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Moderate Treadmill Exercise Training Improves Cardiovascular and Nitrergic Response and Resistance to Trypanosoma cruzi Infection in Mice

There is evidence suggesting that exercise training (ET) acts as a factor toward resistance to Trypanosoma cruzi infection. However, the effects of mean arterial pressure (MAP), heart rate (HR), and nitric oxide (NO) during the acute phase of infection has not been elucidated yet. Swiss mice were ra...

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Autores principales: Lucchetti, Bruno F. C., Zanluqui, Nágela G., de Ataides Raquel, Hiviny, Lovo-Martins, Maria I., Tatakihara, Vera L. H., de Oliveira Belém, Mônica, Michelini, Lisete C., de Almeida Araújo, Eduardo J., Pinge-Filho, Phileno, Martins-Pinge, Marli C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435761/
https://www.ncbi.nlm.nih.gov/pubmed/28572772
http://dx.doi.org/10.3389/fphys.2017.00315
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author Lucchetti, Bruno F. C.
Zanluqui, Nágela G.
de Ataides Raquel, Hiviny
Lovo-Martins, Maria I.
Tatakihara, Vera L. H.
de Oliveira Belém, Mônica
Michelini, Lisete C.
de Almeida Araújo, Eduardo J.
Pinge-Filho, Phileno
Martins-Pinge, Marli C.
author_facet Lucchetti, Bruno F. C.
Zanluqui, Nágela G.
de Ataides Raquel, Hiviny
Lovo-Martins, Maria I.
Tatakihara, Vera L. H.
de Oliveira Belém, Mônica
Michelini, Lisete C.
de Almeida Araújo, Eduardo J.
Pinge-Filho, Phileno
Martins-Pinge, Marli C.
author_sort Lucchetti, Bruno F. C.
collection PubMed
description There is evidence suggesting that exercise training (ET) acts as a factor toward resistance to Trypanosoma cruzi infection. However, the effects of mean arterial pressure (MAP), heart rate (HR), and nitric oxide (NO) during the acute phase of infection has not been elucidated yet. Swiss mice were randomly assigned into four groups: sedentary control (SC, n = 30), trained control (TC, n = 30), sedentary infected (SI, n = 30), and trained infected (TI, n = 30). ET was performed on the treadmill for 9 weeks. After training, the mice were infected with 5 × 10(3) trypomastigotes of T. cruzi (Y strain) or PBS. We observed resting bradycardia and improved performance in trained animals compared with sedentary ones. On the 20th day post-infection (DPI), we found a decrease in HR in SI animals compared to TI animals (699.73 ± 42.37 vs. 742.11 ± 25.35 bpm, respectively, P < 0.05). We also observed increased production of NO in cardiac tissue on the 20th DPI in the SI group, normalized in TI group (20.73 ± 2.74 vs. 6.51 ± 1.19 μM, respectively). Plasma pro-inflammatory cytokines (IL-12, TNF-α, IFN-γ,) and MCP-1 were increased in SI animals, but decreased in TI animals. The increase in parasitemia on the 15th and 17th DPI in the SI group was attenuated in the TI group. Our results suggest that previous ET plays a preventive role in resistance to T. cruzi infection, modulating cardiovascular aspects, inflammatory reaction, and NO levels of infected mice.
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spelling pubmed-54357612017-06-01 Moderate Treadmill Exercise Training Improves Cardiovascular and Nitrergic Response and Resistance to Trypanosoma cruzi Infection in Mice Lucchetti, Bruno F. C. Zanluqui, Nágela G. de Ataides Raquel, Hiviny Lovo-Martins, Maria I. Tatakihara, Vera L. H. de Oliveira Belém, Mônica Michelini, Lisete C. de Almeida Araújo, Eduardo J. Pinge-Filho, Phileno Martins-Pinge, Marli C. Front Physiol Physiology There is evidence suggesting that exercise training (ET) acts as a factor toward resistance to Trypanosoma cruzi infection. However, the effects of mean arterial pressure (MAP), heart rate (HR), and nitric oxide (NO) during the acute phase of infection has not been elucidated yet. Swiss mice were randomly assigned into four groups: sedentary control (SC, n = 30), trained control (TC, n = 30), sedentary infected (SI, n = 30), and trained infected (TI, n = 30). ET was performed on the treadmill for 9 weeks. After training, the mice were infected with 5 × 10(3) trypomastigotes of T. cruzi (Y strain) or PBS. We observed resting bradycardia and improved performance in trained animals compared with sedentary ones. On the 20th day post-infection (DPI), we found a decrease in HR in SI animals compared to TI animals (699.73 ± 42.37 vs. 742.11 ± 25.35 bpm, respectively, P < 0.05). We also observed increased production of NO in cardiac tissue on the 20th DPI in the SI group, normalized in TI group (20.73 ± 2.74 vs. 6.51 ± 1.19 μM, respectively). Plasma pro-inflammatory cytokines (IL-12, TNF-α, IFN-γ,) and MCP-1 were increased in SI animals, but decreased in TI animals. The increase in parasitemia on the 15th and 17th DPI in the SI group was attenuated in the TI group. Our results suggest that previous ET plays a preventive role in resistance to T. cruzi infection, modulating cardiovascular aspects, inflammatory reaction, and NO levels of infected mice. Frontiers Media S.A. 2017-05-18 /pmc/articles/PMC5435761/ /pubmed/28572772 http://dx.doi.org/10.3389/fphys.2017.00315 Text en Copyright © 2017 Lucchetti, Zanluqui, de Ataides Raquel, Lovo-Martins, Tatakihara, de Oliveira Belém, Michelini, de Almeida Araújo, Pinge-Filho and Martins-Pinge. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Lucchetti, Bruno F. C.
Zanluqui, Nágela G.
de Ataides Raquel, Hiviny
Lovo-Martins, Maria I.
Tatakihara, Vera L. H.
de Oliveira Belém, Mônica
Michelini, Lisete C.
de Almeida Araújo, Eduardo J.
Pinge-Filho, Phileno
Martins-Pinge, Marli C.
Moderate Treadmill Exercise Training Improves Cardiovascular and Nitrergic Response and Resistance to Trypanosoma cruzi Infection in Mice
title Moderate Treadmill Exercise Training Improves Cardiovascular and Nitrergic Response and Resistance to Trypanosoma cruzi Infection in Mice
title_full Moderate Treadmill Exercise Training Improves Cardiovascular and Nitrergic Response and Resistance to Trypanosoma cruzi Infection in Mice
title_fullStr Moderate Treadmill Exercise Training Improves Cardiovascular and Nitrergic Response and Resistance to Trypanosoma cruzi Infection in Mice
title_full_unstemmed Moderate Treadmill Exercise Training Improves Cardiovascular and Nitrergic Response and Resistance to Trypanosoma cruzi Infection in Mice
title_short Moderate Treadmill Exercise Training Improves Cardiovascular and Nitrergic Response and Resistance to Trypanosoma cruzi Infection in Mice
title_sort moderate treadmill exercise training improves cardiovascular and nitrergic response and resistance to trypanosoma cruzi infection in mice
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435761/
https://www.ncbi.nlm.nih.gov/pubmed/28572772
http://dx.doi.org/10.3389/fphys.2017.00315
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