Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids

Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM co...

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Autores principales: Engelen, Wouter, van de Wiel, Kayleigh M., Meijer, Lenny H. H., Saha, Bedabrata, Merkx, Maarten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436041/
https://www.ncbi.nlm.nih.gov/pubmed/28217801
http://dx.doi.org/10.1039/c6cc10032e
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author Engelen, Wouter
van de Wiel, Kayleigh M.
Meijer, Lenny H. H.
Saha, Bedabrata
Merkx, Maarten
author_facet Engelen, Wouter
van de Wiel, Kayleigh M.
Meijer, Lenny H. H.
Saha, Bedabrata
Merkx, Maarten
author_sort Engelen, Wouter
collection PubMed
description Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM concentrations of nucleic acids directly in complex media.
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spelling pubmed-54360412017-06-02 Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids Engelen, Wouter van de Wiel, Kayleigh M. Meijer, Lenny H. H. Saha, Bedabrata Merkx, Maarten Chem Commun (Camb) Chemistry Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM concentrations of nucleic acids directly in complex media. Royal Society of Chemistry 2017-03-07 2017-02-15 /pmc/articles/PMC5436041/ /pubmed/28217801 http://dx.doi.org/10.1039/c6cc10032e Text en This journal is © The Royal Society of Chemistry 2017 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Engelen, Wouter
van de Wiel, Kayleigh M.
Meijer, Lenny H. H.
Saha, Bedabrata
Merkx, Maarten
Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids
title Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids
title_full Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids
title_fullStr Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids
title_full_unstemmed Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids
title_short Nucleic acid detection using BRET-beacons based on bioluminescent protein–DNA hybrids
title_sort nucleic acid detection using bret-beacons based on bioluminescent protein–dna hybrids
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436041/
https://www.ncbi.nlm.nih.gov/pubmed/28217801
http://dx.doi.org/10.1039/c6cc10032e
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