10.1371/journal.pone.0177808 Text en © 2017 Linck et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Linck, Holger Krüger, Erika Reineke, Annette A multiplex TaqMan qPCR assay for sensitive and rapid detection of phytoplasmas infecting Rubus species |
title | A multiplex TaqMan qPCR assay for sensitive and rapid detection of phytoplasmas infecting Rubus species |
title_full | A multiplex TaqMan qPCR assay for sensitive and rapid detection of phytoplasmas infecting Rubus species |
title_fullStr | A multiplex TaqMan qPCR assay for sensitive and rapid detection of phytoplasmas infecting Rubus species |
title_full_unstemmed | A multiplex TaqMan qPCR assay for sensitive and rapid detection of phytoplasmas infecting Rubus species |
title_short | A multiplex TaqMan qPCR assay for sensitive and rapid detection of phytoplasmas infecting Rubus species |
title_sort | multiplex taqman qpcr assay for sensitive and rapid detection of phytoplasmas infecting rubus species |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435344/ https://www.ncbi.nlm.nih.gov/pubmed/28545043 http://dx.doi.org/10.1371/journal.pone.0177808 |
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